Molecular Mapping of PMR1, a Novel Locus Conferring Resistance to Powdery Mildew in Pepper (Capsicum annuum)

Jo, Jinkwan; Venkatesh, Jelli; Han, Koeun; Lee, Hea-Young; Choi, Gyung Ja; Lee, Hee Jae; Choi, Doil; Kang, Byoung−Cheorl

doi:10.3389/fpls.2017.02090

Cited by 29 publications

(11 citation statements)

References 53 publications

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“…Nucleotide-binding site leucine-rich repeat (NBS-LRR) genes constitute the largest family of R genes. NBS-LRR genes are associated with resistance to powdery and downy mildew pathogens in several species, including Arabidopsis (Warren et al 1998), wheat (He et al 2018), barley (Wei et al 1999; Zhou et al 2001), pepper (Jo et al 2017), and grapes (Riaz et al 2011; Zini et al 2019). We divided the NBS-LRR genes into eight different classes depending on the domains detected in the proteins: CC-NBS-LRR, CC-NBS, CC-X, TIR-NBS-LRR, TIR-NBS, TIR-X, NBS-LRR, NBS (called hereafter NBS-LRR when described as a whole).…”

Section: Resultsmentioning

confidence: 99%

Diploid chromosome-scale assembly of theMuscadinia rotundifoliagenome supports chromosome fusion and disease resistance gene expansion duringVitisandMuscadiniadivergence

Cochetel

Minio

Vondras

et al. 2020

Preprint

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Muscadinia rotundifolia, the muscadine grape, has been cultivated for centuries in the southeastern United States. M. rotundifolia is resistant to many of the pathogens that detrimentally affect Vitis vinifera, the grape species commonly used for winemaking throughout Europe and in New World wine regions. For this reason, M. rotundifolia is a valuable genetic resource for breeding. Single molecule real-time reads were combined with optical maps to reconstruct the two haplotypes of each of the 20 M. rotundifolia cv. Trayshed (Trayshed, henceforth) chromosomes. Completeness and accuracy of the assembly was confirmed using a high-density linkage map of M. rotundifolia. Protein-coding genes were annotated using an integrated comprehensive approach that included full-length cDNA sequencing (Iso-Seq) to improve gene structure and hypothetical spliced variant predictions. Our data confirmed the fusion of chromosomes 7 and 20, which reduced the number of chromosomes in Vitis versus Muscadinia and pinpointed the location of the fusion in Cabernet Sauvignon and PN40024 chromosome 7. The numbers of nucleotide binding site leucine-rich repeats (NBS-LRR) in Trayshed and Cabernet Sauvignon were similar, but their locations were different. A dramatic expansion of the Toll/Interleukin-1 Receptor-like-X (TIR-X) class was detected on Trayshed chromosome 12 at the Resistance to Uncinula necator 1 (RUN1)/ Resistance to Plasmopara viticola 1 (RPV1) locus, which confers strong dominant resistance to powdery and downy mildew. A genome browser for Trayshed, its annotation, and an associated Blast tool are available at www.grapegenomics.com.

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Section: Resultsmentioning

confidence: 99%

Diploid chromosome-scale assembly of theMuscadinia rotundifoliagenome supports chromosome fusion and disease resistance gene expansion duringVitisandMuscadiniadivergence

Cochetel

Minio

Vondras

et al. 2020

Preprint

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“…Necrotic and chlorotic leaf spotting reported by Jørgensen (1992) is similar to the HR characteristics observed in this study. In an investigation into accumulation of H 2 O 2 because of loss-of-function of mlo gene, Piffanelli et al (2002) reported a (Jo et al, 2017). It would be worthwhile to screen the evaluated material in this study with the PMR1 marker.…”

Section: Discussionmentioning

confidence: 97%

Identification of Resistance to Powdery Mildew in Chile Pepper

McCoy¹,

Bosland²

2019

horts

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Powdery mildew [Leveillula taurica (L ev.) Arn] is a fungus causing epidemics on chile peppers (Capsicum sp.) worldwide. It was first observed in New Mexico in the late 1990s and has been a reoccurring issue. During the 2017 growing season, environmental conditions were highly favorable for powdery mildew development and severe infection was observed. This provided a unique opportunity to identify novel sources of resistance in Capsicum to powdery mildew. In the present study, the incidence and severity of powdery mildew was evaluated for 152 chile pepper accessions comprising different cultivars and species. Major differences in disease severity and incidence were observed among the accessions. Of the 152 accessions, 53 were resistant, i.e., received a disease index (DI) score of £1. When examining across Capsicum species, 16 Capsicum annuum accessions, all 8 Capsicum baccatum, all 21 Capsicum chinense, 5 of 6 Capsicum frutescens, the Capsicum chacoense accession, and the Capsicum rhomboideum accession were resistant. These results provide several accessions with resistance that can be used in breeding programs. Especially important are the C. annuum resistant accessions, as this resistance can be more quickly incorporated into commercially important C. annuum cultivars as compared with interspecific hybridizations.

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“…The first attempt to map powdery mildew resistance was carried out by Lefebvre and colleagues, which described the quantitative nature of the resistance identifying a robust Quantitative Trait Locus (QTL) on chromosome 6 (Lt 6.1), and reported epistatic interactions explaining more than 50% of the genotypic variance [18] (Table 2). A single dominant locus, PMR1, located in a syntenic region of 4 Mb of pepper Chr 4 and responsible for the powdery mildew resistance has been reported [19]. Within this locus, two genes across the 622 predicted ones were found to share sequence similarity to the nucleotide-binding site leucine-rich repeat domain containing R proteins (NBS-LRR), which represent clusters of resistance genes in plants.…”

Section: Bacterial Spot Of Peppermentioning

confidence: 99%

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

Parisi¹,

Alioto

Tripodi³

2020

IJMS

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Pepper (Capsicum spp.) is one of the major vegetable crops grown worldwide largely appreciated for its economic importance and nutritional value. This crop belongs to the large Solanaceae family, which, among more than 90 genera and 2500 species of flowering plants, includes commercially important vegetables such as tomato and eggplant. The genus includes over 30 species, five of which (C. annuum, C. frutescens, C. chinense, C. baccatum, and C. pubescens) are domesticated and mainly grown for consumption as food and for non-food purposes (e.g., cosmetics). The main challenges for vegetable crop improvement are linked to the sustainable development of agriculture, food security, the growing consumers’ demand for food. Furthermore, demographic trends and changes to climate require more efficient use of plant genetic resources in breeding programs. Increases in pepper consumption have been observed in the past 20 years, and for maintaining this trend, the development of new resistant and high yielding varieties is demanded. The range of pathogens afflicting peppers is very broad and includes fungi, viruses, bacteria, and insects. In this context, the large number of accessions of domesticated and wild species stored in the world seed banks represents a valuable resource for breeding in order to transfer traits related to resistance mechanisms to various biotic stresses. In the present review, we report comprehensive information on sources of resistance to a broad range of pathogens in pepper, revisiting the classical genetic studies and showing the contribution of genomics for the understanding of the molecular basis of resistance.

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Molecular Mapping of PMR1, a Novel Locus Conferring Resistance to Powdery Mildew in Pepper (Capsicum annuum)

Cited by 29 publications

References 53 publications

Diploid chromosome-scale assembly of theMuscadinia rotundifoliagenome supports chromosome fusion and disease resistance gene expansion duringVitisandMuscadiniadivergence

Diploid chromosome-scale assembly of theMuscadinia rotundifoliagenome supports chromosome fusion and disease resistance gene expansion duringVitisandMuscadiniadivergence

Identification of Resistance to Powdery Mildew in Chile Pepper

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

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