Molecular markers dataset to assess the genetic diversity of oriental plane trees from historical sites in Lazio (central Italy)

Alicandri, Enrica; Vettraino, Anna Maria; Agrimi, Mariagrazia; Ciaffi, M.; Kuzminsky, Elena

doi:10.1016/j.dib.2022.108100

Cited by 1 publication

(2 citation statements)

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“…SSR primer pairs were multiplexed, labelling their forward primer with FAM, TAMRA, and JOE fluorescent dyes (Eurofins Genomics, Ebersberg, Germany). PCR reactions were performed as described previously [40], using the specific annealing temperature of each primer pair (Table S3). Amplified SSR products were genotyped using an ABI PRISM 3500 sequencer (Applied Biosystems, Waltham, MA, USA) and sized in accordance with GeneScan LI500Liz standard using Gene Mapper 4.0 software (Applied Biosystems).…”

Section: Dna Extraction and Molecular Markers (Ssr And Issr) Analysismentioning

confidence: 99%

“…For the ISSR markers, DNA samples were amplified with ten primers (Table S4), two of which were previously employed to study the genetic variability in different genotypes of the genus Platanus [41], and eight from the British Columbia University (UBC) collection, which had been used to genetically characterise different artichoke germplasm collections [9,17,19,24]. PCR reactions and the analysis of amplification products were performed according to Alicandri et al [40].…”

Section: Dna Extraction and Molecular Markers (Ssr And Issr) Analysismentioning

confidence: 99%

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Morphological, Molecular, and Nutritional Characterisation of the Globe Artichoke Landrace “Carciofo Ortano”

Alicandri

Paolacci

Catarcione

et al. 2023

Plants

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The present study focused on the molecular, morphological, and nutritional characterisation of a globe artichoke landrace at risk of genetic erosion still cultivated in the municipality of Orte (Lazio Region, Central Italy) and therefore named “Carciofo Ortano”. Molecular analysis based on SSR and ISSR markers was carried out on 73 genotypes selected at random from 20 smallholdings located in the Orte countryside and 17 accessions of landraces/clones belonging to the main varietal types cultivated in Italy. The results confirmed that “Carciofo Ortano” belongs to the “Romanesco” varietal typology and revealed the presence within the landrace of two distinct genetic populations named Orte 1 and Orte 2. Despite the high level of within-population genetic variation detected, the two populations were genetically differentiated from each other and from the landraces/clones of the main varietal types cultivated in Italy. Morphological and nutritional characterisation was performed on representative genotypes for each of the two populations of the “Carciofo Ortano” and the four landraces/clones included in the varietal platform of the PGI “CARCIOFO ROMANESCO DEL LAZIO” used as reference genotypes (“Campagnano”, “Castellammare”, “C3”, and “Grato 1”). Principal component analysis showed that, of the 43 morphological descriptors considered, 12, including plant height, head shape index, head yield, and earliness, allowed a clear grouping of genotypes, distinguishing Orte 1 and Orte 2 populations from the reference genotypes. Regarding the nutritional composition of heads, particular attention should be devoted to the Orte 2 genotypes for their high dietary fibre, inulin, flavonoid, and phenol content, a feature that could be highly appreciated by the market.

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Section: Dna Extraction and Molecular Markers (Ssr And Issr) Analysismentioning

confidence: 99%

Section: Dna Extraction and Molecular Markers (Ssr And Issr) Analysismentioning

confidence: 99%

Morphological, Molecular, and Nutritional Characterisation of the Globe Artichoke Landrace “Carciofo Ortano”

Alicandri

Paolacci

Catarcione

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

Molecular markers dataset to assess the genetic diversity of oriental plane trees from historical sites in Lazio (central Italy)

Cited by 1 publication

References 6 publications

Morphological, Molecular, and Nutritional Characterisation of the Globe Artichoke Landrace “Carciofo Ortano”

Morphological, Molecular, and Nutritional Characterisation of the Globe Artichoke Landrace “Carciofo Ortano”

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