Molecular markers, BM1500 and UMN2008, are associated with post-thaw motility of bull sperm

Alyethodi, R. R.; Deb, Rajib; Alex, Rani; Kumar, Sushil; Singh, Umesh; Tyagi, S.; Mandal, D. K.; Raja, T. V.; Das, Alok Kumar; Sengar, Gyanendra Singh; Singh, Rani; Prakash, B.

doi:10.1016/j.anireprosci.2016.10.001

Cited by 3 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In bulls, genetic diversity was positively correlated with post-thaw motility and viability [92]. In this species, some microsatellite markers and single nucleotide polymorphisms (SNPs) were found to be related to post-thaw motility [30]. No study is available investigating possible genetic effects on post-thaw canine sperm quality in the relevant literature, rendering breed-specific studies using microsatellites and SNPs highly interesting.…”

Section: Discussionmentioning

confidence: 99%

“…Further markers of sperm functionality were assessed in high-density spermatozoa, indicative of normal glycolysis, zona binding and motility [29]. Meanwhile, in this species, genome-wide association studies investigated genetic causes of poor sperm quality, and microsatellites markers (SNPs), such as BM1500 and UMN2008, were found to be related to freezability, especially post-thaw motility [30]. In rams, a strong correlation between seminal plasma composition and freezing resilience of semen has been found [31].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Canine Spermatozoa—Predictability of Cryotolerance

Schäfer-Somi

Colombo

Luvoni

2022

Animals

View full text Add to dashboard Cite

Markers of freezability allow the selection of ejaculates of good freezability. So far, most investigations were conducted in boars, bulls, rams and horses, with high economic interests triggering the efforts. The progress in dogs is comparably slow. A critical evaluation of the methods requires consideration of practicability, with most labs not even possessing a computer assisted sperm analyser (CASA); furthermore, small canine ejaculates mostly do not allow the use of large semen volumes. In dogs, modern markers of freezability no longer assess single membrane constituents or seminal plasma components but comprise tests of cell functionality and adaptability, energy metabolism, cluster analyses of kinetic and morphometric parameters, as well as DNA intactness. Identification of the most efficient combination of tests seems useful. At present, examination by CASA combined with cluster analysis of kinetic subgroups, JC-1 staining and COMET assay or staining with toluidine blue seem most appropriate; however, cell volumetry and other functional tests deserve better attention. A better understanding of spermatozoa energy metabolism might reveal new markers. This review focuses on the requirements and markers of freezability of canine semen, highlighting potential future candidates.

show abstract

Section: Discussionmentioning

confidence: 99%