Molecular Mapping of <i>YrSP</i> and Its Relationship with Other Genes for Stripe Rust Resistance in Wheat Chromosome 2BL

Feng, Jiayi; Wang, M N; Chen, X M; See, Deven R.; Zheng, Yuzhi; Chao, Shiaoman; Wan, Anmin

doi:10.1094/phyto-03-15-0060-r

Cited by 38 publications

(26 citation statements)

References 40 publications

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“…We selected SNP markers by "linkage map walking" on the distal and proximal side of the target region, and the initial linkage map was constructed with eight SNPs and qualitative disease reaction data to map Yr5 on the chromosome arm 2BL in two RIL populations. Moreover, Yr53 is flanked by wmc149 and wmc441 (Xu et al, 2013) where IWA4866 and IWA6121 are located at the distal side of the gene (Feng et al, 2015). Yr7, Yr43, Yr44, Yr53, and YrSP are race-specific allstage resistance genes, in addition to Yr5, and are located on the long arm of chromosome 2B (Zhang et al, 2009;Cheng and Chen, 2010;Xu et al, 2013;Feng et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The KASP markers based on SNP loci IWA6121 and IWA4096 were identified as flanking markers for Yr5 in both RIL populations. Yr7, Yr43, Yr44, Yr53, and YrSP are race-specific allstage resistance genes, in addition to Yr5, and are located on the long arm of chromosome 2B (Zhang et al, 2009;Cheng and Chen, 2010;Xu et al, 2013;Feng et al, 2015). These Yr genes are mapped to a similar position as Yr5 on an integrated consensus map (Maccaferri et al, 2015), though they are either defeated by contemporary PNW races or are from exotic sources.…”

Section: Discussionmentioning

confidence: 99%

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Identification and Validation of SNP Markers Linked to the Stripe Rust Resistance Gene Yr5 in Wheat

et al. 2016

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Stripe rust (Puccinia striiformis Westend f. sp. tritici) is a devastating disease for wheat (Triticum aestivum L.) production worldwide. Yr5 is a race‐specific resistance gene, effective to all races that have been identified in the United States. Therefore, it has been increasingly used for US cultivar development. The goal of this study was to identify and validate single‐nucleotide polymorphism (SNP) markers tightly linked with Yr5, evaluate their usefulness with a diverse set of wheat materials, and dissect the Yr5 region to search for candidate genes using a comparative genomic approach. Competitive allele‐specific polymerase chain reaction (KASP) markers based on Illumina iSelect 9000 SNP loci IWA6121 and IWA4096 were identified as flanking markers for Yr5 in two spring wheat recombinant inbred line populations that were developed from crosses between moderately susceptible line ‘WA8149’ and Yr5 donors ‘S0900317’ and ‘S0900163.’ These markers were closer to the gene than previously reported markers. Haplotype analysis based on loci of these KASP markers in a set of the Pacific Northwest spring and winter wheat lines and a worldwide spring wheat core collection showed that the combined haplotype for these markers is nearly diagnostic for the presence of Yr5. The Yr5‐syntenic regions in Brachypodium and rice (Oryza sativa L.) contained a putative gene encoding a nucleotide binding site–leucine‐rich repeat disease resistance protein. Wheat gene Traes_2BL_B5FC3BC9E showed high similarity with these Brachypodium and rice genes, and a marker designed from this gene was mapped closest to Yr5 in the recombinant inbred line populations. This study provides useful SNP markers for Yr5 in wheat breeding programs and resources for potential map‐based cloning of Yr5.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification and Validation of SNP Markers Linked to the Stripe Rust Resistance Gene Yr5 in Wheat

et al. 2016

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“…As Yr76 is race-specific, it should not be used alone. Therefore, combinations of this gene with other widely effective all-stage resistance genes such as Yr5, Yr15, Yr45, Yr53, Yr61, Yr64, Yr65, and YrSP (Chen et al 2003;Feng et al 2015;Li et al 2010;Murphy et al 2009;Xu et al 2013;Zhou et al 2014a), and/or with genes for HTAP resistance such as Yr36, Yr39, Yr52, Yr59, Yr62, and many temporarily named QTL (Carter et al 2009;Chen 2013;Chen 2007, 2009;Lu et al 2014;Ren et al 2012;Uauy et al 2005;Zhou et al 2014b) are recommended.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mapping of Stripe Rust Resistance Gene Yr76 in Winter Club Wheat Cultivar Tyee

et al. 2016

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Tyee, one of the wheat cultivars used to differentiate races of Puccinia striiformis f. sp. tritici in the United States, was identified to have a single gene for all-stage resistance, tentatively named YrTye. To map the gene, Tyee was crossed with ‘Avocet Susceptible’ (AvS). Genetic analysis of the F1, F2, F2:3, and BC1 progenies confirmed a single dominant gene for resistance to race PSTv-37 that is avirulent to YrTye. A mapping population of 135 F2 plants was phenotyped with PSTv-37 and the derived F2:3 lines were tested with races PSTv-37, PSTv-40, and PSTv-79. The F2 mapping population was genotyped with simple sequence repeat (SSR) markers. A genetic map comprising 13 SSR markers located YrTye in chromosome 3AS flanked distally by SSR marker wmc11 and proximally by wmc532 at 2.6 and 3.4 cM, respectively. Amplification of Chinese Spring 3A deletion lines placed the gene in the distal bin 3AS4-0.45 to 1.00. Because YrTye is different from all formally named Yr genes in chromosomal location, we permanently name the gene Yr76. A near-isogenic line of spring common wheat was developed and selected by testing F3 lines derived from a AvS*4/Tyee cross with Tyee-avirulent and virulent races and the flanking markers. The specific SSR alleles flanking Yr76 were validated using cultivars and breeding lines with and without the gene, and showed high polymorphisms. The specificity of Yr76 is useful in differentiating P. striiformis f. sp. tritici races, and its tightly linked markers will be useful in developing resistant cultivars when combining the gene with other genes for resistance to stripe rust.

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“…Further assessment on the presence or absence of this gene in Ethiopian durum wheat may help to decide whether or not to use it in wheat breeding program. YrSp, originally identified from ' Spaldings Prolific ' wheat provides resistance to a broad spectrum of Pst races and is mapped on 2BL [57]. Recently, Marchal et al [40] identified that YrSp is allelic to Yr5 and is a truncated version of Yr5 sharing 99.8% sequence in common.…”

Section: Kasp Assay Based Screening For Known Yr Genesmentioning

confidence: 99%

Identification of Stripe Rust Resistance in Ethiopian Durum Wheat by Phenotypic Screening and Kompetitive Allele Specific PCR (KASP) SNP Markers

Alemu¹,

Badebo²,

Tesfaye³

et al. 2019

J Plant Pathol Microbiol

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Stripe (Yellow) rust caused by Puccinia striiformis f.sp. tritici (Pst) is one of the most devastating diseases of wheat in the highlands of Ethiopia. Improved cultivars often lose their resistance due to occurrence of new virulent races which overcome the genes and make the cultivars out of production. Therefore, identification of new sources of resistance genes helps in battling yellow rust and maximizes wheat production in Ethiopia. In this study, 300 durum wheat lines (landraces & cultivars) were screened with three virulent isolates (Pst_Is1, Pst_Is4 and Pst_Is8) for seedling resistance using Infection Type (IT) scoring method. The lines were also screened with 16 KASP-based SNP markers linked to 7 Yr genes already identified in various studies. Highly resistant infection type (IT: 0-3) to Pst_Is1, Pst_Is4, and Pst_Is8 was exhibited by 59.3%; 67.3%; and 46.3% of the lines, respectively. 124 lines constantly exhibited high level of resistance to all three isolates. The majority (96.8%) of the resistant lines are landraces while four (3.2%) are commercial cultivars (Cocorit/71, Yerer, Obsa and Dire). In the molecular screening 12 of the markers gave clear amplifications in the controls and the tested lines. Yr7, Yr15 and YrSp were detected in 81.7%, 88.3% and 0.7% of the lines respectively while Yr1, Yr17 and Yr36 were not detected. Detection frequency was higher in landraces (58.7%) than in cultivars (32.8%). Gene combinations frequency was the highest (72.7%) for Yr7+Yr15 followed by Yr15+YrSp (0.3%). Overall, this study has resulted in detection of genes Yr15 and YrSp, which are potential candidates for marker assisted breeding for Pst resistance in wheat. Besides, it has shown that resistant source identification and detection of genes can be facilitated through combined application of phenotyping and molecular screening.

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Molecular Mapping of YrSP and Its Relationship with Other Genes for Stripe Rust Resistance in Wheat Chromosome 2BL

Cited by 38 publications

References 40 publications

Identification and Validation of SNP Markers Linked to the Stripe Rust Resistance Gene Yr5 in Wheat

Identification and Validation of SNP Markers Linked to the Stripe Rust Resistance Gene Yr5 in Wheat

Molecular Mapping of Stripe Rust Resistance Gene Yr76 in Winter Club Wheat Cultivar Tyee

Identification of Stripe Rust Resistance in Ethiopian Durum Wheat by Phenotypic Screening and Kompetitive Allele Specific PCR (KASP) SNP Markers

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