Molecular mechanisms of phosphate transport in plants

Rausch, Christine; Bucher, Marcel

doi:10.1007/s00425-002-0921-3

Cited by 451 publications

(352 citation statements)

References 124 publications

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“…In addition, increased root surface area is achieved by a combination of reduced root diameter and enhanced elongation of relatively thinner roots (Fitter et al, 2002). There is both intraspecific and interspecific genetic variation for P deficiency tolerance in crop species (Lynch andBrown, 2001, 2012;Mudge et al, 2002;Paszkowski et al, 2002;Rausch and Bucher, 2002;Huang et al, 2011;Zhang et al, 2011;Leiser et al, 2014a) that can be explored to develop P-efficient cultivars.…”

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Duplicate and Conquer: Multiple Homologs ofPHOSPHORUS-STARVATION TOLERANCE1Enhance Phosphorus Acquisition and Sorghum Performance on Low-Phosphorus Soils

et al. 2014

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Low soil phosphorus (P) availability is a major constraint for crop production in tropical regions. The rice (Oryza sativa) protein kinase, PHOSPHORUS-STARVATION TOLERANCE1 (OsPSTOL1), was previously shown to enhance P acquisition and grain yield in rice under P deficiency. We investigated the role of homologs of OsPSTOL1 in sorghum (Sorghum bicolor) performance under low P. Association mapping was undertaken in two sorghum association panels phenotyped for P uptake, root system morphology and architecture in hydroponics and grain yield and biomass accumulation under low-P conditions, in Brazil and/or in Mali. Root length and root surface area were positively correlated with grain yield under low P in the soil, emphasizing the importance of P acquisition efficiency in sorghum adaptation to low-P availability. SbPSTOL1 alleles reducing root diameter were associated with enhanced P uptake under low P in hydroponics, whereas Sb03g006765 and Sb03g0031680 alleles increasing root surface area also increased grain yield in a low-P soil. SbPSTOL1 genes colocalized with quantitative trait loci for traits underlying root morphology and dry weight accumulation under low P via linkage mapping. Consistent allelic effects for enhanced sorghum performance under low P between association panels, including enhanced grain yield under low P in the soil in Brazil, point toward a relatively stable role for Sb03g006765 across genetic backgrounds and environmental conditions. This study indicates that multiple SbPSTOL1 genes have a more general role in the root system, not only enhancing root morphology traits but also changing root system architecture, which leads to grain yield gain under low-P availability in the soil.

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Duplicate and Conquer: Multiple Homologs ofPHOSPHORUS-STARVATION TOLERANCE1Enhance Phosphorus Acquisition and Sorghum Performance on Low-Phosphorus Soils

et al. 2014

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“…However, most soil Pi is immobile and the Pi concentration available to roots is in micromolar quantities (Raghothama, 1999). To coordinate plant growth with the limited Pi availability, highaffinity Pi transporters have evolved to enable increased Pi acquisition from soils (Raghothama, 1999;Paszkowski et al, 2002;Rausch and Bucher, 2002;Ticconi and Abel, 2004). High-affinity plant Pi transporters were originally identified by sequence similarity with the high-affinity transporter of yeast (Saccharomyces cerevisiae), PHO84.…”

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“…High-affinity plant Pi transporters were originally identified by sequence similarity with the high-affinity transporter of yeast (Saccharomyces cerevisiae), PHO84. Genes encoding some of these transporters are able to complement pho84 yeast mutants (Rausch and Bucher, 2002). These proteins belong to the PHOSPHATE TRANSPORTER1 (PHT1) family of Pi/H + symporters (Rausch and Bucher, 2002).…”

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OsPHF1 Regulates the Plasma Membrane Localization of Low- and High-Affinity Inorganic Phosphate Transporters and Determines Inorganic Phosphate Uptake and Translocation in Rice

et al. 2011

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PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (PHF1) is known to regulate the plasma membrane localization of PHT1;1, a high-affinity inorganic phosphate (Pi) transporter in Arabidopsis (Arabidopsis thaliana). OsPHF1, a rice (Oryza sativa) gene homologous to AtPHF1, was isolated and found to regulate the localization of both low-and high-affinity Pi transporters to the plasma membrane. Three OsPHF1 allelic mutants carrying one-point mutations at the fifth WD-repeat motif and two at the transmembrane helix, respectively, showed arsenate resistance and severely reduced Pi accumulation. The data indicate that mutation of OsPHF1 results in the endoplasmic reticulum retention of the low-affinity Pi transporter OsPT2 and highaffinity Pi transporter OsPT8. Mutation of OsPHF1 also reduced Pi accumulation in plants exhibiting excessive shoot Pi accumulation due to the overexpression of OsPHR2. However, the transcript level of OsPHF1 itself is not controlled by OsPHR2. Overexpression of OsPHF1 increased Pi accumulation in both roots and shoots in a solution culture with Pi-supplied condition. These results indicate that the role of OsPHF1 is unique in the localization of both low-and high-affinity Pi transporters on the plasma membrane in rice and determines Pi uptake and translocation in rice. The similar function of PHF1 required to facilitate PHT1 transit through the endoplasmic reticulum between Arabidopsis and rice provides an example of expectations from what one would deduce from sequence comparisons to extend knowledge from Arabidopsis to crops.

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“…Pi transporters belonging to two major gene families (Pht1 and Pht2) have now been identified for a range of plant species (for review, see Rausch and Bucher, 2002) and have been most extensively studied in Arabidopsis (Arabidopsis thaliana; Muchhal et al, 1996;Mitsukawa et al, 1997;Smith et al, 1997Smith et al, , 2000Okumura et al, 1998;Karthikeyan et al, 2002;Mudge et al, 2002). The members of the gene family that are expressed in roots are typically up-regulated under P deficiency, but the molecular basis of the regulation has not been investigated.…”

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Promoter Analysis of the Barley Pht1;1 Phosphate Transporter Gene Identifies Regions Controlling Root Expression and Responsiveness to Phosphate Deprivation

et al. 2004

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Previous studies have shown that the promoter from the barley (Hordeum vulgare) phosphate transporter gene, HvPht1;1, activates high levels of expression in rice (Oryza sativa) roots and that the expression level was induced by up to 4-fold in response to phosphorus (P) deprivation. To identify promoter regions controlling gene regulation specificities, successive promoter truncations were made and attached to reporter genes. Promoters of between 856 and 1,400 nucleotides activated gene expression in a number of cell types but with maximal expression in trichoblast (root hair) cells. For shorter promoters the trichoblast specificity was lost, but in other tissues the distribution pattern was unchanged. The low P induction response was unaffected by promoter length. Domain exchange experiments subsequently identified that the region between 2856 and 2547 nucleotides (relative to the translational start) is required for epidermal cell expression. A second region located between 0 and 2195 nucleotides controls root-tip expression. The HvPht1;1 promoter contains one PHO-like motif and three motifs similar to the dicot P1BS element. Analysis of promoters from which the PHO-like element was eliminated (by truncation) showed no change in the gene induction response to P deficiency. In contrast, mutation of the P1BS elements eliminated any induction of gene expression in response to low P. An internal HvPht1;1 promoter fragment, incorporating a single P1BS element, had an increased response to P deprivation in comparison with the unmodified promoter (containing three elements). Together these findings further our understanding of the regulation of the HvPht1;1 gene and provide direct evidence for a functional role of the P1BS element in the expression of P-regulated genes.

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Molecular mechanisms of phosphate transport in plants

Cited by 451 publications

References 124 publications

Duplicate and Conquer: Multiple Homologs ofPHOSPHORUS-STARVATION TOLERANCE1Enhance Phosphorus Acquisition and Sorghum Performance on Low-Phosphorus Soils

Duplicate and Conquer: Multiple Homologs ofPHOSPHORUS-STARVATION TOLERANCE1Enhance Phosphorus Acquisition and Sorghum Performance on Low-Phosphorus Soils

OsPHF1 Regulates the Plasma Membrane Localization of Low- and High-Affinity Inorganic Phosphate Transporters and Determines Inorganic Phosphate Uptake and Translocation in Rice

Promoter Analysis of the Barley Pht1;1 Phosphate Transporter Gene Identifies Regions Controlling Root Expression and Responsiveness to Phosphate Deprivation

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