Molecular Mechanisms for Coping with Al Toxicity in Plants

Zhang, Xiang; Long, Yan; Huang, Jingjing; Xia, Jixing

doi:10.3390/ijms20071551

Cited by 86 publications

(62 citation statements)

References 127 publications

(184 reference statements)

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“…acetic acid). A very recent study however identified a bidirectional aluminum-malate transporter, which may be also involved in root malate uptake 42 . Results here presented, reveal that tomato plants are able to take up citrate from the uptake solution (Fig.…”

Section: Discussionmentioning

confidence: 99%

Phosphorus deficiency changes carbon isotope fractionation and triggers exudate reacquisition in tomato plants

Tiziani

Pii

Celletti

et al. 2020

Sci Rep

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Plant roots are able to exude vast amounts of metabolites into the rhizosphere in response to phosphorus (P) deficiency. Causing noteworthy costs in terms of energy and carbon (C) for the plants. Therefore, it is suggested that exudates reacquisition by roots could represent an energy saving strategy of plants. This study aimed at investigating the effect of P deficiency on the ability of hydroponically grown tomato plants to re-acquire specific compounds generally present in root exudates by using 13C-labelled molecules. Results showed that P deficient tomato plants were able to take up citrate (+ 37%) and malate (+ 37%), particularly when compared to controls. While glycine (+ 42%) and fructose (+ 49%) uptake was enhanced in P shortage, glucose acquisition was not affected by the nutritional status. Unexpectedly, results also showed that P deficiency leads to a 13C enrichment in both tomato roots and shoots over time (shoots—+ 2.66‰, roots—+ 2.64‰, compared to control plants), probably due to stomata closure triggered by P deficiency. These findings highlight that tomato plants are able to take up a wide range of metabolites belonging to root exudates, thus maximizing C trade off. This trait is particularly evident when plants grew in P deficiency.

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Section: Discussionmentioning

confidence: 99%

Phosphorus deficiency changes carbon isotope fractionation and triggers exudate reacquisition in tomato plants

Tiziani

Pii

Celletti

et al. 2020

Sci Rep

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“…Homologous MATE proteins with similar citrate transport functions have been identified from wheat 12 , maize 13 , sorghum 14 , rice 15 , and Arabidopsis 16 . In addition to the citrate transporter MATE, another Al-activated malate transporter (ALMT) has also been reported in many plants and is associated with the malate-mediated Al detoxification 17,18 . Genetic studies on the Al-tolerance mechanism in grain legumes are still very limited.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and transcriptional analyses of MATE transporter genes in root tips of wildCicerspp. under aluminium stress

Zhang¹,

Weir²,

Wei³

et al. 2020

Preprint

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26Chickpea is an economically important legume crop with high nutritional value in human diets. 27 Aluminium-toxicity poses a significant challenge for the yield improvement of this increasingly 28 popular crop in acidic soils. The wild progenitors of chickpea may provide a more diverse gene pool 29 for Al-tolerance in chickpea breeding. However, the genetic basis of Al-tolerance in chickpea and its 30 wild relatives remains largely unknown. Here, we assessed the Al-tolerance of six selected wild 31 Cicer accessions by measuring the root elongation in solution culture under control (0 µM Al 3+ ) and 32 Al-treatment (30 µM Al 3+ ) conditions. Al-treatment significantly reduced the root elongation in all 33 target lines compared to the control condition after 2-day's growth. However, the relative 34 reduction of root elongation in different lines varied greatly: 3 lines still retained significant root 35 growth under Al-treatment, whilst another 2 lines displayed no root growth at all. We performed 36 genome-wide identification of multidrug and toxic compound extrusion (MATE) encoding genes in 37 the Cicer genome. A total of 56 annotated MATE genes were identified, which divided into 4 major 38 phylogeny groups (G1-4). Four homologues to lupin LaMATE (> 50% aa identity; named CaMATE1-39 4) were clustered with previously characterised MATEs related to Al-tolerance in various other 40 plants. qRT-PCR showed that CaMATE2 transcription in root tips was significantly up-regulated 41 upon Al-treatment in all target lines, whilst CaMATE1 was up-regulated in all lines except Bari2_074 42 and Deste_064, which coincided with the lines displaying no root growth under Al-treatment. 43 Transcriptional profiling in five Cicer tissues revealed that CaMATE1 is specifically transcribed in the 44 root tissue, further supporting its role in Al-detoxification in roots. This first identification of MATE-45 encoding genes associated with Al-tolerance in Cicer paves the ways for future functional 46 characterization of MATE genes in Cicer spp., and to facilitate future design of gene-specific 47 markers for Al-tolerant line selection in chickpea breeding programs.48

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“…Plants utilize two different mechanisms to develop Al tolerance: the Al exclusion to prevent/reduce the entry of the ions into root-tip cells and the ability to tolerate internalized Al 63,64 . Hematoxylin is a dye which forms a complex with Al, and the color intensity of stained root apices can be used as a measurement of the amount of Al accumulated/internalized in root-tip cells 43,44 .…”

Section: Discussionmentioning

confidence: 99%

Al-induced proteomics changes in tomato plants over-expressing a glyoxalase I gene

Thapa

et al. 2020

Hortic Res

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Glyoxalase I (Gly I) is the first enzyme in the glutathionine-dependent glyoxalase pathway for detoxification of methylglyoxal (MG) under stress conditions. Transgenic tomato 'Money Maker' plants overexpressing tomato SlGlyI gene (tomato unigene accession SGN-U582631/Solyc09g082120.3.1) were generated and homozygous lines were obtained after four generations of self-pollination. In this study, SlGlyI-overepxressing line (GlyI), wild type (WT, negative control) and plants transformed with empty vector (ECtr, positive control), were subjected to Al-treatment by growing in Magnavaca's nutrient solution (pH 4.5) supplemented with 20 µM Al 3+ ion activity. After 30 days of treatments, the fresh and dry weight of shoots and roots of plants from Al-treated conditions decreased significantly compared to the non-treated conditions for all the three lines. When compared across the three lines, root fresh and dry weight of GlyI was significant higher than WT and ECtr, whereas there was no difference in shoot tissues. The basal 5 mm root-tips of GlyI plants expressed a significantly higher level of glyoxalase activity under both non-Al-treated and Al-treated conditions compared to the two control lines. Under Al-treated condition, there was a significant increase in MG content in ECtr and WT lines, but not in GlyI line. Quantitative proteomics analysis using tandem mass tags mass spectrometry identified 4080 quantifiable proteins and 201 Al-induced differentially expressed proteins (DEPs) in roottip tissues from GlyI, and 4273 proteins and 230 DEPs from ECtr. The Al-down-regulated DEPs were classified into molecular pathways of gene transcription, RNA splicing and protein biosynthesis in both GlyI and ECtr lines. The Alinduced DEPs in GlyI associated with tolerance to Al 3+ and MG toxicity are involved in callose degradation, cell wall components (xylan acetylation and pectin degradation), oxidative stress (antioxidants) and turnover of Al-damaged epidermal cells, repair of damaged DNA, epigenetics, gene transcription, and protein translation. A protein-protein association network was constructed to aid the selection of proteins in the same pathway but differentially regulated in GlyI or ECtr lines. Proteomics data are available via ProteomeXchange with identifiers PXD009456 under project title '25Dec2017_Suping_XSexp2_ITAG3.2' for SlGlyI-overexpressing tomato plants and PXD009848 under project title '25Dec2017_Suping_XSexp3_ITAG3.2' for positive control ECtr line transformed with empty vector.

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Molecular Mechanisms for Coping with Al Toxicity in Plants

Cited by 86 publications

References 127 publications

Phosphorus deficiency changes carbon isotope fractionation and triggers exudate reacquisition in tomato plants

Phosphorus deficiency changes carbon isotope fractionation and triggers exudate reacquisition in tomato plants

Genome-wide identification and transcriptional analyses of MATE transporter genes in root tips of wildCicerspp. under aluminium stress

Al-induced proteomics changes in tomato plants over-expressing a glyoxalase I gene

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