Molecular mechanism of tRNA binding by the Escherichia coli N7 guanosine methyltransferase TrmB

Schultz, SarahK.; Meadows, Kieran; Kothe, Ute

doi:10.1016/j.jbc.2023.104612

Cited by 6 publications

(2 citation statements)

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“…As expected, the trmB∆ mutant strain presents an H 2 O 2 -sensitive phenotype, and the survival rate of trmB∆ cells exposed to 20 mM H 2 O 2 was found to be only 10% of that of the wildtype strain [158]. Interestingly, similar H 2 O 2 -sensitive phenotypes have been reported for trmB or trm8 mutants in E. coli [160], in Acinetobacter baumannii [161] and in the fungus Colletotrichum lagenarium [162], demonstrating the widespread implication of the m 7 G46 tRNA core modification in the response to oxidative stress. Although tRNA modification genes may be directly involved in bacterial antibiotic resistance-e.g., trmD introducing m 1 G37 in the anticodon loop (reviewed in [167])-, tRNA core modifications have not been shown to confer resistance to antibiotics.…”

Section: G46 Is Implicated In the Response To Oxidative Stresssupporting

confidence: 83%

“…As expected, the trmB

mutant strain presents an

-sensitive phenotype, and the survival rate of trmB

cells exposed to 20 mM

was found to be only 10% of that of the wild-type strain [ 158 ]. Interestingly, similar

-sensitive phenotypes have been reported for trmB or trm8 mutants in E. coli [ 160 ], in Acinetobacter baumannii [ 161 ] and in the fungus Colletotrichum lagenarium [ 162 ], demonstrating the widespread implication of the

G46 tRNA core modification in the response to oxidative stress.…”

Section: Trna Core Modifications and Their Impact On Translation And ...supporting

confidence: 65%

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Beyond the Anticodon: tRNA Core Modifications and Their Impact on Structure, Translation and Stress Adaptation

Yared,

Marcelot,

Barraud

2024

Genes

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Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional chemical modifications. Approximately 100 different modifications have been identified in tRNAs, and each tRNA typically contains 5–15 modifications that are incorporated at specific sites along the tRNA sequence. These modifications may be classified into two groups according to their position in the three-dimensional tRNA structure, i.e., modifications in the tRNA core and modifications in the anticodon-loop (ACL) region. Since many modified nucleotides in the tRNA core are involved in the formation of tertiary interactions implicated in tRNA folding, these modifications are key to tRNA stability and resistance to RNA decay pathways. In comparison to the extensively studied ACL modifications, tRNA core modifications have generally received less attention, although they have been shown to play important roles beyond tRNA stability. Here, we review and place in perspective selected data on tRNA core modifications. We present their impact on tRNA structure and stability and report how these changes manifest themselves at the functional level in translation, fitness and stress adaptation.

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