Molecular marker genotypes, heterozygosity and genetic interactions explain heterosis in Arabidopsis thaliana

Syed, Naeem H.; Chen, Zengjian J

doi:10.1038/sj.hdy.6800558

Cited by 56 publications

(45 citation statements)

References 51 publications

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“…Since Arabidopsis as a weed was exposed to diverse selection pressures during its evolution, one may expect that each parent harbors complexes of genes at different loci displaying positive main effects and positive interactions for growth-related traits as a major determinant of competition and reproductive fitness. This hypothesis is in agreement with the findings of Syed and Chen (2005), who concluded that heterozygosity in individual chromosomes or segments combined with epistasis may be the reason for the observed heterosis in the related cross Col 3 Ler.…”

Section: Midparent Heterosis and Genomewide Genetic Effectssupporting

confidence: 93%

“…Recent studies demonstrated a substantial amount of heterosis for biomass yield and growth-related traits with a wide variation among individual hybrids (Barth et al 2003;Meyer et al 2004). First, QTL analyses on heterosis in cross Col 3 Ler were performed with testcross progenies of RILs using a TTC design (Kearsey et al 2003) and design III (Syed and Chen 2005). A detailed analysis of heterosis for biomass-related traits in the cross C24 3 Col-0 was conducted in a previous study (Kusterer et al 2007a) and in an accompanying study (Kusterer et al 2007b, this issue) with TTC progenies of RILs.…”

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confidence: 99%

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Genetic Basis of Heterosis for Growth-Related Traits in Arabidopsis Investigated by Testcross Progenies of Near-Isogenic Lines Reveals a Significant Role of Epistasis

et al. 2007

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Epistasis seems to play a significant role in the manifestation of heterosis. However, the power of detecting epistatic interactions among quantitative trait loci (QTL) in segregating populations is low. We studied heterosis in Arabidopsis thaliana hybrid C24 3 Col-0 by testing near-isogenic lines (NILs) and their triple testcross (TTC) progenies. Our objectives were to (i) provide the theoretical basis for estimating different types of genetic effects with this experimental design, (ii) determine the extent of heterosis for seven growth-related traits, (iii) map the underlying QTL, and (iv) determine their gene action. Two substitution libraries, each consisting of 28 NILs and covering $61 and 39% of the Arabidopsis genome, were assayed by 110 single-nucleotide polymorphism (SNP) markers. With our novel generation means approach 38 QTL were detected, many of which confirmed heterotic QTL detected previously in the same cross with TTC progenies of recombinant inbred lines. Furthermore, many of the QTL were common for different traits and in common with the 58 QTL detected by a method that compares triplets consisting of a NIL, its recurrent parent, and their F 1 cross. While the latter approach revealed mostly (75%) overdominant QTL, the former approach allowed separation of dominance and epistasis by analyzing all materials simultaneously and yielded substantial positive additive 3 additive effects besides directional dominance. Positive epistatic effects reduced heterosis for growth-related traits in our materials.

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Section: Midparent Heterosis and Genomewide Genetic Effectssupporting

confidence: 93%

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confidence: 99%

Genetic Basis of Heterosis for Growth-Related Traits in Arabidopsis Investigated by Testcross Progenies of Near-Isogenic Lines Reveals a Significant Role of Epistasis

et al. 2007

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“…The additive QTL do not contribute to heterosis, while the non-additive QTL, including dominant, overdominant, and epistatic do contribute [12,15,19,22,24,25,28]. The second one is to use the measurements of heterosis as the data input to perform QTL analysis [13][14][15][24][25][26]30,43]. QTL effects for Z 1 and Z 2 in the RIL-based NCIII design provide estimates of the augmented effects a*, which measures the net contribution of the QTL to the parental difference, and d*, which measures the net contribution of the QTL to midparent heterosis [14].…”

Section: Discussionmentioning

confidence: 99%

“…Heterosis represents one of the most revolutionary advancements in crop improvement, i.e., maize [8] and rice [4]. In the genetic dissection of heterosis, NCIII design, proposed by Comstock and Robinson [9,10], is one of the most powerful and widely used mating schemes [11][12][13][14][15][16][17]. Therefore, the importance of heterosis necessitates an in-depth study of the NCIII design.…”

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confidence: 99%

Genome-wide mapping of QTL associated with heterosis in the RIL-based NCIII design

Zhang

2012

Chin. Sci. Bull.

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Heterosis represents one of the most revolutionary advancements in crop improvement. In the genetic dissection of heterosis, NCIII design is one of the most powerful and widely used mating schemes. However, the methodologies for quantitative trait loci (QTL) detection in the design were mostly based on composite interval mapping. Therefore, in this study, our purpose was to develop a statistical method for mapping epistatic QTL associated with heterosis in the RIL-based NCIII design. First, we derivated the expectations of two classical linear transformations, Z 1 and Z 2 , while a quantitative trait was controlled by two QTL with digenic epistasis and arbitrary linkage under the F ∞ and F 2 metric models. Then, we constructed an epistatic genetic model that includes all markers on the whole genome simultaneously, and estimated all the parameters in the model by the empirical Bayes approach. Finally, a series of Monte Carlo simulation experiments was carried out to confirm the proposed approach. The results show that: (1) all the augmented genetic parameters for main-effect QTL could be rightly identified with satisfactory statistical power and precision; (2) the statistical powers in the detection of augmented epistatic effects were substantively affected by the signs of pure epistatic effects; (3) it is more difficult to detect epistatic QTL than to detect main-effect QTL; (4) statistical power is higher in the RIL-based NCIII design than in the F 2 -based NCIII design, especially in the detection of the augmented epistatic effect that consists of two pure epistatic effects in opposite directions.empirical Bayes, epistasis, NCIII design, heterosis, quantitative trait locus, recombinant inbred line Citation:He X H, Hu Z L, Zhang Y M. Genome-wide mapping of QTL associated with heterosis in the RIL-based NCIII design.

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“…Heterosis, or hybrid vigor, refers to the phenomenon that the progeny of diverse inbred varieties exhibit greater biomass and speed of development than the better of the two parents; however, the molecular mechanism of heterosis remains to be elucidated (Xu et al, 2005;Xie et al, 2006). The development of quantitative trait locus mapping has facilitated the investigation of heterosis (Syed and Chen, 2005). However, the phenomenon of heterosis is controlled by gene expression and regulation in the heterozygote (Xu et al, 2005;Liu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and functional analysis of MRLC2 in Tianfu, Boer, and Chengdu Ma goats

Xu¹,

Xu²,

Lu³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.To determine the molecular basis of heterosis in goats, fluorescence quantitative polymerase chain reaction (PCR) was performed to investigate myosin-regulatory light chain 2 (MRLC2) gene expression in the longissimus dorsi muscle tissues of the Tianfu goat and its parents, the Boer and Chengdu Ma goats. The goat MRLC2 gene was differentially expressed in the crossbreed, and the purebred mRNA were isolated and identified using fluorescence quantitative reverse transcription-PCR (RT-PCR). The complete coding sequence of MRLC2 was obtained using the cDNA method, and the full-length coding sequence consisted of 513 bp encoding 172 amino acids. The EF-hand superfamily domain of the MRLC2 protein is well conserved in caprine and other animals. The deduced amino acid sequence of MRLC2 shared significant identity with MRLC2 from other mammals. Phylogenetic tree analysis revealed that the MRLC2 protein was closely related to MRLC2 in other mammals. Several predicted miRNA target sites were found in the coding sequence of caprine MRLC2 mRNA. Analysis by RT-PCR showed that MRLC2 mRNA was present in the ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (3): 3510-3520 (2013) Function analysis of the MRLC2 gene 3511 heart, stomach, liver, spleen, lung, small intestine, kidney, leg muscle, abdominal muscle, and longissimus dorsi muscles. In particular, the high expression of MRLC2 mRNA was detected in the longissimus dorsi, leg muscle, abdominal muscle, stomach, and heart, but low levels of expression were also observed in the liver, spleen, lung, small intestine, and kidney. The expression of the MRLC2 gene was upregulated in the longissimus dorsi muscle of Boer and Tianfu goats, and it was moderately upregulated in Chengdu Ma goats.

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Molecular marker genotypes, heterozygosity and genetic interactions explain heterosis in Arabidopsis thaliana

Cited by 56 publications

References 51 publications

Genetic Basis of Heterosis for Growth-Related Traits in Arabidopsis Investigated by Testcross Progenies of Near-Isogenic Lines Reveals a Significant Role of Epistasis

Genetic Basis of Heterosis for Growth-Related Traits in Arabidopsis Investigated by Testcross Progenies of Near-Isogenic Lines Reveals a Significant Role of Epistasis

Genome-wide mapping of QTL associated with heterosis in the RIL-based NCIII design

Molecular cloning and functional analysis of MRLC2 in Tianfu, Boer, and Chengdu Ma goats

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