Molecular Mechanism of microRNA396 Mediating Pistil Development in Arabidopsis

Liang, Gang; He, Hua; Li, Yang; Wang, Fang; Yu, Diqiu

doi:10.1104/pp.113.225144

Cited by 158 publications

(175 citation statements)

References 38 publications

Supporting

Mentioning

169

Contrasting

Order By: Relevance

“…The grf5 single mutant is indistinguishable from the wild type with regard to floral organ development. grf5 35S:MIR396a also showed similar phenotypes (Liang et al, 2014). These results indicate that a substantial reduction in the level of GRF mRNAs as a whole leads to the lack of CMMs and ovary valves.…”

supporting

confidence: 53%

“…Given the tight coupling of the GRF-GIF duo in leaf growth, it is also conceivable that grf mutations, like gif, should cause similar aberrancies in floral development. It was reported that some strong overexpressors of MIR396a often produced singlecarpel gynoecia (Liang et al, 2014;Pajoro et al, 2014). However, those studies did not resolve anatomical and histological details of how the down-regulation of miR396-targeted GRFs led to the phenotypes.…”

mentioning

confidence: 99%

“…In Arabidopsis, miR396 is encoded by MIR396a and MIR396b and induces the cleavage of seven GRF mRNAs, with the exceptions of GRF5 and GRF6 transcripts that lack the target site (Jones-Rhoades et al, 2006;Liu et al, 2009;Rodriguez et al, 2010). Overexpression of MIR396 using the cauliflower mosaic virus 35S promoter (35S:MIR396a and 35S:MIR396b) caused remarkable reductions in the levels of target GRF mRNAs in leaves and floral organs (Liu et al, 2009;Rodriguez et al, 2010;Liang et al, 2014). The existence of miR396 not only revealed an additional regulatory layer in the control of GRF expression but also provided a genetic tool for overcoming the functional redundancy of GRFs.…”

mentioning

confidence: 99%

See 2 more Smart Citations

GROWTH-REGULATING FACTOR and GRF-INTERACTING FACTOR Specify Meristematic Cells of Gynoecia and Anthers

Lee

Jung

et al. 2017

Plant Physiol.

View full text Add to dashboard Cite

We investigated the biological roles of the Arabidopsis (Arabidopsis thaliana) GROWTH-REGULATING FACTOR (GRF) and GRF-INTERACTING FACTOR (GIF) transcriptional complex in the development of gynoecia and anthers. There are nine GRFs and three GIFs in Arabidopsis, and seven GRFs are posttranscriptionally silenced by microRNA396 (miR396). We found that overexpression of MIR396 in the gif1 gif2 double mutant background (gif1 gif2 35S:MIR396) resulted in neither ovary nor pollen. Histological and molecular marker-based analyses revealed that the mutant gynoecial primordia failed to develop carpel margin meristems and mature flowers lacked the ovary, consisting only of the stigma, style, and replum-like tissues. The mutant anther primordia were not able to form the pluripotent archesporial cells that produce pollen mother cells and microsporangia. Multiple combinations of GRF mutations also displayed the same phenotypes, indicating that the GRF-GIF duo is required for the formation of those meristematic and pluripotent cells. Most GRF proteins are localized and abundant in those cells. We also found that the weak gynoecial defects of pinoid-3 (pid-3) mutants were remarkably exacerbated by gif1 gif2 double mutations and 35S:MIR396, so that none of the gynoecia produced by gif1 gif2 pid-3 and 35S:MIR396 pid-3 developed ovaries at all. Moreover, gif1 gif2 double mutations and 35S:MIR396 also acted synergistically with 1-N-naphthylphthalamic acid in forming aberrant gynoecia. The results altogether suggest that the GRF-GIF duo regulates the meristematic and pluripotent competence of carpel margin meristems and the archesporial cell lineage and that this regulation is implemented in association with auxin action, ultimately conferring reproductive competence on Arabidopsis.

show abstract

supporting

confidence: 53%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

GROWTH-REGULATING FACTOR and GRF-INTERACTING FACTOR Specify Meristematic Cells of Gynoecia and Anthers

Lee

Jung

et al. 2017

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…7C; Liang et al, 2014). Two individual CrMIR396 plants (lines 8 and 27) exhibited 4.6-and 3.5-fold increased miR396 expression compared with Col-0 plants.…”

Section: Flowering Phenotypes Of Mir396 In Loss and Gain Of Functionmentioning

confidence: 99%

MicroRNA396-Targeted SHORT VEGETATIVE PHASE Is Required to Repress Flowering and Is Related to the Development of Abnormal Flower Symptoms by the Phyllody Symptoms1 Effector

Yang

Huang²,

Lin³

et al. 2015

Plant Physiol.

View full text Add to dashboard Cite

ORCID IDs: 0000-0002-9951-687X (C.-P.L.); 0000-0002-7295-5004 (S.-S.L.).Leafy flowers are the major symptoms of peanut witches' broom (PnWB) phytoplasma infection in Catharanthus roseus. The orthologs of the phyllody symptoms1 (PHYL1) effector of PnWB from other species of phytoplasma can trigger the proteasomal degradation of several MADS box transcription factors, resulting in leafy flower formation. In contrast, the flowering negative regulator gene SHORT VEGETATIVE PHASE (SVP) was up-regulated in PnWB-infected C. roseus plants, but most microRNA (miRNA) genes had repressed expression. Coincidentally, transgenic Arabidopsis (Arabidopsis thaliana) plants expressing the PHYL1 gene of PnWB (PHYL1 plants), which show leafy flower phenotypes, up-regulate SVP of Arabidopsis (AtSVP) but repress a putative regulatory miRNA of AtSVP, miR396. However, the mechanism by which PHYL1 regulates AtSVP and miR396 is unknown, and the evidence of miR396-mediated AtSVP degradation is lacking. Here, we show that miR396 triggers AtSVP messenger RNA (mRNA) decay using genetic approaches, a reporter assay, and high-throughput degradome profiles. Genetic evidence indicates that PHYL1 plants and atmir396a-1 mutants have higher AtSVP accumulation, whereas the transgenic plants overexpressing MIR396 display lower AtSVP expression. The reporter assay indicated that target-site mutation results in decreasing the miR396-mediated repression efficiency. Moreover, degradome profiles revealed that miR396 triggers AtSVP mRNA decay rather than miRNA-mediated cleavage, implying that AtSVP caused miR396-mediated translation inhibition. We hypothesize that PHYL1 directly or indirectly interferes with miR396-mediated AtSVP mRNA decay and synergizes with other effects (e.g. MADS box transcription factor degradation), resulting in abnormal flower formation. We anticipate our findings to be a starting point for studying the posttranscriptional regulation of PHYL1 effectors in symptom development.

show abstract

“…Many studies have reported that GRFs act as positive regulators of cell proliferation and thus determine the final size of lateral organs, such as cotyledons, leaves, and flowers (Horiguchi et al 2005;Kim and Kende 2004;Kim et al 2003;Liu et al 2009;Wang et al 2014). GRFs also play a role in development of female reproductive organs of A. thaliana (Liang et al 2014), and are expressed in rice embryos and maize ears, suggesting a possible role in regulation of seed growth and development (Ye et al 2004;Zhang et al 2008). A GRF gene of B. napus, BnGRF2, was proposed to be responsible for increases in seed weight, probably by regulating cell number and photosynthesis; these, in turn, might be also responsible for the increased production of seed oil (Liu et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Brassica rapa GROWTH-REGULATING FACTOR genes in Arabidopsis thaliana increases organ growth by enhancing cell proliferation

Hong

Kim

et al. 2017

J Plant Biotechnol

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors containing two conserved QLQ and WRC domains and play critical roles in regulating the growth and development of lateral organs, such as cotyledons, leaves, and flowers. To explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), the researchers isolated seven BrGRFs (BrGRF3-1, 3-2, 5, 7, 8-1, 8-2, and 9) and constructed BrGRF-overexpressing Arabidopsis thaliana plants (BrGRF-OX). BrGRF-OX plants developed larger cotyledons, leaves, and flowers as well as longer roots than the wild type. The increase in size of these organs were due to increases in cell number, but not due to cell size. BrGRF-OX plants also had larger siliques and seeds. Furthermore, BrGRF-OX seeds produced more oil than the wild type. RT-PCR analysis revealed that BrGRFs regulated expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, the data indicates that BrGRFs act as positive regulators of plant growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed oil production.

show abstract

Molecular Mechanism of microRNA396 Mediating Pistil Development in Arabidopsis

Cited by 158 publications

References 38 publications

GROWTH-REGULATING FACTOR and GRF-INTERACTING FACTOR Specify Meristematic Cells of Gynoecia and Anthers

GROWTH-REGULATING FACTOR and GRF-INTERACTING FACTOR Specify Meristematic Cells of Gynoecia and Anthers

MicroRNA396-Targeted SHORT VEGETATIVE PHASE Is Required to Repress Flowering and Is Related to the Development of Abnormal Flower Symptoms by the Phyllody Symptoms1 Effector

Overexpression of Brassica rapa GROWTH-REGULATING FACTOR genes in Arabidopsis thaliana increases organ growth by enhancing cell proliferation

Contact Info

Product

Resources

About