Molecular mechanisms for ABCA1-mediated cholesterol efflux

Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?

“…Within the large class of transporters whose expression is affected by mycotoxins, we also include the SLC superfamily of afflux transporters [ 95 , 96 ]. Given the effect of AFB1 on mRNA levels of these transporters, we suppose that the mycotoxin negatively influenced steroids, peptides and glucose transport and metabolism [ 97 , 98 , 99 ]. For example, we observed the down-regulation of ABCA1 , SLC15A1 (pept1), SLC2A2 (glut2) and SLC5A1 (sglt1) genes; on the other hand, a restoration of their mRNA levels was noticed in the presence of BEN.…”

Analysis of the orientation of cholesterol in high-density lipoprotein nanodiscs using solid-state NMR

“…Nanoparticles of rHDL were prepared by detergent dialysis 30 from a mixture of palmitoyloleoylphosphatidylcholine (POPC), [2,3,[4][5][6][7][8][9][10][11][12][13] C 3 ]cholesterol and recombinant apoA-I in a molar ratio of 200 : 20 : 2 (these proportions denote the average ratio of lipid, cholesterol and protein per particle). For NMR analysis, the particles were precipitated from aqueous solution by polyethylene glycol (PEG) as described previously.…”

“…The morphological plasticity of HDL influences their lipid-binding properties and metabolism, and the surface curvature of the lipid cargo regulates interactions with cholesterol-handling enzymes. [5][6][7][8] Consequently, information on the dynamics and orientation of cholesterol in HDL is pertinent to its essential role in cholesterol cycling. 4 A wealth of information on the physical properties of cholesterol and its effect on phospholipid bilayers has been gained by using solid-state NMR (ssNMR) spectroscopy with model membrane systems such as multilamellar vesicles (MLVs).…”

“…The morphological plasticity of HDL influences their lipid-binding properties and metabolism, and the surface curvature of the lipid cargo regulates interactions with cholesterol-handling enzymes. 5–8 Consequently, information on the dynamics and orientation of cholesterol in HDL is pertinent to its essential role in cholesterol cycling. 4…”

“…[2,3,[4][5][6][7][8][9][10][11][12][13] C 3 ]cholesterol (25.7 mM) stocks in CHCl 3 were combined in a round-bottom flask to a final molar ratio of POPC: [2,3,4-13 C 3 ]cholesterol of 10:1 (mol/mol). The CHCl 3 was evaporated under a stream of N 2, and the thin film was placed under vacuum for ≥ 4 hours.…”