Molecular Mechanism of Salt Tolerance for Genetic Diversity Analysed in Association with Na+/K+ Ratio through SSR Markers in Rice (Oryza sativa L.)

Senguttuvel, P.; Raveendran, M.; Vijayalakshmi, C.; Thiyagarajan, K.; Bapu, J.R. Kannan; Viraktamath, B. C.

doi:10.3923/ijar.2010.708.719

Cited by 10 publications

(5 citation statements)

References 11 publications

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“…The markers with PIC values of ≥0.5 are highly informative and widely useful in discriminating the polymorphism rate of a marker at a particular locus (De Woody et al, 1995). The highest genetic distance (≥0.86) observed between the wild salt-tolerant and all other salt-susceptible genotypes revealed that the analyzed genotypes harbored suff icient genetic variations due to differences in their origin, ecotype, and speciation (Ram et al, 2007;Senguttuvel et al, 2010). Genotypes showing genetic distance values of ≥0.70 can be considered as highly genetic divergent for the trait of interest that can facilitate the selection of parental lines for breeding salttolerant variants.…”

Section: Discussionmentioning

confidence: 99%

Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek)

Sehrawat

Bhat

Kaga

et al. 2014

Span. j. agric. res.

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Thirty eight novel microsatellite markers (SSRs) specific to candidate genes involved in salt tolerance were developed for detection of genetic variations in 12 mungbean genotypes variably adapted to salt stress. A 100 out of 124 putative alleles were found polymorphic between wild and cultivated genotypes (inter-specific, 80.65%), 65 were within cultivars of mungbean (intra-specific, 52.42%) and 52 were within wild genotypes (inter-specific, 41.94%). The polymorphism varied from 86.84% to 100%, while the number of polymorphic alleles ranged from 1 to 4 with an average value of 2.63 per locus. The polymorphism information content (PIC) values ranged from 0.326 to 0.875 with an average value of 0.671, which shows their effectiveness in genetic analysis. Cluster analysis resulted in the distribution of salt tolerant and susceptible genotypes in separate groups which revealed the presence of inherent variations among mungbean cultivars. These variations were explored effectively for SSR markers studies. The developed SSR markers may help along with already available markers to execute further research on mungbean. The markers may be coupled with specific loci linked with salt tolerance. The developed markers will help to identify the QTLs (quantitative trait loci) or other important genes. These markers can also be utilized for testing the purity of hybrids or diversity assessment of Vigna species for important agronomic traits.

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Section: Discussionmentioning

confidence: 99%

Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek)

Sehrawat

Bhat

Kaga

et al. 2014

Span. j. agric. res.

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“…In case of plant height, Talmugra, Polai, Marishal, Raspanjar and Kamini were taller than Pokkali. Additionally, in selection process of tolerant genotypes, rice lines with more genetic diversity is essential to develop a good variety (Senguttuvel et al, 2010). We found rice genotypes selected as highly tolerant (Marishal and Kamini) have distant genetic relationship with Pokkali as well as other tolerant genotypes.…”

Section: Discussionmentioning

confidence: 87%

Identification of New Alleles in Salt Tolerant Rice Germplasm Lines through Phenotypic and Genotypic Screening

Bharathkumar¹,

Jena²,

Kumar³

et al. 2016

IJAB

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The present study investigated eight rice lines (Rupsal, Nagalmutha, Polai, Ravana, Marishal, Talmugra, Kamini and Raspanjar) collected from coastal region of eastern India for salinity tolerance through phenotypic and genotypic screening. Among these, three rice lines as highly tolerant (Talmugra, Marishal and Kamini), three tolerant (Rupsal, Polai and Raspanjar) and two moderately tolerant (Ravana and Nagalmutha) to salt stress were identified in phenotypic screening. Pokkali was categorized as tolerant under salinity condition (12 EC dS m -1

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“…Significantly variable DNA quantities were resulted when Senguttuvel et al,(2010) 1g of ground caryopses of the three rice varieties were subjected to DNA extraction using CTAB extraction method, modified CTAB extraction method, Promega kit and Qiagen kit. The highest mean DNA yield was obtained for the rice variety Bg 250 when extracted using modified CTAB method (7586.3 µg/ml).…”

Section: Yield and Purity Of Extracted Dnamentioning

confidence: 99%

Identification of a robust protocol for isolating PCR amenable DNA from caryopses to employ DNA fingerprinting to claim the plant breeders’ rights and varietal ownership of the exporting rice bulks

Ananda¹,

Wijesundara²,

Udawela³

et al. 2018

Ceylon J. Sci.

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Rice Research and Development Institute (RRDI) at Bathalagoda and its' substations are solely conducting rice breeding in Sri Lanka. Recently, RRDI has identified five exportable rice varieties/lines. As rice export in Sri Lanka is undertaken by the private sector, the RRDI does not receive any foreign revenues or credit for breeding rice genotypes. Therefore, RRDI currently is in need of a reliable protocol to claim the Plant Breeders' Rights (PBR) of these exportable genotypes. The first step of establishing such a protocol based on DNA fingerprinting is depending on the ability to extract good quality DNA from rice caryopses. Therefore, the present study compared the suitability of commonly available DNA extraction methods to purify DNA from rice caryopsis. Three rice genotypes; Bg 250 and At 353 (varieties for local consumption) and Bw-Bs-1-2-31 (with export potential) were selected. Three parboiled rice samples each from long-and short-grain groups were also selected. The DNA extraction, quantification and purity assessment were carried out using four methods viz., CTAB, modified CTAB, Promega kit and Qiagen kit. The extracted DNA was subjected to simplex and multiplex PCR using four rice specific DNA markers. Although positive PCR bands were obtained for all four methods, the modified CTAB method yielded the significantly highest DNA yield (6196.67µg/ml) compared to other methods. Thus, RRDI can employ modified CTAB method in the protocol to claim its PBR and rice varietal ownerships.

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Molecular Mechanism of Salt Tolerance for Genetic Diversity Analysed in Association with Na+/K+ Ratio through SSR Markers in Rice (Oryza sativa L.)

Cited by 10 publications

References 11 publications

Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek)

Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek)

Identification of New Alleles in Salt Tolerant Rice Germplasm Lines through Phenotypic and Genotypic Screening

Identification of a robust protocol for isolating PCR amenable DNA from caryopses to employ DNA fingerprinting to claim the plant breeders’ rights and varietal ownership of the exporting rice bulks

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