Molecular markers for the investigation of Mycobacterium gordonae epidemics

Vogiatzakis, E.; Stefanou, S.; Skroubelou, A.; Anagnostou, S.; Marinis, E.; Matsiota-Bernard, P.

doi:10.1016/s0195-6701(98)90277-8

Cited by 10 publications

(10 citation statements)

References 9 publications

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“…A combination of several primer sets yielded RAPD profiles that were at least comparably polymorphic with those of PFGE (180). RAPD analysis was also applied for genotyping of M. abscessus and M. chelonae, which often lyse spontaneously during gel electrophoresis and cannot be assessed by PFGE (165,174), as well as for M. phocaicum (181), M. gordonae (182), M. szulgai (183), and M. malmoense (184). Abed et al performed RAPD analysis on M. tuberculosis strains by using a 16-23S rRNA gene ITS as a PCR template, which gave the method higher discriminatory power (185).…”

Section: Methods Based On Nonrepetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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Section: Methods Based On Nonrepetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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“…Variations in RAPD patterns are chiefly attributed to variations in the priming efficiency during early rounds of amplification, and these in turn depend on template concentration and purity, primer/template ratio, or the ramp times of the cyclers used. Nonetheless, the RAPD typing has been used to differentiate among isolates of M. tuberculosis complex isolates [161–164] as well as among isolates of numerous NTM species, including M. abscessus [165], M. phocaicum [166], M. gordonae [167], M. szulgai [168], and M. malmoense [169]. …”

Section: Random Amplified Polymorphic Dna (Rapd) Analysismentioning

confidence: 99%

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

Jagielski

Ingen

Rastogi

et al. 2014

BioMed Research International

121

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In the epidemiology of tuberculosis (TB) and nontuberculous mycobacterial (NTM) diseases, as in all infectious diseases, the key issue is to define the source of infection and to disclose its routes of transmission and dissemination in the environment. For this to be accomplished, the ability of discerning and tracking individual Mycobacterium strains is of critical importance. Molecular typing methods have greatly improved our understanding of the biology of mycobacteria and provide powerful tools to combat the diseases caused by these pathogens. The utility of various typing methods depends on the Mycobacterium species under investigation as well as on the research question. For tuberculosis, different methods have different roles in phylogenetic analyses and person-to-person transmission studies. In NTM diseases, most investigations involve the search for environmental sources or phylogenetic relationships. Here, too, the type of setting determines which methodology is most suitable. Within this review, we summarize currently available molecular methods for strain typing of M. tuberculosis and some NTM species, most commonly associated with human disease. For the various methods, technical practicalities as well as discriminatory power and accomplishments are reviewed.

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“…The patient was then re-hospitalized and underwent surgical drainage of the abscesses and a second culture from the smear. Contemporaneously, we obtained biochemical identification of the mycobacterium strain isolated from the abscesses as Mycobacterium gordonae (whose isolation was later confirmed in the second culture), diagnosis confirmed by microarray assay (LCD-Array Myco) DNA based identification of Mycobacterium tuberculosis complex (MTUB) and other Mycobacteria (MOT) [13,14]. In accordance with the results of the drug sensitivity test (Table 1), oral ciprofloxacin (1 g daily) was given.…”

Section: Case Presentationmentioning

confidence: 99%

Cutaneous manifestations of Mycobacterium gordonae infection described for the first time in Italy: a case report

Foti

Sforza²,

Rizzo

et al. 2009

Cases J

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IntroductionMycobacterium gordonae is one of the least pathogenic of the mycobacteria. This pathogen may produce caseating or non-caseating granulomas, and skin lesions showing acute or chronic inflammation with scattered histiocytes and giant cells have been seen. The mortality rate is less than 0.1%. Mycobacterium gordonae may be a marker of severe immunosuppression in patients infected with human immunodeficiency virus.Case presentationWe report a case of Mycobacterium gordonae infection in an 86-year-old woman and discuss the problems inherent to the identification and treatment of this emerging pathogen. Mycobacterium gordonae strain we isolated was resistant to trimethoprim-sulfamethoxazole but sensitive to ciprofloxacin, and long term administration (six months) induced complete healing of the cutaneous abscesses.ConclusionAdvanced laboratory diagnostic techniques have improved the isolation and identification of nontuberculous mycobacteria. The diagnosis requires a high index of clinical suspicion, as detection by conventional methods is difficult. To our knowledge, this patient is the first documented case of cutaneous infection from this pathogen in Italy.

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Molecular markers for the investigation of Mycobacterium gordonae epidemics

Cited by 10 publications

References 9 publications

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

Cutaneous manifestations of Mycobacterium gordonae infection described for the first time in Italy: a case report

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