Molecular marker aided characterization of race specific and non-race specific rust resistance genes in elite wheat (Triticum spp.) germplasm

Pal, Dharam; Kumar, Subodh; Bhardwaj, S. C.; Harikrishna,; Devate, Narayana Bhat; Patial, Madhu; Parmeshwarappa, Sunil Kumar V.

doi:10.1007/s13313-022-00850-3

Cited by 3 publications

(2 citation statements)

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“…The first wheat variety containing Lr24 was released in 1993 and since then, a number of varieties were released with the Lr24 gene ( Tomar et al., 2014 ). Hence, the presence of Lr24 in Indian wheat varieties is extremely useful for resistance against leaf rust ( Pal et al., 2022 ). In recent years, new virulent races have appeared against some leaf rust resistance genes ( Prasad et al., 2019 ) among which 77-9 and 77-5 are most prevalent and virulent.…”

Section: Discussionmentioning

confidence: 99%

Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086

Sunilkumar

Krishna²,

Devate³

et al. 2022

Front. Plant Sci.

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There is a significant yield reduction in the wheat crop as a result of different biotic and abiotic stresses, and changing climate, among them moisture deficit stress and leaf rust are the major ones affecting wheat worldwide. HD3086 is a high-yielding wheat variety that has been released for commercial cultivation under timely sown irrigated conditions in the Indo-Gangetic plains of India. Variety HD3086 provides a good, stable yield, and it is the choice of millions of farmers in India. It becomes susceptible to the most prevalent pathotypes 77-5 and 77-9 of Puccinia triticina (causing leaf rust) in the production environment and its potential yield cannot be realized under moisture deficit stress. The present study demonstrates the use of a marker-assisted back cross breeding approach to the successful transfer of leaf rust resistance gene Lr24 and QTLs linked to moisture deficit stress tolerance in the background of HD3086. The genotype HI1500 was used as a donor parent that possesses leaf rust-resistant gene Lr24, which confers resistance against the major pathotypes found in the production environment. It possesses inbuilt tolerance under abiotic stresses with superior quality traits. Foreground selection for gene Lr24 and moisture deficit stress tolerance QTLs linked to Canopy temperature (CT), Normal Differential Vegetation Index (NDVI) and Thousand Kernel Weight (TKW) in different generations of the backcrossing and selection. In BC2F2, foreground selection was carried out to identify homozygous lines based on the linked markers and were advanced following pedigree based phenotypic selection. The selected lines were evaluated against P. triticina pathotypes 77-5 and 77-9 under controlled conditions. Recurrent parent recovery of the selected lines ranged from 78-94%. The identified lines were evaluated for their tolerance to moisture stress under field conditions and their resistance to rust under artificial epiphytotic conditions for two years. In BC2F5 generation, eight positive lines for marker alleles were selected which showed resistance to leaf rust and recorded an improvement in component traits of moisture deficit stress tolerance such as CT, NDVI, TKW and yield compared to the recurrent parent HD3086. The derived line is named HD3471 and is nominated for national trials for testing and further release for commercial cultivation.

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Section: Discussionmentioning

confidence: 99%

Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086

Sunilkumar

Krishna²,

Devate³

et al. 2022

Front. Plant Sci.

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“…The use of molecular markers in wheat breeding is a fast and efficient way to confirm and detect the presence of resistance genes. They have an edge over the standard phenotyping as these are not affected by plant growth stages or the environment [62]. In the present study, DNA markers were used to postulate the presence of Yr2, Yr5, Yr7, Yr9, Yr10, Yr15, Yr17, Yr18, Yr24, Yr25 and Yr60.…”

Section: Discussionmentioning

confidence: 98%

Resistance of Modern Russian Winter Wheat Cultivars to Yellow Rust

Gultyaeva,

Shaydayuk

2023

Plants

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Over the last decade, the significance of yellow rust caused by Puccinia striiformis (Pst) has substantially increased worldwide, including in Russia. The development and cultivation of resistant genotypes is the most efficient control method. The present study was conducted to explore the yellow rust resistance potential of modern common winter wheat cultivars included in the Russian Register of Breeding Achievements in 2019–2022 using the seedling tests with an array of Pst races and molecular markers linked with Yr resistance genes. Seventy-two winter wheat cultivars were inoculated with five Pst isolates differing in virulence and origin. Molecular markers were used to identify genes Yr2, Yr5, Yr7, Yr9, Yr10, Yr15, Yr17, Yr18, Yr24, Yr25 and Yr60. Thirteen cultivars were resistant to all Pst isolates. The genes Yr5, Yr10, Yr15 and Yr24 that are effective against all Russian Pst races in resistant cultivars were not found. Using molecular methods, gene Yr9 located in translocation 1BL.1RS was detected in 12 cultivars, gene Yr18 in 24, gene Yr17 in 3 and 1AL.1RS translocation with unknown Yr gene in 2. While these genes have lost effectiveness individually, they can still enhance genetic diversity and overall yellow rust resistance, whether used in combination with each other or alongside other Yr genes.

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The Role of Genetic, Genomic, and Breeding Approaches in the Fight Against Fungal Diseases in Wheat

Kumar

Choudhary

Sharma

et al. 2022

Cereal Diseases: Nanobiotechnological Approaches for Diagnosis and Management

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Molecular marker aided characterization of race specific and non-race specific rust resistance genes in elite wheat (Triticum spp.) germplasm

Cited by 3 publications

References 50 publications

Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086

Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086

Resistance of Modern Russian Winter Wheat Cultivars to Yellow Rust

The Role of Genetic, Genomic, and Breeding Approaches in the Fight Against Fungal Diseases in Wheat

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