Molecular Markers Mapped around the High Shoot Regeneration Capacity Gene Rg-2 in Lycopersicon chilense.

Sato, Hiroki; Takashina, Tadashi; Escalante, Alice; Egashira, Hiroaki; Imanishi, Shigeru

doi:10.1270/jsbbs.50.251

Cited by 10 publications

(27 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Satoh et al [22] mapped a putative allele ( Rg-2 ) from S. chilense on this chromosome. The acid invertase gene, reported as a marker linked to Rg-2 , was chosen for mapping Rg-2 in our population derived from S. pennellii .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Localization of QTLs for in vitroplant regeneration in tomato

et al. 2011

View full text Add to dashboard Cite

BackgroundLow regeneration ability limits biotechnological breeding approaches. The influence of genotype in the regeneration response is high in both tomato and other important crops. Despite the various studies that have been carried out on regeneration genetics, little is known about the key genes involved in this process. The aim of this study was to localize the genetic factors affecting regeneration in tomato.ResultsWe developed two mapping populations (F2 and BC1) derived from a previously selected tomato cultivar (cv. Anl27) with low regeneration ability and a high regeneration accession of the wild species Solanum pennellii (PE-47). The phenotypic assay indicated dominance for bud induction and additive effects for both the percentage of explants with shoots and the number of regenerated shoots per explant. Two linkage maps were developed and six QTLs were identified on five chromosomes (1, 3, 4, 7 and 8) in the BC1 population by means of the Interval Mapping and restricted Multiple QTL Mapping methods. These QTLs came from S. pennellii, with the exception of the minor QTL located on chromosome 8, which was provided by cv. Anl27. The main QTLs correspond to those detected on chromosomes 1 and 7. In the F2 population, a QTL on chromosome 7 was identified on a similar region as that detected in the BC1 population. Marker segregation distortion was observed in this population in those areas where the QTLs of BC1 were detected. Furthermore, we located two tomato candidate genes using a marker linked to the high regeneration gene: Rg-2 (a putative allele of Rg-1) and LESK1, which encodes a serine/threonine kinase and was proposed as a marker for regeneration competence. As a result, we located a putative allele of Rg-2 in the QTL detected on chromosome 3 that we named Rg-3. LESK1, which is also situated on chromosome 3, is outside Rg-3. In a preliminary exploration of the detected QTL peaks, we found several genes that may be related to regeneration.ConclusionsIn this study we have identified new QTLs related to the complex process of regeneration from tissue culture. We have also located two candidate genes, discovering a putative allele of the high regeneration gene Rg-1 in the QTL on chromosome 3. The identified QTLs could represent a significant step toward the understanding of this process and the identification of other related candidate genes. It will also most likely facilitate the development of molecular markers for use in gene isolation.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In addition, a putative allele of Rg-1 from S . chilense (Dunal) Reiche ( Rg-2 ) was reported by Takashina et al [9] and Satoh et al [22]. Both alleles may act in combination with other alleles of either tomato or the wild relatives S. peruvianum or S. chilense [22,25].…”

Section: Introductionmentioning

confidence: 99%

Localization of QTLs for in vitroplant regeneration in tomato

et al. 2011

View full text Add to dashboard Cite

show abstract

“…In this regard, reported a regeneration ability in somatic tissues from the wild species C. praetermissum higher than cultivated species of Capsicum and, also, similar results have been reported in wild relatives of the tomato (e.g. S. peruvianum, S. chilense) Satoh et al, 2000). Thus, it seems that domestication may have decreased the in vitro culture ability of cultivated species and, therefore, this might explain the performance of C. pubescens, which has been submitted to the least intense domestication process and breeding efforts among the cultivated Capsicums (DeWitt and .…”

Section: Effect Of the Genotype And The Stage Of Development (G S Ansupporting

confidence: 79%

Técnicas y estrategias de mejora para facilitar la hibridación interespecífica y el acortamiento del ciclo generacional en el género Capsicum

Poblete¹,

Andrés²

View full text Add to dashboard Cite

Common pepper (Capsicum annuum L.) is among the most important vegetables in the world and huge breeding efforts are made every year to develop new improved materials of this species. In this regard, in vitro culture of immature embryos may help breeders to accelerate breeding cycles, to overcome interspecific barriers, among other applications. In this work, we have optimized a protocol for in vitro culture of immature embryos of C. annuum. Different levels (0.01 and 0.2 mg L-1) of indole-3-acetic acid (IAA) and zeatin have been tested to improve the efficiency (germination rates) of this technique in C. annuum embryos at the four main immature stages (i.e. globular, heart, torpedo, and early cotyledonary) from four different varietal types of this species (California Wonder, Piquillo, Guindilla, and Bola). The effect of 5-days initial incubation in dark was also tested on the most efficient hormone formulation. On average, relatively low levels of both IAA and zeatin (0.01 mg L-1 each) (M 1) provided the highest germination rates (33%), particularly in the advanced stages (torpedo and cotyledonary). To a lower extent, the lack of these growth regulators or high IAA (0.2 mg L-1)/low zeatin (0.01 mg L-1) combination also showed a positive response of embryos (24%). On the contrary, high zeatin levels (0.2 mg L-1) provoked very low germination rates or callus development (efficiency 0-7% at any stage and genotype). Finally, M 1 plus 5-days of initial dark incubation (M 1-D) improved the efficiency rates at any embryo stage, particularly in the earliest (globular) embryos which increased from 3% to >20%.

show abstract

“…peruvianum (Koornneef et al, 1993), S. pennellii (Arikita et al, 2012), S. pimpinellifolium (De Faria et al, 2002) and S. chilense (Satoh et al, 2000) are also highly regenerant, while most cultivated varieties of S. lycopersicum are largely recalcitrant. This suggests that high regeneration and associated traits are shared by tomato wild genotypes although they may display allelic variation in the same locus.…”

Section: Appendix Heritability Of the High Regeneration Capacity In Tmentioning

confidence: 99%

“…Furthermore, as shown by Szinay et al (2012), synteny has been well preserved on chromosome 3, thus enabling us to compare the Rg1 locus across domesticated and wild species. (Satoh et al, 2000) identified an Rg1 allele in S. chilense cv. PI128644 located on chromosome 3 by analyzing linkage between high shoot regeneration from root explants with markers based on random amplified polymorphic DNA (RAPD) and RFLP.…”

Section: Appendix Heritability Of the High Regeneration Capacity In Tmentioning

confidence: 99%

The Quest for a Key Gene Controlling Acquisition of Cell Competence During In Vitro Organogenesis in Tomato

Abeyratne¹

View full text Add to dashboard Cite

Plants show a remarkable capacity for vegetative propagation through de novo organogenesis. Unlike their wild relatives, most commercially important crop species lack this ability, which is largely attributed to their inability to acquire regeneration competence by dedifferentiation of somatic cells. In vitro micropropagation relies heavily on overcoming such regeneration recalcitrance for its success in biotechnology and clonal propagation in agriculture. Here, we investigate a key locus (Regeneration1) responsible for acquisition of competence that confers high rates of de novo shoot and root organogenesis in tomato. In vitro shoot regeneration assay was performed in several Solanum pennellii introgression lines into S. lycopersicum cv. Micro-Tom (MT) to help identify 29 candidate RG1 genes from a genomic region that originally occupied 6.16 Mbps with 293 genes. We used recalcitrant genotype MT as well as the highly regenerant S. pennellii and its introgression line MT-Rg3C in a subsequent in vitro shoot regeneration assay to characterize the expression of candidate genes using RT-qPCR. Two candidate genes were upregulated in both S. pennellii and MT-Rg3C in comparison to MT. Since the dominant recalcitrance trait may be conferred by mutations leading to lack of function in MT, sequence variations of the 29 candidates were compared between the S. pennellii and MT genomes. Ten genes were identified with mutations that may cause loss of function. Furthermore, 11 novel proteins with unknown functions were identified with polymorphisms for further investigation. Here we propose at least 10 candidate genes for functional characterization in other highly regenerant genotypes to ultimately end the quest for a key gene controlling organogenesis in tomato.

show abstract

Molecular Markers Mapped around the High Shoot Regeneration Capacity Gene Rg-2 in Lycopersicon chilense.

Cited by 10 publications

References 22 publications

Localization of QTLs for in vitroplant regeneration in tomato

Localization of QTLs for in vitroplant regeneration in tomato

Técnicas y estrategias de mejora para facilitar la hibridación interespecífica y el acortamiento del ciclo generacional en el género Capsicum

The Quest for a Key Gene Controlling Acquisition of Cell Competence During In Vitro Organogenesis in Tomato

Contact Info

Product

Resources

About