Molecular markers as a method to evaluate the movement of Hypothenemus hampei (Ferrari)

Abstract

The objective of this research was to develop a methodology to describe the movement of the coffee berry borer Hypothenemus hampei (Coleoptera: Curculionidae) in the field through: (i) the evaluation of allele variation of a microsatellite marker on polymorphic Colombian H. hampei populations; (ii) the invention of a device for releasing H. hampei adults; (iii) the standardization of a release-recapture technique for H. hampei populations; (iv) the estimation of the flight distance of the insect; and (v) the calculation of a mathematical expression that describes the movement of H. hampei in space over time. The results indicated that: (i) the microsatellite molecular marker HHK.1.6 was exclusively present in a population from Guapotá-Santander, was dominant and allows the evaluation of H. hampei movement for several generations; (ii) a device that released 88.8% of H. hampei adults in 2 s was designed; (iii) this device was used as H. hampei populations containing HHK.1.6 marker release strategy, and coffee seeds as recapture strategy; (iv) it was estimated that H. hampei adults flew as far as 65 m, however, 90% were recovered in a radius of <40 m. Finally, (v) the mathematical expression that described the movement of H. hampei in space over time was Y^=αβXi, being Y^ the average number of borer beetles recaptured per tree, and x the distance in meters. This method will allow to determine the movement of H. hampei from different environmental and ecological scenarios.

Estimating the effective sampling area of an alcohol-baited trap for monitoring the coffee berry borer

“…Although coffee trees may shorten the duration of continuous flights, the dispersive capacity of the CBB appears relatively modest. For example, this beetle has been recorded infesting coffee berries on trees at 40 m from the release point after 24 h, under the same wind speed, similar to the findings in the present study (Gil et al, 2015). This corroborates laboratory observations on CBBs flying continuously for over 22 min before the first landing and displaying successive flights for up to 3 h (Baker, 1984).…”

“…This corroborates laboratory observations on CBBs flying continuously for over 22 min before the first landing and displaying successive flights for up to 3 h (Baker, 1984). Furthermore, CBBs use coffee trees as bridges to disperse through connected coffee plots (Avelino et al, 2012), thus allowing these beetles to reach tens to hundreds of meters (Baker, 1984;Gingerich et al, 1996;Mathieu et al, 1999;Gil et al, 2015). Besides, the fact that a small percentage of CBBs have been trapped at heights between 2.5 and 3.5 m (Ruiz-Diaz and Rodrigues, 2021) also indicates that these beetles can fly upwards above coffee trees from where they could likely disperse to farther distances helped by stronger wind currents.…”

Response of Coffee Berry Borer (Hypothenemus hampaei) to Alcohol-Based Attractants on Coffee Crops in Banjarnegara, Indonesia

“…Based on this concept, insects will fly to the nearest source of food conserve energy. The phenomenon of distance followed the mathematical model of the movement of H. hampei in space over time was 𝑌 = 𝛼𝛽 𝑋𝑖 , Y is the average number of insect and x is the distance in meter [13]. If x value is high then the number of insect is low and vice versa.…”

El uso de controladores naturales en estrategias de manejo integrado de plagas a gran escala

“…Otras actividades que mayor cantidad de adultos de broca dispersan son la recolección y el beneficio del café, durante el tiempo de cosecha (Castro et al, 1998;Moreno et al, 2001). En plantaciones establecidas, la broca se dispersa a máximo 65 m de distancia y el 90% de los insectos que vuelan se quedan en los frutos de los árboles localizados en los 40 m circundantes (Gil et al, 2015).…”