Molecular marker for characterization of traditional and hybrid derivatives of Eleusine coracana (L.) using ISSR marker

Abstract

Background Finger millet is the most important food grain in the world for its nutritional benefits. Finger millet is genetically and geographically diverse and widely spread in the African and Asian sub-continent. Therefore, the present study was undertaken to analyze the genetic diversity using ISSR genetic markers using 15 ISSR primers. Results About 23 genotypes of widely cultivated finger millet cultivars of economically important ones were characterized and the ISSR markers were critically analyzed for their performance with parameters such as polymorphic information content (PIC), effective multiplex ratio (EMR), marker index (MI), and resolving power (RP). In this study, 175 loci were scored across the 23 cultivars of finger millet, and out of these 173 loci (98%) were polymorphic, revealing the suitability of these loci for genetic diversity analysis with ISSR marker. The average number of polymorphic loci per primer was 11.50 with varying sizes from 100 bp to 2500 bp. ISSR primers that showed higher polymorphism were found to have higher EMR and MI values up to 15.30 and 13.44, respectively. Conclusion High degree of polymorphism supported with distinct differences of all the marker parameters revealed the suitability of ISSR markers for determining the genotypic differences based on ISSR markers among the 23 genotypes of finger millet. The possible application of the ISSR marker in the conservation and management of finger millet genetic resources is discussed.

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

“…The possible reasons are its center of origin of the plant and anthropogenic activities followed by unrestricted plant material transfer within the geographical boundaries. The obtained results were also supported by similar trend of results are reported in many plant species concerning different geographical locations (Rajendran et al 2016;Kelkar et al 2017;Zhou et al 2020;Venkatesan et al 2021). This type of unrestricted genetic resources migration within and between geographical boundaries may enhance the adaptability of the species in harsh climates leading to the development of better genotypes.…”

Molecular Diversity in Populations of Chili (Capsicum Annuum L.)

“…Resolving power (R) is the foremost vital parameter to decide the discriminatory efficiency of the primers in the hybrid derivates of Eleusine coracana (Venkatesan et al, 2021). The average R was 6.6047, lower than 10.74 in the Moroccan argan tree (Pakhrou et al, 2020).…”

Analysis of population structure and genetic diversity of Iranian Wild Salicornia (Salicornia iranica Akhani) population

“…These researchers mentioned that for achieving high diversity in populations Salicornia, a wider research scope is required to be chosen [16]. Using ISSR markers to identify genotypic differences among the 23 genotypes of finger millet revealed a high degree of polymorphism supported by substantial differences in all marker parameters [33].…”