Molecular Mechanism of Detection of Aflatoxins and Other Mycotoxins

Stark, Avishay‐Abraham

doi:10.1007/978-3-642-00725-5_2

Cited by 6 publications

(26 citation statements)

References 47 publications

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“…The competition ELISA approaches that of the LC-MS method [44]. However, ELISA has been noticed to be not fully reliable due to cross-reaction interferences, especially at concentrations lower than 0.05 µg/L [44]. Magliulo et al [45] reported a more specific chemiluminescent assay reaching 0.001µg/L.…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 98%

“…In the control well (absence of mycotoxin in the sample), the mycotoxin-enzyme conjugate can saturate the bound antibody, and addition of a chromogenic substrate results in the development of colour. In the test well, free mycotoxin molecules in the extract compete with the conjugate on the bound antibody [44]. The higher the concentration of mycotoxin, the less the conjugate can react with the bound antibody, leading to fainter colour development [44].…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

“…In the test well, free mycotoxin molecules in the extract compete with the conjugate on the bound antibody [44]. The higher the concentration of mycotoxin, the less the conjugate can react with the bound antibody, leading to fainter colour development [44]. The competition ELISA approaches that of the LC-MS method [44].…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

“…The higher the concentration of mycotoxin, the less the conjugate can react with the bound antibody, leading to fainter colour development [44]. The competition ELISA approaches that of the LC-MS method [44]. However, ELISA has been noticed to be not fully reliable due to cross-reaction interferences, especially at concentrations lower than 0.05 µg/L [44].…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

“…Typical lateral flow immunochromatography (LFIA) strip is composed of a loading pad where a sample of the extract is applied; a zone containing coloured particles (e.g., latex, gold) coated with a mouse monoclonal antimycotoxin antibody; a zone of nitrocellulose membrane that allows the migration of the particles together with the mycotoxin sample; a test line that contains immobilized mycotoxin; a positive control line that contains a secondary anti-mouse antibody, and an absorbent pad [44]. In principle the LFIA is that AFM1 extract is applied and migrates along the strip and once the conjugate zone is reached, the mycotoxin binds the anti mycotoxinparticle complex.…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

See 4 more Smart Citations

A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review

Mwanza¹,

Ngoma²,

Nyirenda³

et al. 2013

Mycotoxin and Food Safety in Developing Countries

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Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 98%

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

See 3 more Smart Citations

A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review

Mwanza¹,

Ngoma²,

Nyirenda³

et al. 2013

Mycotoxin and Food Safety in Developing Countries

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Assessment of aflatoxin M1 and B1 in some dairy products with referring to the analytical performances of enzyme-linked immunosorbent assay in comparison to high-performance liquid chromatography

Esam

Hafez

Khafaga³

et al. 2022

Vet World

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Background and Aim: Aflatoxin M1 (AFM1) is a major fungal metabolite found in milk coming from aflatoxin B1 (AFB1) contaminated rations and is subsequently present in milk-based products demonstrating a serious public health hazard. This study aimed to investigate the levels of AFM1 and AFB1 in milk and some dairy products consumed widely by infants and children. Materials and Methods: This study investigated the incidence of AFM1 in 105 samples of processed cheese, Ras cheese, and raw milk (35 of each) retailed in the Egyptian markets. The degree of sensitivity and accuracy was evaluated using the enzyme-linked immunosorbent assay (ELISA) method followed by the estimation of the positive samples using the high-performance liquid chromatography (HPLC) with fluorescence detection. Mold count was determined in the examined samples by investigating AFB1 content using HPLC. Results: AFM1 was found in all investigated Ras cheese, raw milk, and 82.86% of the processed cheese samples with mean values of 51.05±6.19, 40.27±3.996, and 10.77±1.39 ng/kg, respectively. Moreover, there was statistically no significant difference between AFM1 levels in the core and crust parts of the tested Ras cheese. AFM1 contaminated Ras cheese and raw milk samples were 48.57% and 25.71%, which exceeded the European and Egyptian tolerance levels. Results showed an acceptable correlation between ELISA and HPLC methods with no significant difference (p>0.05). Alternatively, none of the examined samples proved to be contaminated with AFB1 despite the presence of mold with mean counts of 3.79±3.29, 4.39±4.34, and 4.84±4.29 log CFU/g in the examined processed cheese, Ras cheese, and raw milk samples, respectively. Conclusion: Therefore, it is urgent to regularly inspect the contamination of animal feeds with AFB1 and apply special measures and novel techniques to protect the feed and food from public health hazards.

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Gıda, Balık ve Mikotoksinler

Sanchez

Torres

Espinosa-Chaurand

2020

Acta Aquatica Turcica

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Due to their incidence and negative effects on public health, foodborne diseases are considered an issue of international relevance. Fish is considered a nutritious food and highly susceptible to contamination by physical, chemical, and biological agents responsible for diseases in animals and humans. Mycotoxins are biological contaminants of food with various harmful effects on human and animal health derived from their consumption, concentration, and exposure time. The presence of mycotoxins has been reported in different foods, mainly cereals, which are intended for human and animal consumption, such as feed processing. The presence of mycotoxins in fish is generally by way of feeding by supplying contaminated feed in aquaculture production activities, and the transfer can be generated in the food chain and affect human health. The objective of this bibliographic review is to provide relevant general information about mycotoxins in foods including fish, harmful effects on fish, especially tilapia, prevention, control, and regulation of these contaminants in foods intended for human and animal consumption, its negative impact on animal production and health, as well as human health through its transfer in the food chain.

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Molecular Mechanism of Detection of Aflatoxins and Other Mycotoxins

Cited by 6 publications

References 47 publications

A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review

A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review

Assessment of aflatoxin M1 and B1 in some dairy products with referring to the analytical performances of enzyme-linked immunosorbent assay in comparison to high-performance liquid chromatography

Gıda, Balık ve Mikotoksinler

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