Molecular-marker-based Genetic Analysis of Tepary Bean-derived Common Bacterial Blight Resistance in Different Developmental Stages of Common Bean

Abstract

<jats:p>Randomly amplified polymorphic DNA (RAPD) molecular markers were used to construct a partial genetic linkage map in a recombinant inbred population derived from the common bean (<jats:italic>Phaseolus vulgaris</jats:italic> L.) cross PC-50 × XAN-159 for studying the genetics of bacterial disease resistance in common bean. The linkage map spanned 426 cM and included 168 RAPD markers and 2 classical markers with 11 unassigned markers. The seventy recombinant inbred lines were evaluated for resistance to two strains of common bacterial blight [<jats:italic>Xanthomonas campestris</jats:italic> pv. <jats:italic>phaseoli</jats:italic> (Smith) Dye] (<jats:italic>Xcp</jats:italic>). Common bacterial blight (CBB) resistance was evaluated for <jats:italic>Xcp</jats:italic> strain EK-11 in later-developed trifoliolate leaves and for <jats:italic>Xcp</jats:italic> strains, DR-7 and EK-11, in first trifoliolate leaves, seeds, and pods. One to four quantitative trait loci (QTLs) accounted for 18% to 53% of the phenotypic variation for traits. Most significant effects for CBB resistance were associated with one chromosomal region on linkage group 5 and with two regions on linkage group 1, of the partial linkage map. The chromosomal region (a 13-cM interval) in linkage group 5 was significantly associated with resistance to <jats:italic>Xcp</jats:italic> strains DR-7 and EK-11 in leaves, pods, and seeds. The regions in linkage group 1 were also significantly associated with resistance to both <jats:italic>Xcp</jats:italic> strains in more than one plant organ. In addition, a seedcoat pattern gene (<jats:italic>C</jats:italic>) and a flower color gene (<jats:italic>v<jats:sup>lae</jats:sup></jats:italic>) were mapped in linkage groups 1 and 5, respectively, of the partial linkage map. The <jats:italic>V</jats:italic> locus was found to be linked to a QTL with a major effect on CBB resistance.</jats:p>

75 years of breeding pinto bean for resistance to diseases in the United States

“…As we know now there are more than 20 major and minor effect QTL controlling resistance to common bacterial blight that are distributed over the common bean genome (see review by Kelly et al 2003 andMiklas andSingh 2006). Although heritability of resistance to common bacterial blight is much higher than that for resistance to white mold, tight undesirable linkages between common bacterial blight resistance and seed coat and flower color (Jung et al 1997;Mutlu et al 2005;Park et al 1999) have limited the utility of resistance genes/ QTL such that no cultivars with high level of resistance are popular (Table 1).…”

Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding

“…Symbols in subscript represent the source population of the QTL. AG: A55/G 122 , BA: Belneb-RR-1/A55 Fourie et al, 2004;Jung et al, 2003), BE: Berna/EMP 419 (Murray et al, 2004a,b), BG: BAT 881/G 21212 (Frei et al, 2005), BJ: BAT 93/Jalo EEP558 (Freyre et al, 1998;Gepts, 1999;Geffroy et al, 2000), BH: BAC 6/HT 7719 (Jung et al, 1996), BN: Bunsi/Newport (Kolkman & Kelly, 2003), BR: Bunsi/Raven (Ender & Kelly, 2005), B60: Benton/NY6020-4 (Miklas et al, 2003b), DG: DOR 364/G 19833 (López et al, 2003), DX: DOR 364/XAN 176 (Miklas et al, 2000c), H95: HR67/OAC 95 (Yu et al, 2004), MF: Montcalm/FR266 (Schneider et al, 2001), PX: PC50/XAN 159 (Jung et al, 1997(Jung et al, , 1998Park et al, 2001), RN: Red Hawk/Negro San Luis (Román-Avilés & , S95: Seaforth/OAC 95 (Tar'an et al, 2001), and XC: XR-235-1-1/Calima (Yu et al, 1998). Gene and QTL locations are approximate because most were not directly mapped in the BAT 93/Jalo EEP558 population.…”

“…Breeding for genetic resistance is complex as revealed by identification of 22 QTL distributed across all 11 chromosomes (Figure 1). Expression of these QTL is influenced by environment, disease pressure, plant maturity and plant organ: seed, leaf, and pod Jung et al, 1997;Miklas et al, 1996b;Santos et al, 2003; reviewed by Kelly &Kelly et al, 2003).…”

“…Translocation could explain the movement of traits from the end of one linkage group B6 to the end of another B7. Location of BC420 to linkage group B6 was based primarily on linkage with V locus that conditions purple flower color and dark seed colors (Jung et al, 1997). The integration of V may be misplaced or the gene influencing seed color linked with BC420 may also derive from tepary bean and occur at a different locus than V. Nonetheless, repeated attempts to recover the resistance QTL linked with BC420 marker in seed types other than white, black, or black-mottled has failed.…”

Genetic mapping of quantitative resistance to race 5 of Pseudomonas syringae pv. phaseolicola in common bean

“…Several types of molecular markers, such as RAPD, AFLP, SSR, ISSR and RFLP, have been used to obtain linkage maps in Phaseolus vulgaris (Adam-Blondon et al 1994;Ariyarathne et al 1999;Blair et al 2003;Freyre et al 1998;Jung et al 1996Jung et al , 1997Nodari et al 1993;Pedrosa et al 2003;Santos et al 2003;Tar'an et al 2002;Vallejos et al 1992). RAPD are the most commonly employed markers in this set of papers, probably due to their low cost and easy handling.…”