Molecular mechanism involved in epithelial to mesenchymal transition

Jayachandran, Jayashree; Srinivasan, Harini; Mani, Krishna Priya

doi:10.1016/j.abb.2021.108984

Cited by 45 publications

(42 citation statements)

References 125 publications

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“…In this process, epithelial cells lose their cell-to-cell adhesion capacity and gain mesenchymal characteristics. Via EMT, the cells develop increased migratory capabilities and gain a higher invasive and metastatic potential [ [345] , [346] , [347] , [348] ]. EMT is also closely associated with ‘tumor budding’, a process whereby isolated single cancer cells or small clusters of cancer cells appear at the invasive tumor front [ 349 , 350 ].…”

Section: Role Of Cbs In Cancer: An Integrated Conceptmentioning

confidence: 99%

Emerging roles of cystathionine β-synthase in various forms of cancer

Ascenção¹,

Szabó²

2022

Redox Biology

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Section: Role Of Cbs In Cancer: An Integrated Conceptmentioning

confidence: 99%

Emerging roles of cystathionine β-synthase in various forms of cancer

Ascenção¹,

Szabó²

2022

Redox Biology

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“…E-cadherin is a typical epithelial marker and it is regarded as the master regulator of cell adhesion and repressed by Snail family. On the contrary, Vimentin is a canonical mesenchymal marker and it is considered as the active player of cell migration and activated by Twist family ( Jayachandran, Srinivasan & Mani, 2021 ). In our study, knockdown of SPRY4-IT1 decreased the protein expression levels of Snail, Twist1 and Vimentin, and increased the protein expression level of E-cadherin, which suggested that knockdown of SPRY4-IT1 inhibited NPC cell metastasis might via the regulation of EMT pathway.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA SPRY4-IT1 promotes proliferation and metastasis in nasopharyngeal carcinoma cell

Liao

Wang

et al. 2022

PeerJ

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Background Long non-coding RNA SPRY4 intronic transcript 1 (Lnc RNA SPRY4-IT1) was aberrant-expressed in various kinds of cancer. Increasing evidence demonstrated that lnc RNAs involved in tumorigenesis and metastasis. In this study, we aimed to explore the biological role of SPRY4-IT1 on the phenotype of nasopharyngeal carcinoma (NPC) in vitro and in vivo. Methods The expression level of SPRY4-IT1 in NPC cell lines were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8 (CCK-8) and colony formation assay were used to detect cell proliferation. Wound-healing assay, transwell assay and animal experiment were performed to evaluate the ability of cell migration and metastasis. Cell cycle distribution and apoptosis were determined by flow cytometry. Western blotting and immunofluorescence were employed to identify protein expression. Results SPRY4-IT1 was significantly up-regulated in several NPC cell lines (6-10B, CNE-2, and HONE-1) compared with human immortalized nasopharyngeal epithelial cell (NP69). Silencing of SPRY4-IT1 inhibited proliferation, migration, and metastasis, and induced significant G2/M phase arrest and apoptosis. Western blotting showed that the expression levels of cell cycle-related proteins (cyclin B1, cdc2 and p-cdc2) were down-regulated and apoptosis-associated proteins (PARP, cleaved PARP and cleaved caspase-3) were up-regulated after knockdown of SPRY4-IT1. The expression level of E-cadherin was increased and the expression of Vimentin, Snail and Twist1 were decreased after the SPRY4-IT1 knockdown. Conclusion lncRNA SPRY4-IT1 played a significant role in NPC proliferation, migration and metastasis, suggesting that SPRY4-IT1 might be a potential therapeutic target for the treatment of NPC.

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“…Epithelial-mesenchymal cell crosstalk is thought to contribute to reprogramming epithelial cell identity with a foetal-like phenotype during intestinal regeneration [ 31 ]. The capacity of epithelial cells to undergo epithelial-mesenchymal transition (EMT) particularly as a result of the activation of foetal-like programmes also demonstrate cellular and tissue plasticity [ 32 ]. Crypts overlying granulomas caused by mucosal invasion of Heligmosomoides polygyrus lost Lgr5, Olmf4 and other stem cell-associated expressions, but crypts became hyper-proliferative and less differentiated as a result of an IFNγ-dependent transcriptional programme that was enriched in genes normally expressed in the foetal intestinal epithelium such as Sca-1 (Ly6a) [ 33 ].…”

Section: Key Intestinal Stem Cell Niche Componentsmentioning

confidence: 99%

Identifying key regulators of the intestinal stem cell niche

Duckworth

2021

Biochemical Society Transactions

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The intestinal tract is lined by a single layer of epithelium that is one of the fastest regenerating tissues in the body and which therefore requires a very active and exquisitely controlled stem cell population. Rapid renewal of the epithelium is necessary to provide a continuous physical barrier from the intestinal luminal microenvironment that contains abundant microorganisms, whilst also ensuring an efficient surface for the absorption of dietary components. Specialised epithelial cell populations are important for the maintenance of intestinal homeostasis and are derived from adult intestinal stem cells (ISCs). Actively cycling ISCs divide by a neutral drift mechanism yielding either ISCs or transit-amplifying epithelial cells, the latter of which differentiate to become either absorptive lineages or to produce secretory factors that contribute further to intestinal barrier maintenance or signal to other cellular compartments. The mechanisms controlling ISC abundance, longevity and activity are regulated by several different cell populations and signalling pathways in the intestinal lamina propria which together form the ISC niche. However, the complexity of the ISC niche and communication mechanisms between its different components are only now starting to be unravelled with the assistance of intestinal organoid/enteroid/colonoid and single-cell imaging and sequencing technologies. This review explores the interaction between well-established and emerging ISC niche components, their impact on the intestinal epithelium in health and in the context of intestinal injury and highlights future directions and implications for this rapidly developing field.

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Molecular mechanism involved in epithelial to mesenchymal transition

Cited by 45 publications

References 125 publications

Emerging roles of cystathionine β-synthase in various forms of cancer

Emerging roles of cystathionine β-synthase in various forms of cancer

Long non-coding RNA SPRY4-IT1 promotes proliferation and metastasis in nasopharyngeal carcinoma cell

Identifying key regulators of the intestinal stem cell niche

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