Molecular mapping of locus controlling resistance to Helicoverpa armigera (Hubner) in Cajanus cajan L. (Millspaugh) using interspecific F2 mapping population

Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

“…In F3 generation, the segregation pattern of 78 F3 families for plume moth reaction was good fit for 1 (18 non segregating resistant): 2 (46 segregating): 1 (14 non segregating susceptible) ratio (χ 2 = 2.923, p-value = 0.403), evidencing monogenic segregation pattern and single dominant gene action, as reported for pod borer resistance (Verulkar et al, 1997;Aruna et al, 2005;Mishra et al, 2013) and Fusarium wilt resistance (Kotresh et al, 2006) in pigeon-pea. This monogenic segregation for plume moth resistance was further established by 1:1 segregation pattern at BC1F1 (χ 2 = 0.332, p-value = 0.564) lines.…”

“…Maesen possessed with host resistance to pod borer (H. armigera) and some of its allied pests including pod fly, blue butterfly and plume moth (Sujana et al, 2008;Panigrahi, 2011). Hence, the genetic control of resistance to these pests have been identified in pigeon-pea using interspecific populations involving accessions of C. cajan and C. scarabaeoides (Verulkar et al, 1997;Aruna et al, 2005;Mishra et al, 2013;Sahu et al, 2015). All earlier workers reported that, resistance to pod borer (caused by H. armigera) is controlled by dominant allele of a single gene.…”

“…The random amplified polymorphic DNA (RAPD) markers (Williams et al, 1990) coupled with bulked segregant analysis (BSA; Michelmore et al, 1991) has been used for molecular tagging and mapping of genes controlling agronomically important traits in various crops including pigeon-pea (Kotresh et al, 2006;Ganapathy et al, 2009;Dhanasekar et al, 2010;Mishra et al, 2013). Recent days, RAPD markers are not favored in breeding endeavors due to its inconsistency in amplification across the laboratories.…”