Molecular Mechanisms of Sex Pheromone Reception in Moths

Shiota, Yusuke; Sakurai, Takeshi

doi:10.1007/978-981-15-3082-1_9

Cited by 3 publications

(1 citation statement)

References 97 publications

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“…Hemipterans and holometabolous moths are sister lineages (Kristensen, 1991). Species in these two lineages usually rely on female-produced sex pheromones for mating (Millar, 2005;Shiota & Sakurai, 2020). Therefore, it is of interest to investigate PBP evolution in lepidopteran moths and hemipteran mirid bugs.…”

Section: Discussionmentioning

confidence: 99%

The Adelphocoris lineolatus OBP4: Support for evolutionary and functional divergence of a mirid pheromone‐binding protein from that found in lepidopteran moths

Wang

et al. 2021

Insect Science

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Pheromone-binding proteins (PBPs) have been extensively investigated in lepidopteran moths, but their evolution and function in hemipteran species remain unclear. Our previous study demonstrated that an odorant-binding protein, OBP4, of the mirid bug Adelphocoris lineolatus functions as a candidate hemipteran PBP but clustered with lepidopteran antennae-binding proteins (ABPs) rather than in the PBP/general odorantbinding protein (GOBP) clade. In this study, we hypothesized that origin and function of PBPs in hemipteran bugs may differ from those of lepidopteran moths. To test this hypothesis, we first constructed a phylogenetic tree using insect OBPs from sister hemipteran and holometabolous lineages, and the results indicated that neither OBP4 nor other types of candidate PBPs of mirid bugs clustered with the lepidopteran PBP/GOBP clade. Then, a fluorescence competitive binding assay was employed to determine binding affinities of recombinant OBP4 protein to host plant volatiles, with functional groups different from A. lineolatus sex pheromone components. The results revealed that OBP4 highly bound the female adult attractant 3-hexanone and 15 other mirid bug biologically active plant volatiles. Finally, we examined cellular expression profiles of OBP4 in putative antennal sensilla that are related to female A. lineolatus host plant location. The fluorescence in situ hybridization and immunocytochemical labeling assay showed that the OBP4 gene was highly expressed in the multiporous olfactory sensilla medium-long sensilla basiconica rather than in the short sensilla basiconica or uniporous sensilla chaetica. These results, together with those of our previous studies, indicate that OBP4 not only functions in recognition of bug-produced sex pheromones in males, but is probably involved in detection of host plant volatiles in both A. lineolatus sexes. Our findings support the hypothesis that the origin and function of PBPs in hemipteran bugs differ from those of well-known PBPs in lepidopteran moths, which provides a novel perspective on evolutionary mechanisms of sex pheromone communication across insect orders.

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Section: Discussionmentioning

confidence: 99%

The Adelphocoris lineolatus OBP4: Support for evolutionary and functional divergence of a mirid pheromone‐binding protein from that found in lepidopteran moths

Wang

et al. 2021

Insect Science

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Verbenone—the universal bark beetle repellent? Its origin, effects, and ecological roles

et al. 2023

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Bark beetles (Curculionidae: Scolytinae) spend most of their life in tissues of host plants, with several species representing economically relevant pests. Their behaviour is largely guided by complex olfactory cues. The compound verbenone was discovered early in the history of bark beetle pheromone research and is now sometimes referred to as a ‘universal bark beetle repellent’. However, some studies aiming to protect trees with verbenone have failed. In fact, most research effort has gone into applied studies, leaving many questions regarding the ecological functions of verbenone for various species unanswered. Here, we review and analyse the scientific literature from more than 50 years. Behavioural responses to verbenone are common among pest bark beetles (< 1% of scolytine species studied so far). Indeed, attraction is inhibited in 38 species from 16 genera, while some secondary species are unaffected or even attracted to verbenone. It is not clear whether the beetles can control the biosynthesis of verbenone; its release may not be an active signal by the beetles, but a passive cue resulting from microorganisms during host colonisation. In this context, we advocate to recognise a bark beetle and its microbiome as an entity (‘holobiont’), to better understand temporal release patterns and deduce the specific function of verbenone for a given species. Surprisingly, natural enemies are not commonly attracted by verbenone, but more taxa need to be studied. A better understanding of the ecological functions of verbenone will help to make verbenone-based tools more effective and improve integrated pest management strategies.

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Structural evidence for pheromone discrimination by the pheromone binding protein 3 from Plutella xylostella

Liu

Zhou

et al. 2021

International Journal of Biological Macromolecules

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Molecular Mechanisms of Sex Pheromone Reception in Moths

Cited by 3 publications

References 97 publications

The Adelphocoris lineolatus OBP4: Support for evolutionary and functional divergence of a mirid pheromone‐binding protein from that found in lepidopteran moths

The Adelphocoris lineolatus OBP4: Support for evolutionary and functional divergence of a mirid pheromone‐binding protein from that found in lepidopteran moths

Verbenone—the universal bark beetle repellent? Its origin, effects, and ecological roles

Structural evidence for pheromone discrimination by the pheromone binding protein 3 from Plutella xylostella

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