Molecular marker based detection of leaf rust resistance gene Lr34 in Indian bread wheat (Triticum aestivum L.)

Abstract

Leaf rust is one of the most destructive diseases of wheat causing huge economic losses throughout the world. Several leaf rust resistance genes have been identified and genetically mapped, some of which have also been cloned including the adult plant resistance gene Lr34. Selection of wheat genotypes carrying Lr34 can be accomplished with the help of expression of a morphological marker leaf tip necrosis (LTN) which is linked with Lr34. However, recording of LTN under field conditions is time and labor intensive thereby slowing down the process of selection of desirable genotypes. Use of molecular markers which are tightly linked to Lr34 can be more efficient in precise identification of lines carrying this gene in a short span of time. We selected a total of seven such markers that were earlier reported to be linked with Lr34. The objective of the study was to assess the suitability of these markers in distinguishing Indian bread wheat genotypes carrying Lr34 from those which lacked Lr34. The haplotype analysis suggested that one STS marker (csLV34) and two gene specific markers (cssfr2 and cssfr5) could distinguish wheat genotypes carrying Lr34 from those lacking it. Use of these markers in breeding program can accelerate the speed of selection of desirable genotypes.

Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection

“…Fungicides are the most favored method by breeders to fight fungal diseases, but they are expensive and can harm the environment [ 19 ]. Genetic resistance breeding is an efficient, economical and environmentally friendly way to relieve pathogen damage [ 20 ], saving time and effort compared to traditional methods [ 21 ].…”

Development of multiplex PCR to detect slow rust resistance genes Lr34 and Lr46 in wheat

“…There are many fungicides that help control these fungal disease, but their use is expensive and can have a negative impact on the environment. The most efficient, economical, and environmentally sound method to mitigate the losses caused by pathogens is breeding for genetic resistance (Muthe et al 2016). To date, more than 70 leaf rust resistance ( Lr ) genes have been cataloged (McIntosh et al 2013), but many of these genes are race-specific and they have lose their effectiveness when new races of the pathogen are appearing (McCallum et al 2007).…”