Molecular mechanisms of <i>Piriformospora indica</i> mediated growth promotion in plants

Kundu, Anish; Vadassery, Jyothilakshmi

doi:10.1080/15592324.2022.2096785

Cited by 14 publications

(11 citation statements)

References 33 publications

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“…In the current studies axenically cultured P. indica was inoculated to black pepper roots to examine its effects on growth and the expression of genes controlling flowering time. P. indica has been reported to interfere with the biosynthesis of phytohormones and signalling to trigger growth & differentiation ( Liu et al., 2019 ; Kundu and Vadassery, 2022 ), floral induction ( Pan et al., 2017 ) and immune responses ( Li et al., 2022 ). During colonization by the endophytes, plants try to regulate the hormone levels in the roots inorder to control their colonization and propagation.…”

Section: Discussionmentioning

confidence: 99%

Root colonization by the endophytic fungus Piriformospora indica shortens the juvenile phase of Piper nigrum L. by fine tuning the floral promotion pathways

Lekshmi

Sora²,

Anith³

et al. 2022

Front. Plant Sci.

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Piriformospora indica, the mutualistic biotrophic root colonizing endosymbiotic fungus belonging to the order Sebacinales, offers host plants various benefits and enhances its growth and performance. The effect of colonization of P. indica in Piper nigrum L. cv. Panniyur1 on growth advantages, floral induction and evocation was investigated. Growth and yield benefits are credited to the alteration in the phytohormone levels fine-tuned by plants in response to the fungal colonization and perpetuation. The remarkable upregulation in the phytohormone levels, as estimated by LC- MS/MS and quantified by qRT-PCR, revealed the effectual contribution by the endophyte. qRT-PCR results revealed a significant shift in the expression of putative flowering regulatory genes in the photoperiod induction pathway (FLOWERING LOCUS T, LEAFY, APETALA1, AGAMOUS, SUPPRESSOR OF CONSTANS 1, GIGANTEA, PHYTOCHROMEA, and CRYPTOCHROME1) gibberellin biosynthetic pathway genes (GIBBERELLIN 20-OXIDASE2, GIBBERELLIN 2-OXIDASE, DELLA PROTEIN REPRESSOR OF GA1-3 1) autonomous (FLOWERING LOCUS C, FLOWERING LOCUS VE, FLOWERING LOCUS CA), and age pathway (SQUAMOSA PROMOTER LIKE9, APETALA2). The endophytic colonization had no effect on vernalization (FLOWERING LOCUS C) or biotic stress pathways (SALICYLIC ACID INDUCTION DEFICIENT 2, WRKY family transcription factor 22). The data suggest that P. nigrum responds positively to P. indica colonization, affecting preponement in floral induction as well as evocation, and thereby shortening the juvenile phase of the crop.

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Section: Discussionmentioning

confidence: 99%

Root colonization by the endophytic fungus Piriformospora indica shortens the juvenile phase of Piper nigrum L. by fine tuning the floral promotion pathways

Lekshmi

Sora²,

Anith³

et al. 2022

Front. Plant Sci.

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“…P. indica is well known to be able to establish beneficial interactions with many different hosts, including monocotyledons such as barley, wheat, rice, corn, and dicotyledons such as Arabidopsis and tobacco [ 8 ], even on Brassicaceae family that cannot be colonized by mycorrhizal fungi [ 11 ]. Colonization of the roots by P. indica results in enhanced biomass production as well as increased resistance against biotic and abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

“…Piriformospora indica ( P. indica ) is an endophytic fungus, belonging to the order Sebacinales that colonizes the roots of both monocotyledons and dicotyledons plants [ 5 ]. P. indica serves as an excellent model for beneficial microbes as it can form a mutually beneficial symbiosis with a series of crops such as Chinese cabbage, rice, wheat, cucumber, onion and banana, which can effectively promote their growth, nutrient absorption, accumulation of secondary metabolites, and resistance to disease damage [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. P. indica has great potential in biological control and soil improvement and thus can play a positive role in agricultural production.…”

Section: Introductionmentioning

confidence: 99%

Reprogramming of Fundamental miRNA and Gene Expression during the Barley-Piriformospora indica Interaction

Guo

Zhang

et al. 2022

JoF

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The interactions between plants and microorganisms, which are widely present in the microbial-dominated rhizosphere, have been studied. This association is highly beneficial to the organisms involved, as plants benefit soil microorganisms by providing them with metabolites, while microorganisms promote plant growth and development by promoting nutrient uptake and/or protecting the plant from biotic and abiotic stresses. Piriformospora indica, an endophytic fungus of Sebacinales, colonizes the roots of a wide range of host plants and establishes various benefits for the plants. In this work, an interaction between barley and the P. indica was established to elucidate microRNA (miRNA)-based regulatory changes in miRNA profiles and gene expression that occurred during the symbiosis. Growth promotion and vigorous root development were confirmed in barley colonized by P. indica. The genome-wide expression profile analysis of miRNAs in barley root showed that 7,798,928, 6,418,039 and 7,136,192 clean reads were obtained from the libraries of mock, 3 dai and 7 dai roots, respectively. Sequencing of the barley genome yielded in 81 novel miRNA and 450 differently expressed genes (DEGs). Additionally, 11, 24, 6 differentially expressed microRNAs (DEMs) in barley were found in the three comparison groups, including 3 dai vs. mock, 7 dai vs. mock and 7 dai vs. 3 dai, respectively. The predicted target genes of these miRNAs are mainly involved in transcription, cell division, auxin signal perception and transduction, photosynthesis and hormone stimulus. Transcriptome analysis of P. indica identified 667 and 594 differentially expressed genes (DEG) at 3 dai and 7 dai. Annotation and GO (Gene Ontology) analysis indicated that the DEGs with the greatest changes were concentrated in oxidoreductase activity, ion transmembrane transporter activity. It implies that reprogramming of fundamental miRNA and gene expression occurs both in barley and P. indica. Analysis of global changes in miRNA profiles of barley colonized with P. indica revealed that several putative endogenous barley miRNAs expressed upon colonization belonging to known micro RNA families involved in growth and developmental regulation.

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“…[94,104] AUX signalling modulation and production by Si is important for altering root development and involved in growth promoting effects. [105] Besides changing hormone signalling, Si increases hormone levels/hormone production in hosts like AUX, ABA, SA and GA in addition to betaine, glycine, and proline. Increased levels of theses hormones and molecules were shown to improve plant resistance to environmental stresses, like salinity.…”

Section: Symbiont Effectors Identify Beneficial Genetic Traits In Hig...mentioning

confidence: 99%

Symbiont effector‐guided mapping of proteins in plant networks to improve crop climate stress resilience

Rehneke,

Schäfer

2024

BioEssays

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There is an urgent need for novel protection strategies to sustainably secure crop production under changing climates. Studying microbial effectors, defined as microbe‐derived proteins that alter signalling inside plant cells, has advanced our understanding of plant immunity and microbial plant colonisation strategies. Our understanding of effectors in the establishment and beneficial outcome of plant symbioses is less well known. Combining functional and comparative interaction assays uncovered specific symbiont effector targets in highly interconnected plant signalling networks and revealed the potential of effectors in beneficially modulating plant traits. The diverse functionality of symbiont effectors differs from the paradigmatic immuno‐suppressive function of pathogen effectors. These effectors provide solutions for improving crop resilience against climate stress by their evolution‐driven specification in host protein targeting and modulation. Symbiont effectors represent stringent tools not only to identify genetic targets for crop breeding, but to serve as applicable agents in crop management strategies under changing environments.

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Molecular mechanisms of Piriformospora indica mediated growth promotion in plants

Cited by 14 publications

References 33 publications

Root colonization by the endophytic fungus Piriformospora indica shortens the juvenile phase of Piper nigrum L. by fine tuning the floral promotion pathways

Root colonization by the endophytic fungus Piriformospora indica shortens the juvenile phase of Piper nigrum L. by fine tuning the floral promotion pathways

Reprogramming of Fundamental miRNA and Gene Expression during the Barley-Piriformospora indica Interaction

Symbiont effector‐guided mapping of proteins in plant networks to improve crop climate stress resilience

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