Molecular Mechanism of Magnetic Crystal Formation in Magnetotactic Bacteria

Arakaki, Atsushi; Tanaka, Masayoshi; Matsunaga, Tadashi

doi:10.1007/978-981-10-8069-2_2

Cited by 8 publications

(14 citation statements)

References 109 publications

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“…非核心基因中一些成员的功能和作用在现有研究中尚未得到揭示, 例如基因mamU及mamV. 目前研究者仅仅发现当这些基因被删除后, 磁小体合成没有受到影响或受到很轻微的影响 [7,8] . [22,37] .…”

Section: Msr-1中这3个基因的功能受损通常使磁铁矿晶体的unclassified

“…mad基因被认为可能包含在mamAB-like和mamAB-like*两个操纵子中 [22] , 而目前我们尚未获得man基因是否属于趋磁硝化螺旋菌的ma-mAB-like操纵子的明确信息 [37] . 这两类基因暂且归于 [7,8] .…”

Section: Msr-1中这3个基因的功能受损通常使磁铁矿晶体的unclassified

“…磁小体合成的操纵子和基因研究显示, 磁小体合成的整个过程是一个多步骤多基因协同合作的生物合成流程, 需要涉及40种以上的不同基因 [7] . 成) [8] . 这个描述与先前其他研究者对磁小体合成过程的拆分大致一致, 但它给淡水磁螺菌AMB-1中磁小体合成的每一个步骤都提供了较全面的基因汇总.…”

Section: 磁小体合成的模型unclassified

“…实际上, 最近十几年来多位学者例如美国的Komeili [9] 、加拿大的Goldhawk等人 [10] 、中国的Yan等人 [11] 、以色列的Barber-Zucker等人 [12] . 相对地, 另一些学者则十分关注生物矿化部分, 将这一部分细分成铁离子摄取及生物矿化起始、氧化还原电位控制、晶体大小和尺寸控制等几部分 [8,12,45] . 除了研究侧重点的差异外, 不同 , 而另一些基因例如mamA编码的蛋白则为磁小体膜形成搭建了一个"脚手架"以确保膜结构的改造顺利完成 [46,47] .…”

Section: 磁小体合成的模型unclassified

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The operons and genes related to magnetosome biogenesis in magnetotactic bacteria

Yang

2019

Chin. Sci. Bull.

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Articles you may be interested in DMTB: A comprehensive online resource of 16S rRNA genes, ecological metadata, oligonucleotides, and magnetic properties of magnetotactic bacteria Chinese Science Bulletin 56, 476 (2011); Distribution of magnetotactic bacteria in China and characterization of magnetosomes Chinese Science Bulletin 41, 944 (1996); Characteristics of magnetotactic bacteria in Duanjiapo loess section, Shaanxi Province and their environment significance Science in China Series D-Earth Sciences 39, 478 (1996); Paleo-environmental study on the growth of magnetotactic bacteria and precipitation of magnetosomes in Chinese loess-paleosol sequences Chinese Science Bulletin 45, 21 (2000); An initial study of the influences of oxygen conditions on wild-type magnetotactic bacteria in sediment

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Section: Msr-1中这3个基因的功能受损通常使磁铁矿晶体的unclassified

Section: 磁小体合成的模型unclassified

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The operons and genes related to magnetosome biogenesis in magnetotactic bacteria

Yang

2019

Chin. Sci. Bull.

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“…Using magnetotactism coupled to a system of oxygen detection (aerotactism), MTB then use their flagella to propel themselves toward the oxic-anoxic transition zone, where the oxygen concentration is optimal for their development and for magnetosome synthesis (Lefèvre and Bazylinski, 2013). Magnetosomes consist of magnetite minerals surrounded by a biological membrane (e.g., Faivre and Schüler, 2008;Le Fèvre et al, 2017;Arakaki et al, 2018). Using these bacteria, it has been possible to produce nanoparticles that fulfill most of the criteria mentioned above (Alphandéry, 2014).…”

Section: Introductionmentioning

confidence: 99%

A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media

Berny

Fèvre²,

Guyot

et al. 2020

Front. Bioeng. Biotechnol.

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We report the synthesis in large quantity of highly pure magnetosomes for medical applications. For that, magnetosomes are produced by MSR-1 Magnetospirillum gryphiswaldense magnetotactic bacteria using minimal growth media devoid of uncharacterized and toxic products prohibited by pharmaceutical regulation, i.e., yeast extract, heavy metals different from iron, and carcinogenic, mutagenic and reprotoxic agents. This method follows two steps, during which bacteria are first pre-amplified without producing magnetosomes and are then fed with an iron source to synthesize magnetosomes, yielding, after 50 h of growth, an equivalent OD 565 of ∼8 and 10 mg of magnetosomes in iron per liter of growth media. Compared with magnetosomes produced in non-minimal growth media, those particles have lower concentrations in metals other than iron. Very significant reduction or disappearance in magnetosome composition of zinc, manganese, barium, and aluminum are observed. This new synthesis method paves the way towards the production of magnetosomes for medical applications.

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Identifying magnetosome-associated genes in the extended CtrA regulon inMagnetospirillum magneticumAMB-1 using a combinational approach

Yang

Cao

2022

Briefings in Functional Genomics

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Magnetotactic bacteria (MTB) are worth studying because of magnetosome biomineralization. Magnetosome biogenesis in MTB is controlled by multiple genes known as magnetosome-associated genes. Recent advances in bioinformatics provide a unique opportunity for studying functions of magnetosome-associated genes and networks that they are involved in. Furthermore, various types of bioinformatics analyses can also help identify genes associated with magnetosome biogenesis. To predict novel magnetosome-associated genes in the extended CtrA regulon, we analyzed expression data of Magnetospirillum magneticum AMB-1 in the GSE35625 dataset in NCBI GEO. We identified 10 potential magnetosome-associated genes using a combinational approach of differential expression analysis, Gene ontology and Kyoto encyclopedia of genes and genomes pathway enrichment analysis, protein–protein interaction network analysis and weighted gene co-expression network analysis. Meanwhile, we also discovered and compared two co-expression modules that most known magnetosome-associated genes belong to. Our comparison indicated the importance of energy on regulating co-expression module structures for magnetosome biogenesis. At the last stage of our research, we predicted at least four real magnetosome-associated genes out of 10 potential genes, based on a comparison of evolutionary trees between known and potential magnetosome-associated genes. Because of the discovery of common subtrees that the stressed species are enriched in, we proposed a hypothesis that multiple types of environmental stress can trigger magnetosome evolution in different waters, and therefore its evolution can recur at different times in various locations on earth. Overall, our research provides useful information for identifying new MTB species and understanding magnetosome biogenesis.

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Molecular Mechanism of Magnetic Crystal Formation in Magnetotactic Bacteria

Cited by 8 publications

References 109 publications

The operons and genes related to magnetosome biogenesis in magnetotactic bacteria

The operons and genes related to magnetosome biogenesis in magnetotactic bacteria

A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media

Identifying magnetosome-associated genes in the extended CtrA regulon inMagnetospirillum magneticumAMB-1 using a combinational approach

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