Molecular mechanism of cell proliferation in rodent uterus during the estrous cycle

Baranda-Ávila, Noemi; Mendoza-Rodríguez, C. Adriana; Morimoto, Sumiko; Langley, Elizabeth; Cerbón, Marco

doi:10.1016/j.jsbmb.2009.01.008

Cited by 11 publications

(11 citation statements)

References 48 publications

(65 reference statements)

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“…Given that serum LH levels are much lower in SD than in LD female hamsters (Darrow et al 1980, Dodge & Badura 2002, it is possible that FSH-dependent estrogen production by granulosa cells is substantially reduced due to a decrease in LH-induced androgen precursor production by theca cells. The observed lack of effect of rhFSH on uterine mass and vaginal patency, which are largely estrogen-dependent traits (Odum et al 1997, van Meeuwen et al 2007, Suzuki et al 2007, Baranda-Avila et al 2009), supports these conclusions. Although LH levels do not immediately increase after transfer to LD (Simpson et al 1982, Meredith et al 1998, LH secretion may be necessary for the complete expression of the LD phenotype.…”

Section: Long-term Rhfsh Supplementation In Sdsmentioning

confidence: 57%

Photoperiod-gonadotropin mismatches induced by treatment with acyline or FSH in Siberian hamsters: impacts on ovarian structure and function

et al. 2012

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Many seasonal breeders time their reproductive efforts to specific times of the year to ensure adequate resources for the production and care of young. For long-day (LD) breeders, females born before the summer solstice (LDs) reach sexual maturity quickly and often breed that same year, whereas females born after the summer solstice (short days (SDs)) may delay reproductive development to the following spring when environmental conditions are favorable for reproduction. In Siberian hamsters, development in SD is associated with structural and functional differences in the ovary compared with females held in LD, including a greater number of primordial follicles and an abundance of hypertrophied granulosa cells (HGCs), which are immunoreactive for anti-Mü llerian hormone. The goal of this study was to determine whether SD-induced gonadotropin suppression is responsible for these phenotypic differences. Gonadotropin levels were suppressed in LD hamsters using the GNRH antagonist acyline. Conversely, to determine whether the SD ovarian phenotype is completely reversed by gonadotropin stimulation, recombinant human FSH (rhFSH) was administered. Our treatments were successful in mimicking FSH concentrations of the opposite photoperiod, but they did not produce a comparable change in the ovarian phenotype. Most notable was the lack of HGCs in the ovaries of acyline-treated LD females. Similarly, HGCs were maintained in the ovaries of SD females treated with rhFSH. Our data suggest that gonadotropins alone do not account for the SD ovarian phenotype. Future studies will determine whether SD-induced changes in other factors underlie these phenotypic changes.

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Section: Long-term Rhfsh Supplementation In Sdsmentioning

confidence: 57%

Photoperiod-gonadotropin mismatches induced by treatment with acyline or FSH in Siberian hamsters: impacts on ovarian structure and function

et al. 2012

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“…In utero exposure to MEHP did not significantly affect maternal body weight or pup growth at any of the doses examined (Table 1); however, we observed changes in the estrous cycle of exposed females at all doses examined (Table 2). We postulate that these changes may be due to alterations in normal hormonal signaling which extend to the uterine histology, as measured by vaginal smear cytology [32]. Altered estrous cyclicity has been reported in adult female rats following acute exposure to extremely high doses of MEHP [33].…”

Section: Discussionmentioning

confidence: 99%

Reproductive effects in F1 adult females exposed in utero to moderate to high doses of mono-2-ethylhexylphthalate (MEHP)

Moyer

Hixon

2012

Reproductive Toxicology

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Phthalates are widely used as plasticizers in everyday products. Yet, studies on the effects of phthalates on female reproductive health are limited. In this study, pregnant C57/Bl6 mice were exposed via oral gavage to corn oil, 100, 500, or 1000mg/kg MEHP from gestational days 17–19. Reproductive lifespan was decreased by one month in the highest F1 exposure group (9.8±0.4 versus 11.1±0.6 months in control F1 females). F1 females exhibited delayed estrous onset at the two higher exposures and prolonged estrus was observed in all MEHP-exposed females. Serum FSH and estradiol were significantly elevated at the highest exposure and altered mRNA expression was found for the steroidogenic genes LHCGR, aromatase, and StAR. At one year of age, mammary gland hyperplasia was observed in high dose MEHP-exposed females. In summary, late gestational exposure to MEHP leads to multiple latent reproductive effects throughout murine life resulting in premature ovarian senescence and mammary hyperplasia.

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“…EP2 regulates epithelial differentiation and implantation [3,12,23]. E2 is known to induce proliferation and changes in epithelium in preparation for implantation [24]. Thus, regulation of EP2 by E2 in epithelium may be important for implantation.…”

Section: Discussionmentioning

confidence: 99%

Prostaglandin receptors EP and FP are regulated by estradiol and progesterone in the uterus of ovariectomized rats

Selvanesan

Büttner

Masironi

et al. 2012

Reprod Biol Endocrinol

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BackgroundProstaglandins are important for female reproduction. Prostaglandin-E2 acts via four different receptor subtypes, EP1, EP2, EP3 and EP4 whereas prostaglandin-F2alpha acts through FP. The functions of prostaglandins depend on the expression of their receptors in different uterine cell types. Our aim was to investigate the expression of EPs and FP in rat uterus and to identify the regulation by estradiol, progesterone and estrogen receptor (ER) selective agonists.MethodsWe performed four different rat experiments involving treatments with estradiol, progesterone and ER agonists. Real-time PCR and immunohistochemistry were employed to evaluate receptor expression.ResultsOur results showed that all mRNAs and proteins of EPs and FP are expressed in the rat uterus. The expression pattern and intensity of immunostaining vary between different cell types and treatments. The mRNA expression of all EPs and FP are downregulated by estradiol and the ERalpha specific agonist PPT, whereas the ERbeta specific agonist DPN downregulates only EP2 and EP4. The protein expression however, showed an increase in EP2 and EP3 after estradiol treatment. When treated with estradiol and progesterone in combination, the expressions of EP1 and EP3 are upregulated.ConclusionsRegulation of EPs and FP expression by estradiol appears to be mainly modulated via ERalpha for EP1, EP3 and FP, while EP2 and EP4 also are affected by the ERbeta selective ligand. Our immunohistochemical data shows a cell specific regulation of prostaglandin receptors under the influence of ovarian steroids, where EP2 is estrogen regulated in all uterine tissues examined. EP1 and EP3 are upregulated by the combination of estradiol and progesterone. Thus, our observations indicate that estradiol and progesterone regulate the mRNA and protein expression of EPs and FP in a receptor and tissue specific way.

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Molecular mechanism of cell proliferation in rodent uterus during the estrous cycle

Cited by 11 publications

References 48 publications

Photoperiod-gonadotropin mismatches induced by treatment with acyline or FSH in Siberian hamsters: impacts on ovarian structure and function

Photoperiod-gonadotropin mismatches induced by treatment with acyline or FSH in Siberian hamsters: impacts on ovarian structure and function

Reproductive effects in F1 adult females exposed in utero to moderate to high doses of mono-2-ethylhexylphthalate (MEHP)

Prostaglandin receptors EP and FP are regulated by estradiol and progesterone in the uterus of ovariectomized rats

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