Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11

Matsumoto, M.; Sakae, Kenji; Ohta, Michio; Endo, Miyoko; Okuno, Rumi; Murayama, Shoko; Hirasawa, Kyoko; Suzuki, Riéko; Isobe, Junko; Tanaka, Daisuke; Katsukawa, Chihiro; Tamaru, Aki; Tomita, Masaaki; Ogata, Kikuyo; Yasuoka, Tomihisa; Ikebe, Tadayoshi; Watanabe, Haruo

doi:10.1016/j.ijantimicag.2004.09.014

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…A combination of a ␤-lactam and clindamycin is the recommended treatment for severe GAS disease (59). Addi-tionally, though tetracycline is not used in the treatment of GAS disease, the susceptibility of GAS to tetracycline seems to be highly variable between countries (26,32,38).…”

Section: Active Surveillance Of Invasive Group a Streptococcal (Gas) mentioning

confidence: 99%

Clinical and Epidemiological Aspects of Invasive Streptococcus pyogenes Infections in Denmark during 2003 and 2004

et al. 2008

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Active surveillance of invasive group A streptococcal (GAS) infections was conducted in Denmark during2003 and 2004 as a part of the Strep-EURO initiative. The main objective was to improve understanding of the epidemiology of invasive GAS disease in Denmark. During the 2 years, 278 cases were reported, corresponding to a mean annual incidence of 2.6 cases per 100,000 inhabitants. The vast majority of isolates, 253 (91%), were from blood, with the remaining 25 (9%) being from cerebrospinal fluid, joints, or other normally sterile sites. The mean case fatality rate (CFR) was 20%, with the rate being higher in patients more than 70 years of age (36.5%). For streptococcal toxic shock syndrome (STSS) and necrotizing fasciitis the CFRs were 53% and 25%, respectively. Out of 16 T types recorded, three predominated: T28 (23%), T1 (22%), and the cluster T3/13/B3264 (14%). Among 29 different emm types, emm28 and emm1 accounted for 51% of strains, followed by emm3 (11%), emm89 (7%), and emm12 (5.5%). Low resistance rates were detected for macrolide-lincosamide-streptogramin B (MLS B ) antibiotics (3%) and tetracycline (8%); two isolates exhibited coresistance to tetracycline and macrolides. Of nine pyrogenic exotoxin (superantigen) genes examined, speA and speC were identified in 58% and 40% of the strains, respectively; either of the genes was present in all strains causing STSS. Most strains harbored speG (99%). ssa was present in 14% of the isolates only. In Denmark, as in comparable countries, GAS invasive disease shows a sustained, high endemicity, with involvement of both established and emerging streptococcal emm and T types.

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Section: Active Surveillance Of Invasive Group a Streptococcal (Gas) mentioning

confidence: 99%

Clinical and Epidemiological Aspects of Invasive Streptococcus pyogenes Infections in Denmark during 2003 and 2004

et al. 2008

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“…is the most extensively studied antibiotic resistance gene in terms of molecular ecology 9) and has been found among numerous species of Gram-negative and Gram-positive bacteria including not only human pathogens 6,20,22,38) but also bacteria obtained from several terrestrial environments associated with human activity 9) such as in agriculture 33) , groundwater 8) , and daily diets 14,16,26,39) . Tet(M) is often accompanied by mobile elements such as plasmids and transposons, such associations contributing to the wide distribution of this gene in bacterial genera obtained from different environments 9) .…”

mentioning

confidence: 99%

Distribution of Tetracycline Resistance Gene, tet(M), in Gram-Positive and Gram-Negative Bacteria Isolated from Sediment and Seawater at a Coastal Aquaculture Site in Japan

2007

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We found increased numbers of oxytetracycline (OTC)-resistant bacteria in sediment and seawater around a marine aquaculture site after OTC therapy. Samples were collected at an aquaculture site along the coast of the Seto Inland Sea, Japan in 2004. In April, the percentage of bacteria resistant to 60 µg mL −1 OTC in the surface sediment was 6.8%-20.0%. The percentages increased during OTC therapy in the summer reaching 53.3%-60.7% in September. Ninety-two days after drug cessation, the percentages decreased to below 22.9%. Tet(M)-positive bacteria were detected in the sediment and seawater samples. Tet(M) was evident in both Gram-positive and Gram-negative bacteria from various genera, and was newly identified in Paenibacillus, Sporosarcina, Shewanella, and Pseudoalteromonas. The dominant tet(M)-positive isolates were strains of Vibrio suggesting that this genus is an important reservoir for tet(M) in the marine environment. Two different alleles were found, tet(M)-A and tet(M)-B, each in isolates from five genera. The data suggests drug therapy used in the aquaculture acted as a selective pressure promoting increased numbers of resistant bacteria.

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“…In Turkey, ERY resistance was observed in 2.7% of isolates (this low rate being ascribed to the limited use of ERY, especially for streptococcal pharyngitis), whilst 22.7% were resistant to TET [42]. Although TET resistance in other bacteria has been studied extensively [43], less is known about the resistance mechanism in GAS [5,[17][18][19][20][21][22][23]. According to current knowledge, the mechanisms of resistance are mainly associated with the presence of the tet(M) or tet(O) genes, which are related to transposon elements.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, erythromycin (ERY) resistance in GAS has been the subject of many reports in recent years [4,[6][7][8][9][10][11][12][13][14][15][16]. In contrast, less information is available on the characteristics of TET resistance mechanisms [5,[17][18][19][20][21][22][23] despite the fact that TET resistance has been known for decades [24] and has been recorded in upwards of 50% of isolates [21]. Variation in the prevalence of resistance to these antibiotics has been attributed to clonality of resistant isolates and to particular practices of antibiotic use in different countries [21,[25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Macrolide, lincosamide and tetracycline susceptibility and emm characterisation of invasive Streptococcus pyogenes isolates in Israel

Nir-Paz¹,

Block²,

Shasha³

et al. 2006

International Journal of Antimicrobial Agents

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Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11

Cited by 5 publications

References 18 publications

Clinical and Epidemiological Aspects of Invasive Streptococcus pyogenes Infections in Denmark during 2003 and 2004

Clinical and Epidemiological Aspects of Invasive Streptococcus pyogenes Infections in Denmark during 2003 and 2004

Distribution of Tetracycline Resistance Gene, tet(M), in Gram-Positive and Gram-Negative Bacteria Isolated from Sediment and Seawater at a Coastal Aquaculture Site in Japan

Macrolide, lincosamide and tetracycline susceptibility and emm characterisation of invasive Streptococcus pyogenes isolates in Israel

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