Molecular mechanisms of herbicide-inducible gene expression of tobacco CYP71AH11 metabolizing the herbicide chlorotoluron

Gion, Keiko; Inui, Hideyuki; Takakuma, Kazuyuki; Yamada, Takashi; Kambara, Yumiko; Nakai, Shuichi; Fujiwara, Hiroyuki; Miyamura, Takashi; Imaishi, Hiromasa; Ohkawa, Hideo

doi:10.1016/j.pestbp.2013.12.003

Cited by 15 publications

(15 citation statements)

References 38 publications

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“…Sequencing individuals from all response groups, haven’t reveal any known substitutions associated with resistance to ALS inhibitors. Similar cases of non-target site resistance to ALS inhibitors have previously been reported, and there is evidence that the involvement of a single gene encoding for cytochrome P450 enzymes can endow this resistance (Yamada et al, 2000; Gion et al, 2014). This type of resistance mechanism can be correlated with the effect of one gene with two alleles, creating three levels of response to the herbicide (sensitive, moderate, and resistant).…”

Section: Discussionsupporting

confidence: 75%

Hyperspectral Technologies for Assessing Seed Germination and Trifloxysulfuron-methyl Response in Amaranthus palmeri (Palmer Amaranth)

et al. 2017

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Weed infestations in agricultural systems constitute a serious challenge to agricultural sustainability and food security worldwide. Amaranthus palmeri S. Watson (Palmer amaranth) is one of the most noxious weeds causing significant yield reductions in various crops. The ability to estimate seed viability and herbicide susceptibility is a key factor in the development of a long-term management strategy, particularly since the misuse of herbicides is driving the evolution of herbicide response in various weed species. The limitations of most herbicide response studies are that they are conducted retrospectively and that they use in vitro destructive methods. Development of a non-destructive method for the prediction of herbicide response could vastly improve the efficacy of herbicide applications and potentially delay the evolution of herbicide resistance. Here, we propose a toolbox based on hyperspectral technologies and data analyses aimed to predict A. palmeri seed germination and response to the herbicide trifloxysulfuron-methyl. Complementary measurement of leaf physiological parameters, namely, photosynthetic rate, stomatal conductence and photosystem II efficiency, was performed to support the spectral analysis. Plant response to the herbicide was compared to image analysis estimates using mean gray value and area fraction variables. Hyperspectral reflectance profiles were used to determine seed germination and to classify herbicide response through examination of plant leaves. Using hyperspectral data, we have successfully distinguished between germinating and non-germinating seeds, hyperspectral classification of seeds showed accuracy of 81.9 and 76.4%, respectively. Sensitive and resistant plants were identified with high degrees of accuracy (88.5 and 90.9%, respectively) from leaf hyperspectral reflectance profiles acquired prior to herbicide application. A correlation between leaf physiological parameters and herbicide response (sensitivity/resistance) was also demonstrated. We demonstrated that hyperspectral reflectance analyses can provide reliable information about seed germination and levels of susceptibility in A. palmeri. The use of reflectance-based analyses can help to better understand the invasiveness of A. palmeri, and thus facilitate the development of targeted control methods. It also has enormous potential for impacting environmental management in that it can be used to prevent ineffective herbicide applications. It also has potential for use in mapping tempo-spatial population dynamics in agro-ecological landscapes.

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Section: Discussionsupporting

confidence: 75%

Hyperspectral Technologies for Assessing Seed Germination and Trifloxysulfuron-methyl Response in Amaranthus palmeri (Palmer Amaranth)

et al. 2017

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“…cDNA microarray analysis indicated that a full‐length cDNA collection contains 49 different P450 genes, which are induced by both abiotic and biotic stresses, but does not contain the P450 species listed in Table . On the other hand, CYP71AH11 metabolising the herbicide chlorotoluron has been induced in tobacco plants by treatment with the herbicides 2,4‐D and bromoxynil, probably owing to the generation of reactive oxygen species . In addition, 2,4‐D has enhanced the metabolism of the herbicide diclofop‐methyl in L. rigidum and protected the susceptible biotype against the herbicide .…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, CYP71AH11 metabolising the herbicide chlorotoluron has been induced in tobacco plants by treatment with the herbicides 2,4-D and bromoxynil, probably owing to the generation of reactive oxygen species. 23 In addition, 2,4-D has enhanced the metabolism of the herbicide diclofop-methyl in L. rigidum and protected the susceptible biotype against the herbicide. 24 Therefore, 2,4-D and bromoxynil seem to be useful for enhancement of herbicide tolerance and selectivity.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the results listed in Table , the CYP71 family was mainly involved in metabolism of phenylurea and sulfonylurea herbicides, although CYP76B1, CYP81B1 and CYP81B2 also metabolised phenylurea herbicides. It was found that tobacco CYP71AH11 induced by 2,4‐D was more actively induced by the herbicide bromoxynil . Han et al .…”

Section: Metabolism Of Herbicides In Plant P450 Speciesmentioning

confidence: 99%

“…It was found that tobacco CYP71AH11 induced by 2,4-D was more actively induced by the herbicide bromoxynil. 23 Han et al 24 reported that 2,4-D protected L. rigidum against the herbicide diclofop-methyl owing to enhanced herbicide metabolism. Thus, both 2,4-D and bromoxynil seem to be useful for enhancement of herbicide tolerance and selectivity.…”

Section: Metabolism Of Herbicides In Plant P450 Speciesmentioning

confidence: 99%

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Metabolism of agrochemicals and related environmental chemicals based on cytochrome P450s in mammals and plants

Ohkawa

Inui

2014

Pest Management Science

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A yeast gene expression system originally established for mammalian cytochrome P450 monooxygenase cDNAs was applied to functional analysis of a number of mammalian and plant P450 species, including 11 human P450 species (CYP1A1, CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1 and CYP3A4). The human P450 species CYP1A1, CYP1A2, CYP2B6, CYP2C18 and CYP2C19 were identified as P450 species metabolising various agrochemicals and environmental chemicals. CYP2C9 and CYP2E1 specifically metabolised sulfonylurea herbicides and halogenated hydrocarbons respectively. Plant P450 species metabolising phenylurea and sulfonylurea herbicides were also identified mainly as the CYP71 family, although CYP76B1, CYP81B1 and CYP81B2 metabolised phenylurea herbicides. The transgenic plants expressing these mammalian and plant P450 species were applied to herbicide tolerance as well as phytoremediation of agrochemical and environmental chemical residues. The combined use of CYP1A1, CYP2B6 and CYP2C19 belonging to two families and three subfamilies covered a wide variety of herbicide tolerance and phytoremediation of these residues. The use of 2,4-D-and bromoxynil-induced CYP71AH11 in tobacco seemed to enhance herbicide tolerance and selectivity.

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Enhanced metabolic detoxification is associated with fluroxypyr resistance in Bassia scoparia

Todd,

Patterson,

Westra

et al. 2024

Plant Direct

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Auxin‐mimic herbicides chemically mimic the phytohormone indole‐3‐acetic‐acid (IAA). Within the auxin‐mimic herbicide class, the herbicide fluroxypyr has been extensively used to control kochia (Bassia scoparia). A 2014 field survey for herbicide resistance in kochia populations across Colorado identified a putative fluroxypyr‐resistant (Flur‐R) population that was assessed for response to fluroxypyr and dicamba (auxin‐mimics), atrazine (photosystem II inhibitor), glyphosate (EPSPS inhibitor), and chlorsulfuron (acetolactate synthase inhibitor). This population was resistant to fluroxypyr and chlorsulfuron but sensitive to glyphosate, atrazine, and dicamba. Subsequent dose‐response studies determined that Flur‐R was 40 times more resistant to fluroxypyr than a susceptible population (J01‐S) collected from the same field survey (LD50 720 and 20 g ae ha−1, respectively). Auxin‐responsive gene expression increased following fluroxypyr treatment in Flur‐R, J01‐S, and in a dicamba‐resistant, fluroxypyr‐susceptible line 9,425 in an RNA‐sequencing experiment. In Flur‐R, several transcripts with molecular functions for conjugation and transport were constitutively higher expressed, such as glutathione S‐transferases (GSTs), UDP‐glucosyl transferase (GT), and ATP binding cassette transporters (ABC transporters). After analyzing metabolic profiles over time, both Flur‐R and J01‐S rapidly converted [14C]‐fluroxypyr ester, the herbicide formulation applied to plants, to [14C]‐fluroxypyr acid, the biologically active form of the herbicide, and three unknown metabolites. The formation and flux of these metabolites were faster in Flur‐R than J01‐S, reducing the concentration of phytotoxic fluroxypyr acid. One unique metabolite was present in Flur‐R that was not present in the J01‐S metabolic profile. Gene sequence variant analysis specifically for auxin receptor and signaling proteins revealed the absence of non‐synonymous mutations affecting auxin signaling and binding in candidate auxin target site genes, further supporting our hypothesis that non‐target site metabolic degradation is contributing to fluroxypyr resistance in Flur‐R.

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Molecular mechanisms of herbicide-inducible gene expression of tobacco CYP71AH11 metabolizing the herbicide chlorotoluron

Cited by 15 publications

References 38 publications

Hyperspectral Technologies for Assessing Seed Germination and Trifloxysulfuron-methyl Response in Amaranthus palmeri (Palmer Amaranth)

Hyperspectral Technologies for Assessing Seed Germination and Trifloxysulfuron-methyl Response in Amaranthus palmeri (Palmer Amaranth)

Metabolism of agrochemicals and related environmental chemicals based on cytochrome P450s in mammals and plants

Enhanced metabolic detoxification is associated with fluroxypyr resistance in Bassia scoparia

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