Molecular markers for systematic identification and population genetics of the invasive Ponto‐Caspian freshwater gammarid <i>Dikerogammarus villosus</i> (Crustacea, Amphipoda)

Wattier, Rémi A.; Beguet, Jérèmie; Gaillard, María Emilia; Müller, Jakob; Bollache, Loı̈c; Perrot‐Minnot, Marie‐Jeanne

doi:10.1111/j.1471-8286.2006.01283.x

Cited by 9 publications

(14 citation statements)

References 9 publications

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“…All D. villosus individuals were genotyped using species-specific microsatellite markers (Wattier et al 2006) with forward primers fluorescently-labelled as follows: DikF(6FAM), DikQ(VIC), DikS(PET). All primers were combined in a single multiplex PCR reaction.…”

Section: Population Geneticsmentioning

confidence: 99%

Enemy release and genetic founder effects in invasive killer shrimp populations of Great Britain

et al. 2014

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Arundell, K., Dunn, A., Alexander, J. L., Shearman, R., Archer, N., Ironside, J. (2015). Enemy release and genetic founder effects in invasive killer shrimp populations of Great Britain. Biological Invasions, 17 (5), 1439-1451. Document embargo until 04/11/2015The predatory ?killer shrimp? Dikerogammarus villosus invaded Britain from mainland Europe in 2010. Originating in the Ponto-Caspian region, this invader has caused significant degradation of European freshwater ecosystems by predating and competitively excluding native invertebrate species. In contrast to continental Europe, in which invasions occurred through the migration of large numbers of individuals along rivers and canals, the invasion of Great Britain must have involved long distance dispersal across the sea. This makes the loss of genetic diversity and of debilitating parasites more likely. Analysis of nuclear microsatellite loci and mitochondrial DNA sequences of D. villosus samples from the four known populations in Britain reveal loss of rare alleles, in comparison to reference populations from the west coast of continental Europe. Screening of the British D. villosus populations by PCR detected no microsporidian parasites, in contrast with continental populations of D. villosus and native amphipod populations, most of which are infected with microsporidia. These findings suggest that the initial colonisation of Great Britain and subsequent long distance dispersal within Britain were associated with genetic founder effects and enemy release due to loss of parasites. Such effects are also likely to occur during future long-distance dispersal events of D. villosus to Ireland or North America.authorsversionPeer reviewe

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Section: Population Geneticsmentioning

confidence: 99%

Enemy release and genetic founder effects in invasive killer shrimp populations of Great Britain

et al. 2014

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“…In addition, one sample collected in 1997 (date of appearance of D. villosus in France; n 030) was available from the SJL site. We were therefore able to compare the prevalence of microsporidia on this site in 1997, 2002 and 2005. DNA was extracted from all samples according to Wattier et al (2006).…”

Section: Population Samplingmentioning

confidence: 99%

No genetic bottleneck or associated microparasite loss in invasive populations of a freshwater amphipod

Wattier¹,

Haine²,

Beguet³

et al. 2007

Oikos

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Understanding what factors determine the success of an invasive species in its adopted range is crucial from an evolutionary ecology point of view, because it can provide insights into which biological characteristics are required for survival in varied environmental conditions. Successful establishment may depend on both maintaining genetic diversity, which will allow the species to evolve and/or adapt to new environments, and the presence or absence of natural enemies such as parasites. We tested these two hypotheses by studying populations of the amphipod crustacean Dikerogammarus villosus . This Ponto-Caspian invader has rapidly and successfully invaded western Europe and threatens macroinvertebrate biodiversity in its adopted ranges. It is a unique system to study since both its colonisation history and its geographic origins are well-known. Using samples from the whole geographic range of the invasion route, and using four molecular markers, we found no evidence for genetic bottlenecks during the invasion of D. villosus in western Europe, despite slight variations in allelic proportions according to spatio-temporal subdivisions of our dataset. In addition, we analysed the prevalence and diversity of parasites across its native and adopted range. We found no macro-parasites, and no significant parasite loss of microsporidian parasites during the invasive process. Our data suggest that D. villosus invasion was either massive, or recurrent, or both, allowing a parasitic cortege to follow the host. The maintenance of genetic diversity may have contributed to its success, including the variation in resistance in the face of the natural enemies.

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“…DAN and DNI populations were differentiated with a significant Fst value of 0.17. Although the invasion dynamics of the killer shrimp along the Danube and in French rivers was assessed by Wattier et al [ 19 ] based on the three microsatellite loci available at that time [ 18 ], additional loci are needed for further assessment of its expansion all over Europe. The eight new loci will be highly valuable in identifying sources of introduction for the Alpine lakes and for the UK, that are not directly connected to any of the invasion highways (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Such marker will also help to estimate the differentiation between invasion corridors and chances for putative hybridization in case the two populations originating in different areas of the native range (Danube vs. Dnieper) meet in Poland. The three already known loci [ 18 ] proved to be useful [ 19 ] but additional loci are needed to answer more detailed questions.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

et al. 2014

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Dikerogammarus villosus is a freshwater amphipod of the Ponto-Caspian origin recognized as one of the 100 worst alien species in Europe, having negative impact on biodiversity and functioning of the invaded aquatic ecosystems. The species has a wide ecophysiological tolerance and during the last 20 years it has rapidly spread throughout European inland waters. In consequence, it presents a major conservation management problem. We describe eight polymorphic microsatellite loci developed for D. villosus by combining a biotin-enrichment protocol and new generation 454GS-FLX Titanium pyrosequencing technology. When genotyped in 64 individuals from two locations, the loci exhibited a mean diversity of 4.87 alleles per locus (2–13). The mean observed and expected heterozygosities were, respectively, 0.439 (0.091–0.844) and 0.468 (0.089–0.843). Gametic disequilibrium was not detected for any pair of loci. The microsatellite markers will be a valuable tool in assessing the demographic processes associated with invasion of the killer shrimp from a genetic point of view.

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Molecular markers for systematic identification and population genetics of the invasive Ponto‐Caspian freshwater gammarid Dikerogammarus villosus (Crustacea, Amphipoda)

Cited by 9 publications

References 9 publications

Enemy release and genetic founder effects in invasive killer shrimp populations of Great Britain

Enemy release and genetic founder effects in invasive killer shrimp populations of Great Britain

No genetic bottleneck or associated microparasite loss in invasive populations of a freshwater amphipod

Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

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