Molecular mechanism of modulating miR482b level in tomato with botrytis cinerea infection

Wu, Fuyong; Xu, Jinfeng; Gao, Tengteng; Huang, Diao; Jin, Weibo

doi:10.1186/s12870-021-03203-2

Cited by 12 publications

(5 citation statements)

References 61 publications

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“…Notably, pathogenic infections were effectively suppressed in potato [ 31 ], tomato [ 32 ], and cotton [ 33 ] plants through the inhibition of miR482. We found that the expression of stu-miR482a-5p and stu-miR482a-3p was up-regulated with an increase in N application under both types of N stress, which could indicate that miR482a-5p is sensitive to different N treatments.…”

Section: Discussionmentioning

confidence: 99%

Screening of differentially expressed microRNAs and target genes in two potato varieties under nitrogen stress

et al. 2022

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Background A reasonable supply of nitrogen (N) fertilizer is essential for obtaining high-quality, high-level, and stable potato yields, and an improvement in the N utilization efficiency can effectively reduce N fertilizer use. It is important to use accurate, straightforward, and efficient transgenic breeding techniques for the identification of genes that can improve nitrogen use efficiency, thus enabling us to achieve the ultimate goal of breeding N-efficient potato varieties. In recent years, some of the mechanisms of miRNAs have been elucidated via the analysis of the correlation between the expression levels of potato miRNA target genes and regulated genes under conditions of stress, but the role of miRNAs in the inhibition/expression of key genes regulating N metabolism under N stress is still unclear. Our study aimed to identify the role played by specific enzymes and miRNAs in the responses of plants to N stress. Results The roots and leaves of the N-efficient potato variety, Yanshu4 ("Y"), and N-inefficient potato variety, Atlantic ("D"), were collected at the seedling and budding stages after they were exposed to different N fertilizer treatments. The miRNAs expressed differentially under the two types of N stress and their corresponding target genes were first predicted using miRNA and degradome analysis. Then, quantitative polymerase chain reaction (qRT-PCR) was performed to verify the expression of differential miRNAs that were closely related to N metabolism. Finally, the shearing relationship between stu-miR396-5p and its target gene StNiR was determined by analyzing luciferase activity levels. The results showed that NiR activity increased significantly with an increase in the applied N levels from the seedling stage to the budding stage, and NiR responded significantly to different N treatments. miRNA sequencing enabled us to predict 48 families with conserved miRNAs that were mainly involved in N metabolism, carbon metabolism, and amino acid biosynthesis. The differences in the expression of the following miRNAs were identified via screening (high expression levels and P < 0.05): stu-miR396-5p, stu-miR408b-3p_R-1, stu-miR3627-3p, stu-miR482a-3p, stu-miR8036-3p, stu-miR482a-5p, stu-miR827-5p, stu-miR156a_L-1, stu-miR827-3p, stu-miR172b-5p, stu-miR6022-p3_7, stu-miR398a-5p, and stu-miR166c-5p_L-3. Degradome analysis showed that most miRNAs had many-to-many relationships with target genes. The main target genes involved in N metabolism were NiR, NiR1, NRT2.5, and NRT2.7. qRT-PCR analysis showed that there were significant differences in the expression levels of stu-miR396-5p, stu-miR8036-3p, and stu-miR482a-3p in the leaves and roots of the Yanshu4 and Atlantic varieties at the seedling and budding stages under conditions that involved no N and excessive N application; the expression of these miRNAs was induced in response to N stress. The correlation between the differential expression of stu-miR396-5p and its corresponding target gene NiR was further verified by determining the luciferase activity level and was found to be strongly negative. Conclusion The activity of NiR was significantly positively correlated with N application from the seedling to the budding stage. Differential miRNAs and target genes showed a many-to-many relationship with each other. The expression of stu-miR396-5p, stu-miR482a-3p, and stu-miR8036-3p in the roots and leaves of the Yanshu4 and Atlantic varieties at the seedling and budding stages was notably different under two types of N stress. Under two types of N stress, stu-miR396-5p was down-regulated in Yanshu4 in the seedling-stage and shoot-stage roots, and up-regulated in seedling-stage roots and shoot-stage leaves; stu-miR482a-3p was up-regulated in the seedling and shoot stages. The expression of stu-miR8036-3p was up-regulated in the leaves and roots at the seedling and budding stages, and down-regulated in roots under both types of N stress. The gene expressing the key enzyme involved in N metabolism, StNiR, and the stu-miR396-5p luciferase assay reporter gene had a strong regulatory relationship with each other. This study provides candidate miRNAs related to nitrogen metabolism and highlights that differential miRNAs play a key role in nitrogen stress in potato, providing insights for future research on miRNAs and their target genes in nitrogen metabolic pathways and breeding nitrogen-efficient potatoes.

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Section: Discussionmentioning

confidence: 99%

Screening of differentially expressed microRNAs and target genes in two potato varieties under nitrogen stress

et al. 2022

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“…In wheat, tae-miR171a was validated to have an obvious response to drought memory by regulating the expression of target genes [ 41 ]. The molecular mechanism of miR482b expression in tomato infected by Botrytis cinerea was revealed [ 42 ]. In plants, miR390 is an ancient family of miRNAs and is conserved among species [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

MIR390 Is Involved in Regulating Anthracnose Resistance in Apple

Shi

Jiang

Zhang

et al. 2022

Plants

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As an important cash crop in China, apple has a good flavor and is rich in nutrients. Fungal attacks have become a major obstacle in apple cultivation. Colletotrichum gloeosporioides is one of the most devastating fungal pathogens in apple. Thus, discovering resistance genes in response to C. gloeosporioides may aid in designing safer control strategies and facilitate the development of apple resistance breeding. A previous study reported that ‘Hanfu’ autotetraploid apple displayed higher C. gloeosporioides resistance than ‘Hanfu’ apple, and the expression level of mdm-MIR390b was significantly upregulated in autotetraploid plants compared to that in ‘Hanfu’ plants, as demonstrated by digital gene expression (DGE) analysis. It is still unclear, however, whether mdm-MIR390b regulates apple anthracnose resistance. Apple MIR390b was transformed into apple ‘GL-3′ plants to identify the functions of mdm-MIR390b in anthracnose resistance. C. gloeosporioides treatment analysis indicated that the overexpression of mdm-MIR390b reduced fungal damage to apple leaves and fruit. Physiology analysis showed that mdm-MIR390b increased C. gloeosporioides resistance by improving superoxide dismutase (SOD) and peroxidase (POD) activity to alleviate the damage caused by O2− and H2O2. Our results demonstrate that mdm-MIR390b can improve apple plants’ anthracnose resistance.

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“…MicroTom / B. cinerea positive [ 101 ] miR482b Tomato cv. MicroTom / B. cinerea negative [ 102 ] miR1127-3p / WRKY75 Tomato ( Solanum lycopersicum cv. Ailsa Craig) / B. cinerea strain B05.10 positive (miRNA repression) [ 30 ] up-regulation of GhmiR395 / APS1/3, adenosine 5′-phosphosulfate; down-regulation of GhmiR165 / REV- transcription factors Cotton ( Gossypium hirsutum L.) / V. dahliae positive [ 103 ] miR398b / NBS-LRR gene and cleavage of the mRNAs of superoxide dismutase and Copper chaperone for superoxide dismutase Cotton ( G. hirsutum cv.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic weapons of plants against fungal pathogens

Mierziak,

Wojtasik

2024

BMC Plant Biol

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In the natural environment, plants face constant exposure to biotic stress caused by fungal attacks. The plant’s response to various biotic stresses relies heavily on its ability to rapidly adjust the transcriptome. External signals are transmitted to the nucleus, leading to activation of transcription factors that subsequently enhance the expression of specific defense-related genes. Epigenetic mechanisms, including histone modifications and DNA methylation, which are closely linked to chromatin states, regulate gene expression associated with defense against biotic stress. Additionally, chromatin remodelers and non-coding RNA play a significant role in plant defense against stressors. These molecular modifications enable plants to exhibit enhanced resistance and productivity under diverse environmental conditions. Epigenetic mechanisms also contribute to stress-induced environmental epigenetic memory and priming in plants, enabling them to recall past molecular experiences and utilize this stored information for adaptation to new conditions. In the arms race between fungi and plants, a significant aspect is the cross-kingdom RNAi mechanism, whereby sRNAs can traverse organismal boundaries. Fungi utilize sRNA as an effector molecule to silence plant resistance genes, while plants transport sRNA, primarily through extracellular vesicles, to pathogens in order to suppress virulence-related genes. In this review, we summarize contemporary knowledge on epigenetic mechanisms of plant defense against attack by pathogenic fungi. The role of epigenetic mechanisms during plant-fungus symbiotic interactions is also considered.

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Molecular mechanism of modulating miR482b level in tomato with botrytis cinerea infection

Cited by 12 publications

References 61 publications

Screening of differentially expressed microRNAs and target genes in two potato varieties under nitrogen stress

Screening of differentially expressed microRNAs and target genes in two potato varieties under nitrogen stress

MIR390 Is Involved in Regulating Anthracnose Resistance in Apple

Epigenetic weapons of plants against fungal pathogens

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