Molecular mapping of QTLs for fiber quality traits in Gossypium hirsutum multi-parent recombinant inbred lines

Baytar, Asena Akköse; Peynircioğlu, Ceng; Sezener, Volkan; Frary, Anne; Doğanlar, Sami

doi:10.1007/s10681-021-02914-9

Cited by 2 publications

(1 citation statement)

References 88 publications

(98 reference statements)

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“…Cotton is one of the most important cash crops, which supplies raw material to more than 50 industries globally and is extensively planted in more than 70 countries throughout the world [ 1 ]. Of the four cultivated species, upland cotton ( Gossypium hirsutum L.), a main supplier for the textile industry, contributes approximately 95% of cotton production, owing to its high yield, attractive fiber quality, and wide adaptability [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study of Lint Percentage in Gossypium hirsutum L. Races

Wang

Guo

Cai

et al. 2023

IJMS

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Lint percentage is one of the most essential yield components and an important economic index for cotton planting. Improving lint percentage is an effective way to achieve high-yield in cotton breeding worldwide, especially upland cotton (Gossypium hirsutum L.). However, the genetic basis controlling lint percentage has not yet been systematically understood. Here, we performed a genome-wide association mapping for lint percentage using a natural population consisting of 189 G. hirsutum accessions (188 accessions of G. hirsutum races and one cultivar TM-1). The results showed that 274 single-nucleotide polymorphisms (SNPs) significantly associated with lint percentage were detected, and they were distributed on 24 chromosomes. Forty-five SNPs were detected at least by two models or at least in two environments, and their 5 Mb up- and downstream regions included 584 makers related to lint percentage identified in previous studies. In total, 11 out of 45 SNPs were detected at least in two environments, and their 550 Kb up- and downstream region contained 335 genes. Through RNA sequencing, gene annotation, qRT-PCR, protein–protein interaction analysis, the cis-elements of the promotor region, and related miRNA prediction, Gh_D12G0934 and Gh_A08G0526 were selected as key candidate genes for fiber initiation and elongation, respectively. These excavated SNPs and candidate genes could supplement marker and gene information for deciphering the genetic basis of lint percentage and facilitate high-yield breeding programs of G. hirsutum ultimately.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study of Lint Percentage in Gossypium hirsutum L. Races

Wang

Guo

Cai

et al. 2023

IJMS

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Genetic analysis of heat tolerance in hot pepper: insights from comprehensive phenotyping and QTL mapping

TS,

Srivastava,

Tomar

et al. 2023

Front. Plant Sci.

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High temperatures present a formidable challenge to the cultivation of hot pepper, profoundly impacting not only vegetative growth but also leading to flower and fruit abscission, thereby causing a significant reduction in yield. To unravel the intricate genetic mechanisms governing heat tolerance in hot pepper, an F2 population was developed through the crossing of two distinct genotypes exhibiting contrasting heat tolerance characteristics: DLS-161-1 (heat tolerant) and DChBL-240 (heat susceptible). The F2 population, along with the parental lines, was subjected to comprehensive phenotyping encompassing diverse morphological, physiological, and biochemical heat-related traits under high temperature conditions (with maximum temperature ranging from 31 to 46.5°C and minimum temperature from 15.4 to 30.5°C). Leveraging the Illumina Nova Seq-6000 platform, Double digest restriction-site associated DNA sequencing (ddRAD-seq) was employed to generate 67.215 Gb data, with subsequent alignment of 218.93 million processed reads against the reference genome of Capsicum annuum. Subsequent variant calling and ordering resulted in 5806 polymorphic SNP markers grouped into 12 LGs. Further QTL analysis identified 64 QTLs with LOD values ranging from 2.517 to 11.170 and explained phenotypic variance ranging from 4.05 to 19.39%. Among them, 21 QTLs, explaining more than 10% phenotypic variance, were identified as major QTLs controlling 9 morphological, 3 physiological, and 2 biochemical traits. Interestingly, several QTLs governing distinct parameters were found to be colocalized, suggesting either a profound correlation between the QTLs regulating these traits or their significant genomic proximity. In addition to the QTLs, we also identified 368380 SSR loci within the identified QTL regions, dinucleotides being the most abundant type (211,381). These findings provide valuable insights into the genetics of heat tolerance in hot peppers. The identified QTLs and SSR markers offer opportunities to develop heat-tolerant varieties, ensuring better crop performance under high-temperature conditions.

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Molecular mapping of QTLs for fiber quality traits in Gossypium hirsutum multi-parent recombinant inbred lines

Cited by 2 publications

References 88 publications

Genome-Wide Association Study of Lint Percentage in Gossypium hirsutum L. Races

Genome-Wide Association Study of Lint Percentage in Gossypium hirsutum L. Races

Genetic analysis of heat tolerance in hot pepper: insights from comprehensive phenotyping and QTL mapping

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