Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower

Berry, Simon; Leόn, Alberto J.; Hanfrey, C. C.; Challis, P.; Bürkholz, Alexandra; Barnes, Stephen R.; Rufener, G. K.; Lee, M.; Caligari, P. D. S.

doi:10.1007/bf00220877

Cited by 125 publications

(88 citation statements)

References 26 publications

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“…org/supplemental/). Linkages were labeled according to the standard nomenclature for H. annuus (Berry et al 1995;Gedil et al 2001). The relationship between this nomenclature and that of Rieseberg et al (1995Rieseberg et al ( , 1999) is shown in Burke et al (2004) and Rieseberg et al (2003).…”

Section: Resultsmentioning

confidence: 99%

Extensive Chromosomal Repatterning and the Evolution of Sterility Barriers in Hybrid Sunflower Species

et al. 2005

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New species may arise via hybridization and without a change in ploidy. This process, termed homoploid hybrid speciation, is theoretically difficult because it requires the development of reproductive barriers in sympatry or parapatry. Theory suggests that isolation may arise through rapid karyotypic evolution and/or ecological divergence of hybrid neospecies. Here, we investigate the role of karyotypic change in homoploid hybrid speciation by generating detailed genetic linkage maps for three hybrid sunflower species, Helianthus anomalus, H. deserticola, and H. paradoxus, and comparing these maps to those previously generated for the parental species, H. annuus and H. petiolaris. We also conduct a quantitative trait locus (QTL) analysis of pollen fertility in a BC 2 population between the parental species and assess levels of pollen and seed fertility in all cross-combinations of the hybrid and parental species. The three hybrid species are massively divergent from their parental species in karyotype; gene order differences were observed for between 9 and 11 linkage groups (of 17 total), depending on the comparison. About one-third of the karyoypic differences arose through the sorting of chromosomal rearrangements that differentiate the parental species, but the remainder appear to have arisen de novo (six breakages/six fusions in H. anomalus, four breakages/three fusions in H. deserticola, and five breakages/five fusions in H. paradoxus). QTL analyses indicate that the karyotypic differences contribute to reproductive isolation. Nine of 11 pollen viability QTL occur on rearranged chromosomes and all but one map close to a rearrangement breakpoint. Finally, pollen and seed fertility estimates for F 1 's between the hybrid and parental species fall below 11%, which is sufficient for evolutionary independence of the hybrid neospecies.

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Section: Resultsmentioning

confidence: 99%

Extensive Chromosomal Repatterning and the Evolution of Sterility Barriers in Hybrid Sunflower Species

et al. 2005

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“…The first genetic linkage map for sunflower was actually developed for a wild hybrid species, H. anomalus (Rieseberg et al 1993), which is divergent chromosomally from the domesticated sunflower (Rieseberg et al 1995). However, shortly thereafter several restriction fragment length polymorphism maps were published for the domesticated sunflower (Berry et al 1995;Gentzbittel et al 1995Gentzbittel et al , 1999Jan et al 1998), establishing a standard nomenclature for sunflower linkage groups and providing framework markers for comparisons among maps (Gedil et al 2001). More recently, high-resolution linkage maps based on more than 1,000 simple sequence repeat (SSR) and other sequence-tagged-site markers have been developed for the cultivated sunflower Yu et al 2003) and several wild species Burke et al 2004;Lai et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Identification and mapping of SNPs from ESTs in sunflower

et al. 2005

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More than 67,000 expressed sequence tags (ESTs) have recently been generated for sunflower (Helianthus), including 44,000 from cultivated confectionery (RHA280) and oilseed (RHA801) lines of Helianthus annuus and 23,000 from droughtand salt-tolerant wild sunflowers, H. argophyllus and H. paradoxus, respectively. To create a transcript map for sunflower, we identified 605 ESTs that displayed small insertion-deletion polymorphism (SNP) variation in silico, had apparent tissuespecific expression patterns, and/or were ESTs with candidate functions in traits such as development, cell transport, metabolism, plant defense, and tolerance to abiotic stress. Primer pairs for 535 of the loci were designed from the ESTs and screened for polymorphism in recombinant inbred lines derived from a cross between the same cultivars (RHA280 × RHA801) employed for sequencing. In total, 273 of the loci amplified polymorphic products, of which 243 mapped to the 17 linkage groups previously identified for sunflower. Comparisons with previously mapped QTL revealed some cases where ESTs with putatively related functions mapped near QTLs identified in other crosses for salt tolerance and for domestication traits such as stem diameter, shattering, flowering time, and achene size.

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“…Given that as few as five backcrosses can regenerate 98.5% of the original nuclear complement in CMS lines of H. annuus (Fick 1978), a survey of 20 loci is probably insufficient to reveal any introgression. This suggests that the precise genetic contribution of other wild species to the genome of the ancestral domesticate will probably remain a mystery unless a large number of nuclear markers such as restriction fragment length polymorphisms (RFLPs) (Berry et al 1995) or RAPDs (Rieseberg et al 1993) are assayed from the genome of H. annuus and close relatives such as H. petiolaris.…”

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confidence: 99%

Allozyme variation in domesticated annual sunflower and its wild relatives

et al. 1997

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The annual sunflower (Helianthus annuus L.) is a morphologically and genetically variable species composed of wild, weedy, and domesticated forms that are used for ornament, oilseed, and edible seeds. In this study, we evaluated genetic variation in 146 germplasm accessions of wild and domesticated sunflowers using allozyme analysis. Results from this survey showed that wild sunflower exhibits geographically structured genetic variation, as samples from the Great Plains region of the central United States were genetically divergent from accessions from California and the southwestern United States. Sunflower populations from the Great Plains harbored greater allelic diversity than did wild sunflower from the western United States. Comparison of genetic variability in wild and domesticated sunflower by principal coordinate analysis showed these groups to be genetically divergent, in large part due to differences in the frequency of common alleles. Neighbor-Joining analyses of domesticated H. annuus, wild H. annuus and two closely related wild species (H. argophyllus T. & G. and H. petiolaris Nutt.) showed that domesticated sunflowers form a genetically coherent group and that wild sunflowers from the Great Plains may include the most likely progenitor of domesticated sunflowers. Keywords Helianthus annuus, Allozymes, Crop evolution, Genetic diversity Disciplines Botany | Genetics | Plant Breeding and Genetics CommentsThis article is from Theoretical and Applied Genetics 95 (1997): 532, doi:10.1007/s001220050594. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

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Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower

Cited by 125 publications

References 26 publications

Extensive Chromosomal Repatterning and the Evolution of Sterility Barriers in Hybrid Sunflower Species

Extensive Chromosomal Repatterning and the Evolution of Sterility Barriers in Hybrid Sunflower Species

Identification and mapping of SNPs from ESTs in sunflower

Allozyme variation in domesticated annual sunflower and its wild relatives

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