Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules

Васильева, В. Ю.; Yue, Zuojun; O’Reilly, Nicola; Kitamura, Etsushi; Tanaka, Tomoyuki

doi:10.1083/jcb.201608122

Cited by 19 publications

(31 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We fused BIR1 and MCM21 with the auxin-induced degron tag (bir1-aid and mcm21-aid) and investigated how this double depletion affected cell growth ( Figure 1A). STU1 is an essential gene and stu1-aid was used as a control for viability [17].…”

Section: Coma Facilitates Chromosome Bi-orientation Independently Of mentioning

confidence: 99%

Aurora B-INCENP localization at centromeres/inner kinetochores is essential for chromosome bi-orientation in budding yeast

García-Rodríguez

Kasciukovic

Denninger

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

To promote chromosome bi-orientation, Aurora B kinase weakens and disrupts aberrant kinetochore-MT interaction. It has long been debated how Aurora B halts this action when biorientation is established and tension is applied across sister kinetochores. Pertinent to this debate, it was shown that Bir1 (yeast Survivin), which recruits Ipl1-Sli15 (yeast Aurora B-INCENP) to centromeres, is dispensable for bi-orientation, raising the possibility that Aurora B localization at centromeres is not required for bi-orientation. Here, we show that the COMA inner kinetochore sub-complex physically interacts with Sli15, recruits Ipl1-Sli15 to the inner kinetochore and promotes chromosome bi-orientation, independently of Bir1, in budding yeast. Moreover, using an engineered recruitment of Ipl1-Sli15 to the inner kinetochore when both Bir1 and COMA are defective, we show that localization of Ipl1-Sli15 at centromeres/ inner kinetochores is essential for bi-orientation, refuting the above possibility. Our results give important insight into how Aurora B disrupts kinetochore-MT interaction in a tensiondependent manner, to promote chromosome bi-orientation. (164 words)

show abstract

Section: Coma Facilitates Chromosome Bi-orientation Independently Of mentioning

confidence: 99%

Aurora B-INCENP localization at centromeres/inner kinetochores is essential for chromosome bi-orientation in budding yeast

García-Rodríguez

Kasciukovic

Denninger

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Table 1 lists the parameters used to solve the model. Five parameter values were taken from previous studies (Gay et al, 2012;Gonen et al, 2012;Nannas et al, 2014;O'Toole et al, 1997;Storchova et al, 2006;Vasileva et al, 2017) The other parameters are given in Figure 1C.…”

Section: Discussionmentioning

confidence: 99%

“…A microtubule that comes into the proximity of a kinetochore (KC), a protein complex at the sister chromatids, can attach to the KC and thus establish a link between chromatids and spindle poles (Akiyoshi et al, 2010;Gonen et al, 2012;Hill, 1985;Mitchison and Kirschner, 1985;Volkov et al, 2013). Theoretical studies have quantitatively shown that this process can contribute to spindle assembly (Kalinina et al, 2013;Paul et al, 2009;Vasileva et al, 2017;Wollman et al, 2005). Prior to chromosome separation, all connections between chromatids and the spindle pole must be established, and erroneous KC-microtubule attachments must be corrected for which several mechanisms are proposed (Tubman et al, 2017;Zaytsev and Grishchuk, 2015).…”

Section: Introductionmentioning

confidence: 99%

Chromosome loss rate in cells of different ploidy can be explained by spindle self-organization

Jelenić

Selmecki

Laan

et al. 2017

Preprint

View full text Add to dashboard Cite

SummaryThe mitotic spindle segregates chromosomes and minimizes chromosome loss for the specific number of chromosomes present in an organism. In Saccharomyces cerevisiae, for example, haploid and diploid cells are part of the sexual life cycle and have a thousand times lower rate of chromosome loss than tetraploid cells. Currently it is unclear what constrains the number of chromosomes that can be segregated with high fidelity in an organism. Here we developed a mathematical model to study if different rates of chromosome loss in cells with different ploidy can arise from changes in (1) spindle dynamics and (2) a maximum duration of mitotic arrest, after which cells enter anaphase. Our model reveals how small increases in spindle assembly time can result in exponential differences in rate of chromosomes loss between cells of increasing ploidy and predicts the maximum duration of mitotic arrest.

show abstract

“…4 ; Kitamura et al, 2007 ). The simulation was previously developed ( Gandhi et al, 2011 ; Vasileva et al, 2017 ), but several modifications were introduced in this study. The values of the majority of parameters were determined, based on previous studies ( Gandhi et al, 2011 ; Kalinina et al, 2013 ; Kitamura et al, 2007 , 2010 ; Tanaka et al, 2007 , 2005 ), and some unknown parameter values were determined in the current study ( ).…”

Section: Methodsmentioning

confidence: 99%

Mechanisms mitigating problems associated with multiple kinetochores on one microtubule in early mitosis

Yue

Komoto

Pasquali

et al. 2017

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

Proper chromosome segregation in mitosis relies on correct kinetochore interaction with spindle microtubules. In early mitosis, each kinetochore usually interacts with the lateral side of each microtubule and is subsequently tethered at the microtubule end. However, since eukaryotic cells carry multiple chromosomes, multiple kinetochores could occasionally interact with a single microtubule. The consequence of this is unknown. Here, we find that, although two kinetochores (two pairs of sister kinetochores) can interact with the lateral side of one microtubule, only one kinetochore can form a sustained attachment to the microtubule end in budding yeast (Saccharomyces cerevisiae). This leads to detachment of the other kinetochore from the microtubule end (or a location in its proximity). Intriguingly, in this context, kinetochore sliding along a microtubule towards a spindle pole delays and diminishes discernible kinetochore detachment. This effect expedites collection of the entire set of kinetochores to a spindle pole. We propose that cells are equipped with the kinetochore-sliding mechanism to mitigate problems associated with multiple kinetochores on one microtubule in early mitosis.

show abstract

Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules

Cited by 19 publications

References 56 publications

Aurora B-INCENP localization at centromeres/inner kinetochores is essential for chromosome bi-orientation in budding yeast

Aurora B-INCENP localization at centromeres/inner kinetochores is essential for chromosome bi-orientation in budding yeast

Chromosome loss rate in cells of different ploidy can be explained by spindle self-organization

Mechanisms mitigating problems associated with multiple kinetochores on one microtubule in early mitosis

Contact Info

Product

Resources

About