Molecular markers and SEM imaging reveal pseudocryptic diversity within the Ponto-Caspian low-profile amphipod invader <i>Dikerogammarus bispinosus</i>

Morhun, Halyna; Copilaş‐Ciocianu, Denis; Rewicz, Tomasz; Son, Mikhail O.; Khomenko, Andrii; Huseynov, M.; Utevsky, Serge; Grabowski, Michał

doi:10.1080/24750263.2021.2018056

Cited by 12 publications

(17 citation statements)

References 61 publications

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“…However, Pontogammarus borceae possessed three MOTUs in the Black Sea basin and one in the Caspian Sea. Our study further con rms previous studies that Dikerogammarus haemobaphes, D. bispinosus, Pontogammarus maeoticus and perhaps Chaetogammarus ischnus contain cryptic lineages that require taxonomic attention 32,33,36 . Interestingly, for amphipods, cryptic or pseudo-cryptic lineages have not yet been detected outside the native range.…”

Section: Taxonomic and Evolutionary Implicationssupporting

confidence: 89%

“…1). We generally aimed to represent each species by several sequences (mean = 5, range 1-24) from various geographical localities, preferably from both the Black Sea and Caspian Sea basins, as they are known to harbor distinct lineages 25,33,69 . Amphipods were identi ed using the appropriate keys 23,70−73 .…”

Section: Sampling and Data Collectionmentioning

confidence: 99%

“…Amphipods are by far the most speciose, with 96 endemic species currently known, spanning across a broad eco-morphological gradient 23 . Nevertheless, only a fraction of these species has been sequenced 25,27−30 , a handful have been studied in a detailed phylogeographic context [31][32][33][34][35] , and even scarcer are the modern integrative taxonomic studies 33,36 . Generally, Ponto-Caspian amphipods are poorly studied from evolutionary, ecological, and taxonomic perspectives as most studies focused on a few species of the well-known invaders 23 .…”

Section: Introductionmentioning

confidence: 99%

“…Generally, Ponto-Caspian amphipods are poorly studied from evolutionary, ecological, and taxonomic perspectives as most studies focused on a few species of the well-known invaders 23 . As a result, Ponto-Caspian amphipod taxonomy is plagued by numerous issues ranging from ambiguous species identity (including invasive ones), to uncertain species diversity at generic or even family levels 23,25,32,33 .…”

Section: Introductionmentioning

confidence: 99%

“…Using two mitochondrial markers, the standard COI barcoding region and a segment of 16S rDNA, this study further evaluates the congruence between molecular operational taxonomic units (MOTUs) and the current morphology-based taxonomy. The resulting reference library will help to disentangle the convoluted taxonomic structure of this endemic radiation if integrated with molecular species delimitation methods, morphology, and ecology 23,33,53 . A taxonomically validated reference library will bring three key bene ts.…”

Section: Introductionmentioning

confidence: 99%

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The “Crustacean seas” in the light of DNA barcoding: a reference library for endemic Ponto-Caspian amphipods

Copilaş‐Ciocianu

Rewicz

Sands

et al. 2022

Preprint

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The Ponto-Caspian region is an endemicity hotspot that harbors several crustacean radiations, among which amphipods are the most diverse. These poorly known species are severely threatened in their native range, while at the same time they are invading European inland waters with significant ecological consequences. A proper taxonomic knowledge of this fauna is paramount for its conservation within and monitoring outside the native region. Here, we assemble a DNA barcode reference library for nearly 60% of all known Ponto-Caspian amphipod species. We use several methods to define molecular operational taxonomic units (MOTUs) based on two mitochondrial markers (COI and 16S) and assess their congruence with current species-level taxonomy based on morphology. Depending on the method, we find that 54–69% of species had congruent morpho-molecular boundaries. The cases of incongruence resulted from lumping distinct morphospecies into a single MOTU (7–27%), splitting a morphospecies into several MOTUs (4–28%), or both (4–11%). MOTUs defined by distance-based methods without a priori divergence thresholds showed the highest congruence with morphological taxonomy. These results indicate that DNA barcoding is valuable for clarifying the diversity of Ponto-Caspian amphipods, but reveals that extensive work is needed to resolve taxonomic uncertainties. Our study advances the DNA barcode reference library for the European aquatic biota, paving the way towards improved taxonomic knowledge needed to enhance monitoring and conservation efforts.

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Section: Taxonomic and Evolutionary Implicationssupporting

confidence: 89%

Section: Sampling and Data Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The “Crustacean seas” in the light of DNA barcoding: a reference library for endemic Ponto-Caspian amphipods

Copilaş‐Ciocianu

Rewicz

Sands

et al. 2022

Preprint

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Conservation of freshwater biodiversity in Tunisia in a climate change context: combining amphipod distribution data and molecular analyses to improve priorities

et al. 2023

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Genetic diversity of Ponto-Caspian amphipods throughout the invaded Baltic and native NW Black Sea donor ranges: does introduction mode matter?

et al. 2023

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Molecular markers and SEM imaging reveal pseudocryptic diversity within the Ponto-Caspian low-profile amphipod invader Dikerogammarus bispinosus

Cited by 12 publications

References 61 publications

The “Crustacean seas” in the light of DNA barcoding: a reference library for endemic Ponto-Caspian amphipods

The “Crustacean seas” in the light of DNA barcoding: a reference library for endemic Ponto-Caspian amphipods

Conservation of freshwater biodiversity in Tunisia in a climate change context: combining amphipod distribution data and molecular analyses to improve priorities

Genetic diversity of Ponto-Caspian amphipods throughout the invaded Baltic and native NW Black Sea donor ranges: does introduction mode matter?

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