Molecular markers for prediction of risk of radiation-related injury to normal tissue

Ghilotti, Marco; Pierotti, Marco A.; Gariboldi, Manuela

doi:10.4081/jnai.2010.2055

Cited by 5 publications

(11 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, high doses can cause the development of side effects, such as fatigue [ 32 ], skin irritation [ 33 ], breast swelling and the risk of cardiac disease [ 34 , 35 ]. The severity of side effects in radiation treatment is dose dependent [ 8 ]. Thus, the central challenge of radiotherapy is to maximize cancer cell killing and minimize side effects.…”

Section: Discussionmentioning

confidence: 99%

“…As an integral part of cancer management, radiation therapy is an important locoregional treatment modality, contributing to more than 50% of cancer treatment [ 6 , 7 ]. However, the efficacy of radiotherapy is limited by its own strong side effects that are usually dose dependent [ 8 , 9 ]. Due to the heterogeneity of TNBC, cells present various sensitivities to radiation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Silencing of XRCC4 increases radiosensitivity of triple-negative breast cancer cells

et al. 2019

View full text Add to dashboard Cite

Background: Radiotherapy is an important locoregional treatment, and its effect on triple-negative breast cancer (TNBC) needs to be enhanced. The aim of the present study was to investigate the potential effects of XRCC4 on radiosensitivity of TNBC. Methods: The RNAi technique was implemented to establish the TNBC stable cell line with XRCC4 knockdown. MTT assay was used to detect the effect of XRCC4 knockdown on cell proliferation. Western blot and immunohistochemistry assays were employed to identify protein expression. Colony assay was performed to detect the effect of XRCC4 knockdown on the colony formation ability of TNBC cells with radiation treatment. Comet assay was conducted to evaluate the influence of XRCC4 silencing on DNA repair activity in ionizing radiation. In addition, we performed a survival analysis based on data in TCGA database. Results: XRCC4 knockdown by lentivirus-mediated shRNA had no significant effect on proliferation of TNBC cells. Knockdown of XRCC4 could substantially increase the sensitivity of TNBC cells to ionizing radiation. The DNA damage level was detected to be increased in the XRCC4 knockdown group, indicating there was a significant repair delay in the XRCC4-deleted cells. Clinical sample analysis exhibited that there were various XRCC4 expression in different patients with TNBC. Moreover, survival analysis showed that high expression of XRCC4 was significantly associated with poor progression-free survival after radiotherapy in TNBC patients. Conclusion: Our findings suggest that XRCC4 knockdown sensitizes TNBC cells to ionizing radiation, and could be considered as a novel predictor of radiosensitivity and a promising target for TNBC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Silencing of XRCC4 increases radiosensitivity of triple-negative breast cancer cells

et al. 2019

View full text Add to dashboard Cite

show abstract

“…However, in normal cells, the respiratory pathway is the major contributor to energy (ATP) production [10,15,16,28]. Two properties of 2-DG, namely, the inhibition of glycolysis and the preferential accumulation in cancer cells, have formed the basis for further investigating the mechanism of 2-DG for its use as an antitumor agent.…”

Section: -Deoxy-d-glucose Functionmentioning

confidence: 99%

“…Therefore, significant improvement in therapeutic efficacy can be achieved only by developing effective approaches based on a comprehensive understanding of the radiobiology of the tumor and normal tissues, to selectively enhance the radiation damage in tumors while reducing the damage to normal tissues [10,11,15]. Radiotherapy is now of routine value after conservative surgery to reduce locoregional tumor recurrence.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Radiosensitivity and Chemosensitivity of Breast Cancer Cells by 2-Deoxy-D-Glucose in Combination Therapy

2012

View full text Add to dashboard Cite

Breast cancer is the most common malignancy, and it is also the major cause of cancer-related deaths of women worldwide. Breast cancer treatment involves surgery, chemotherapy, radiation therapy, or combination therapy, and novel strategies are needed to boost the oncologic outcome. The non-metabolizable glucose analogue, 2-deoxy-D-glucose (2-DG) which inhibits glucose synthesis and adenosine triphosphate production, is one of the important discoveries involving the disturbances that can be caused to the process of the metabolism. The glucose analogue, 2-DG, is known as a tumor sensitizer to irradiation (IR) and chemotherapy, which help improve the treatment rates. It enhances the cytotoxicity via oxidative stress, which is more redundant in tumor cells than in normal ones. This article provides a brief summary on studies related to 2-DG chemo-/radio-sensitization effects by combination therapy of 2-DG/IR or 2-DG/doxorubicin.

show abstract

Radiotherapy toxicity

Panchal,

Mule,

Wankhede

et al. 2024

Public Health and Toxicology Issues Drug Research, Volume 2

View full text Add to dashboard Cite

Molecular markers for prediction of risk of radiation-related injury to normal tissue

Abstract: Radiotherapy is one of the most effective methods for the treatment of cancer, but

Cited by 5 publications

References 52 publications

Silencing of XRCC4 increases radiosensitivity of triple-negative breast cancer cells

Silencing of XRCC4 increases radiosensitivity of triple-negative breast cancer cells

Enhanced Radiosensitivity and Chemosensitivity of Breast Cancer Cells by 2-Deoxy-D-Glucose in Combination Therapy

Radiotherapy toxicity

Contact Info

Product

Resources

About