Molecular mechanisms of signal transduction in macrophages

Hamilton, Thomas A.; Adams, Dolph O.

doi:10.1016/0167-5699(87)90145-9

Cited by 332 publications

(113 citation statements)

References 67 publications

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“…Inflammatory activation of macrophages by IFN-␥, TNF-␣, and LPS comprises two stages, an initial response to priming agents like PMA or IFN-␥, and a second phase in response to triggering agents like TNF-␣ or LPS, which elicit the full inflammatory phenotype [36]. Fully activated cells have upregulated phagocytic capacity and exhibit increased secretion of bactericidal mediators, cytokines and prostaglandins.…”

Section: Discussionmentioning

confidence: 99%

Modulation of P2X7 nucleotide receptor expression by pro- and anti-inflammatory stimuli in THP-1 monocytes

Humphreys

Dubyak

1998

Journal of Leukocyte Biology

138

115

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Section: Discussionmentioning

confidence: 99%

Modulation of P2X7 nucleotide receptor expression by pro- and anti-inflammatory stimuli in THP-1 monocytes

Humphreys

Dubyak

1998

Journal of Leukocyte Biology

138

115

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“…[1]. When monocytes are exposed in vitro to immunologic or inflammatory stimuli such as interferon gamma, LPS or TNF, an induction of homotypic adhesions occurs concomitantly with activation and differentiation of these cells [2][3][4]. Similarly, phor bol esters induce cellular differentiation as well as an increase in intercellular adhesions, as evidenced by cell cluster formation, in the human promonocytic U-937 cells and in the myelomonocytic cell line HL-60 [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Induction of LFA-1-mediated homotypic adhesions in promonocytic U-937 cells occurs independently of cell differentiation

Cabañas

Lastres

Bellón

et al. 1991

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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“…LPS plus A23187, PMA plus A23187. and LPS plus y-IFN are examples of stimuli which activate protein kinase C as well as inducing tumouricidal function in macrophages (2,7,8,11). In a previous study, Didier el al.…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation of cytoskeleton‐associated proteins, pp58 and pp60, in tumouricidal murine peritoneal macrophages

Didier

Fan²,

Wheeler³

et al. 1989

Immunol Cell Biol

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Summary Two highly phosphorylated vimentin-like proteins. pp58 and pp60. are expressed in macrophages activated in vivo to tumouricidal activity. Resident and elicited, non-tumouricidal peritoneal macrophages displayed low and intermediate levels of phosphorylated pp58 and pp60. respectively. C3H/HeN macrophages became tumouricidal after incubation with 01 |ig/mL A23I87 plus 10 nmol/L 12-phorbol 13-myristate acetate (PMA), or O'l (ig/niL A23187 plus 100 ng/mL lipopolysaccharide (LPS), and displayed increased phosphorylation of pp58 and pp60. LPS non-responder C3H/HeJ macrophages were nol tumouricidal nor did they show increased phosphorylation of pp58 and pp60 after incubation with LPS plus A23187 in vitro. C3H/HeJ macrophages. however, did become tumouricidal and expressed increased phosphorylation orpp58 and pp60 after incubation with A23187 and PMA. Addition of PGE2 (10"** mol/L), resulted in down-regulation of macrophage tumouricidal activity and decreased pp58 and pp60 phosphorylation. which was reversed by addition of indomethacin (10"'' mol/L) to cultures with PGE2. Phosphorylation increased within 5 min after adding activating stimuli while incorporation of [-'^S]-methionine into a 58 kD protein did not occur until 6 h later. No 60 kD protein synthesis was detected during the first 8 h after adding activating stimuli, indicating that previously synthesized proteins were phosphorylated during macrophage activation. These results signify a physiological role for the phosphorylation of cytoskeleton-associated pp58 and pp60 during macrophage activation to tumour cytotoxicity.

show abstract

Molecular mechanisms of signal transduction in macrophages

Cited by 332 publications

References 67 publications

Modulation of P2X7 nucleotide receptor expression by pro- and anti-inflammatory stimuli in THP-1 monocytes

Modulation of P2X7 nucleotide receptor expression by pro- and anti-inflammatory stimuli in THP-1 monocytes

Induction of LFA-1-mediated homotypic adhesions in promonocytic U-937 cells occurs independently of cell differentiation

Phosphorylation of cytoskeleton‐associated proteins, pp58 and pp60, in tumouricidal murine peritoneal macrophages

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