Molecular mechanisms of apoptosis induction in K562 and KG1a leukemia cells by a water-soluble copper(II) thiosemicarbazone complex

Parsa, Fatemeh Ghorbani; Feizi, Mohammad Ali Hosseinpour; Safaralizadeh, Reza; Hosseini‐Yazdi, Seyed Abolfazl; Mahdavi, Majid

doi:10.1007/s00775-020-01769-0

Cited by 11 publications

(7 citation statements)

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“…with the aid of sulfur atom or nitrogen atom to act as a monodentate ligand or via these two atoms as a bidentate ligand have precipitated some renewed research in recent years . Among them, classically structured half-sandwich ruthenium(II) and osmium(II) TSC complexes have demonstrated antitumor potential; however, few Ir III TSC complexes with appropriate antitumor potential were reported . Then, in this study, a series of TSC derivatives were condensed with formoyl-substituted triphenylamine (TPA) to construct the Schiff base pro-ligands, then coordinated with iridium and obtained four half-sandwiched structure Ir III TSC complexes, Figure .…”

Section: Introductionmentioning

confidence: 99%

“…23 Among them, classically structured half-sandwich ruthenium(II) and osmium(II) TSC complexes have demonstrated antitumor potential; however, few Ir III TSC complexes with appropriate antitumor potential were reported. 24 Then, in this study, a series of TSC derivatives were condensed with formoyl-substituted triphenylamine (TPA) to construct the Schiff base pro-ligands, then coordinated with iridium and obtained four half-sandwiched structure Ir III TSC complexes, Figure 1. 25 The unique structure of TSC derivatives and the appropriate ion radius for the central Ir III ion resulted in the unique half-sandwich structure of these complexes, TSC Schiff bases coordinated with the central Ir III ion through the "enol" configuration and led to the dimeric structure through sulfur bridge bonds (Ir−S−Ir) without the leaving group (Cl).…”

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo of Triphenylamine-Appended Fluorescent Half-Sandwich Iridium(III) Thiosemicarbazones Antitumor Complexes

et al. 2021

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Half-sandwiched structure iridium(III) complexes appear to be an attractive organometallic antitumor agents in recent years. Here, four triphenylamine-modified fluorescent half-sandwich iridium(III) thiosemicarbazone (TSC) antitumor complexes were developed. Because of the “enol” configuration of the TSC ligands, these complexes formed a unique dimeric configuration. Aided by the appropriate fluorescence properties, studies found that complexes could enter tumor cells in an energy-dependent mode, accumulate in lysosomes, and result in the damage of lysosome integrity. Complexes could block the cell cycle, improve the levels of intrastitial reactive oxygen species, and lead to apoptosis, which followed an antitumor mechanism of oxidation. Compared with cisplatin, the antitumor potential in vivo and vitro confirmed that Ir4 could effectively inhibit tumor growth. Meanwhile, Ir4 could avoid detectable side effects in the experiments of safety evaluation. Above all, half-sandwich iridium(III) TSC complexes are expected to be an encouraging candidate for the treatment of malignant tumors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo of Triphenylamine-Appended Fluorescent Half-Sandwich Iridium(III) Thiosemicarbazones Antitumor Complexes

et al. 2021

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“…Parsa et al used flow cytometry to study the effect of Cu-thiosemicarbazide (Cu-TSC) as a leukemia chemotherapeutic agent on K562 cell apoptosis. 2 Xin et al used immunohistochemistry to study the effect of adenoma protein on the proliferation of human leukemia k562 cells. 3 However, the flow cytometry can identify cells only from a small part of the components of cells, and fluorescence microscopy can identify cells only from the structure of cells after the fluorescence staining, rather than detecting the component differences of tumor cells at a molecular level.…”

Section: ■ Introductionmentioning

confidence: 99%

Detection of K562 Leukemia Cells in Different States Using a Graphene-SERS Platform

Jiang

Niu

et al. 2021

ACS Appl. Nano Mater.

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The detection of intact cells in their original state is of great importance for early diagnosis of disease. In this work, we report the detection of living K562 chronic myeloid leukemia cells using an innovative label-free hybrid graphene/gold nanopyramid based surface-enhanced Raman scattering (SERS) trough grid method. The position of the cells can be well controlled by the trough grid so that they can remain unchanged in the liquid at room temperature for up to 2 h. The graphene sheet permits SERS hot spot identification and provides a chemical enhancement for the biological constituents. Different states of K562 cells were thus detected. Two types of SERS signals of K562 cells can be clearly distinguished using principal component analysis (PCA), corresponding to two different states of the K562 cell, namely, the living state and dead state. And their SERS signal ratio severely varies as a function of the storage time. PCA analysis shows that, within 2 h after taking K562 cells out of the incubator, living cells account for more than 60% of the total cells. The SERS signal variation has been attributed to an increased number of dead cells suspended in the cell culture fluid. We demonstrate a proof-of-concept study of the clarification of the state variation, from the molecular level, of the K562 cells as a function of time using a graphene-SERS platform. This method is of great potential for leukemia diagnosis and on the development of leukemia drugs.

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“…Jorge AndrésSolís-Ruiz et al reported increased DNA damage upon irradiation, and caspase 3-dependent apoptosis induced by polypyridyl ruthenium complexes [10]. Fatemeh Ghorbani Parsa et al, reported cell apoptosis induction in K562 and KG1a leukemia cells by water-soluble copper(II) thiosemicarbazone complex [11]. Nesihan Tekin et al reported caspase 3 and Bax genes dependent cell apoptosis induced by Pd(II) complexes [12].…”

Section: Introductionmentioning

confidence: 99%

Mechanistic insight of cell anti-proliferative activity of fluoroquinolone drug-based Cu(II) complexes

et al. 2021

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Pefloxacin-based mixed ligand Cu(II) complexes with substituted isatin of type [Cu(Isatin)(Pefloxacin)Cl] were synthesized, and characterized by EPR, mass, FT-IR, electronic spectrometry, metal content, magnetic moment, and conductance measurement. The g factors g ∥ > g ⟂ > 2.0023 observed in EPR suggest a square-pyramidal environment of ligands around the copper metal. The compounds were screened for diverse biological activities. The compounds inhibit efficiently the cell proliferation of HCT 116 cancer cells. To take the insight of anticancer activity mechanism, we investigated compound-1 for further cellular assay-based biological activities like trypan blue assay, cell morphological alteration assay, colony formation assay, cell apoptosis, and cell necrosis assay. The compound-1 induced distinct morphological alteration in cells, inhibits cell viability, decreases % plating efficiency, and decreases the clonogenic ability of the HCT 116 cells. The cell death mechanism was confirmed by annexin V-FITC / PI assay and LDH release assay. The positive annexin V/PI stained cells in presence of compound-1 and the absence of a significant amount of lactate dehydrogenase suggest cell apoptosis mechanism for anticancer activity of compounds. We also screened compounds for in vitro antibacterial and cytotoxic activities.

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Molecular mechanisms of apoptosis induction in K562 and KG1a leukemia cells by a water-soluble copper(II) thiosemicarbazone complex

Cited by 11 publications

References 45 publications

In Vitro and In Vivo of Triphenylamine-Appended Fluorescent Half-Sandwich Iridium(III) Thiosemicarbazones Antitumor Complexes

In Vitro and In Vivo of Triphenylamine-Appended Fluorescent Half-Sandwich Iridium(III) Thiosemicarbazones Antitumor Complexes

Detection of K562 Leukemia Cells in Different States Using a Graphene-SERS Platform

Mechanistic insight of cell anti-proliferative activity of fluoroquinolone drug-based Cu(II) complexes

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