2012
DOI: 10.1007/s12551-012-0078-7
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Molecular mechanisms of acetylcholine receptor–lipid interactions: from model membranes to human biology

Abstract: Lipids are potent modulators of the Torpedo nicotinic acetylcholine receptor. Lipids influence nicotinic receptor function by allosteric mechanisms, stabilizing varying proportions of pre-existing resting, open, desensitized, and uncoupled conformations. Recent structures reveal that lipids could alter function by modulating transmembrane α-helix/α-helix packing, which in turn could alter the conformation of the allosteric interface that links the agonist-binding and transmembrane pore domains-this interface i… Show more

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Cited by 18 publications
(15 citation statements)
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“…When a membrane protein is purified in its native membrane or reconstituted into a model bilayer, as is often necessary in order to maintain activity, it is difficult to distinguish whether functional effects of different lipids result from specific protein-lipid interactions or from physical forces associated with nonspecific bilayer interactions. Thus, the different types of protein-lipid interaction have been a matter of extensive debate for channels (20,21), transporters (22), G-protein-coupled receptors (23), and P-type ATPases (SI Appendix, Supplementary Discussion). By contrast, when the membrane protein is purified in soluble mixed protein-lipid-detergent micelles in the absence of a lipid bilayer, the analysis is greatly simplified.…”
Section: Discussionmentioning
confidence: 99%
“…When a membrane protein is purified in its native membrane or reconstituted into a model bilayer, as is often necessary in order to maintain activity, it is difficult to distinguish whether functional effects of different lipids result from specific protein-lipid interactions or from physical forces associated with nonspecific bilayer interactions. Thus, the different types of protein-lipid interaction have been a matter of extensive debate for channels (20,21), transporters (22), G-protein-coupled receptors (23), and P-type ATPases (SI Appendix, Supplementary Discussion). By contrast, when the membrane protein is purified in soluble mixed protein-lipid-detergent micelles in the absence of a lipid bilayer, the analysis is greatly simplified.…”
Section: Discussionmentioning
confidence: 99%
“…Neuronal nAChRs traffic to the cell surface via lipid rafts. Disruption of these rafts leads to both altered cell surface exposure and altered nAChR function 12,48,49 . Integral membrane proteins with shorter transmembrane α-helices tend to remain in intracellular membrane compartments, possibly because they are a better hydrophobic match for the thinner intracellular membranes 33,50 .…”
Section: Discussionmentioning
confidence: 99%
“…In favorable environments, either direct lipid binding or altered bulk membrane properties could influence transmembrane α-helix packing, thus awakening previously uncoupled receptors 12 .…”
mentioning
confidence: 99%
“…Neuronal nAChRs traffic to the cell surface via lipid rafts, and disruption of these rafts leads to both altered cell surface exposure and altered nAChR function (Baenziger and daCosta, 2012;Baier et al, 2010;Borroni and Barrantes, 2011). Integral membrane proteins with shorter transmembrane a-helices tend to remain in intracellular membrane compartments, possibly because they hydrophobically match the thinner intracellular membranes more favorably (Bretscher and Munro, 1993;Lundbaek et al, 2003).…”
Section: M4 and The Folding And Trafficking Of Nachrsmentioning
confidence: 99%