Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

Abstract

Selective breeding of the Pacifi c white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identifi cation of new varieties of shrimps from their phenotypic characters is diffi cult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, fi ve microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confi rmed the results from pooled genotyping. The genotyping data for the fi ve microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identifi cation of individuals in the KH-1 group and control group reached 89%. The fi ve selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L . vannamei from other populations cultured in China.

Gene set based association analyses for the WSSV resistance of Pacific white shrimp Litopenaeus vannamei

“…1 variety, which was a new selective breeding variety in China42. Five hundred healthy shrimp, with an average body length of 6.18 ± 1.11 cm and body weight of 3.59 ± 1.57 g, were cultured in filtered sea water at 24 °C with continuous aeration in plastic tanks.…”

Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)

“…Both of the adult shrimp used for library construction and tissue distribution in this study came from a variety of shrimp named Kehai No.1 [ 14 ]. This variety was selected and bred by our laboratory cooperated with local company in Hainan province.…”

Genome-wide association study identified genes associated with ammonia nitrogen tolerance in Litopenaeus vannamei

“…Marker-assisted breeding (MAS), which enables direct selection breeding of individuals with the aid of molecular markers tightly linked to the trait, is widely used in the genetic breeding of marine animals because it is associated with high genetic stability and discrimination ( Ribaut and Hoisington, 1998 ; Lu et al, 2019 ). Yu, (2014) established a method to identify single nucleotide polymorphisms (SNPs) in L . vannamei , using high-throughput next-generation sequencing transcriptome data, 96,040 SNP markers of L .…”