Molecular Mapping of Disease Resistance Genes for Halo Blight, Common Bacterial Blight, and Bean Common Mosaic Virus in a Segregating Population of Common Bean

Abstract

<jats:p>Diseases of beans (<jats:italic>Phaseolus vulgaris</jats:italic> L.) are primary constraints affecting bean production. Information on tagging and mapping of genes for disease resistance is expected to be useful to breeders. The objectives of this study were to develop a random amplified polymorphic DNA (RAPD) marker linkage map using 78 F9 recombinant inbred (RI) lines derived from a Middle-American common bean cross Great Northern Belneb RR-1 [resistant to common bacterial blight (CBB) and halo blight (HB)] × black A 55 [dominant <jats:italic>I</jats:italic> gene resistance to bean common mosaic potyvirus] and to map genes or QTL (quantitative trait loci) for resistance to CBB, HB, BCMV (bean common mosaic virus), and BCMNV (bean common mosaic necrosis virus) diseases. The RI lines were evaluated for resistance to leaf and pod reactions to <jats:italic>Xanthomonas campestris</jats:italic> pv. <jats:italic>phaseoli</jats:italic> (<jats:italic>Xcp</jats:italic>) (Smith Dye) strain EK-11, leaf reactions to two <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>phaseolicola</jats:italic> (<jats:italic>Psp</jats:italic>) (Burkholder) Young et al. (1978) strains HB16 and 83-Sc2A, and BCMV strain US-5 and BCMNV strain NL-3. The linkage map spanned 755 cM, including 90 markers consisting of 87 RAPD markers, one sequence characterized amplified region (SCAR), the <jats:italic>I gene</jats:italic>, and a gene for hypersensitive resistance to HB 83-Sc2A. These were grouped into 11 linkage groups (LG) corresponding to the 11 linkage groups in the common bean integrated genetic map. A major gene and QTL for leaf resistance to HB were mapped for the first time. Three QTL for leaf reactions to HB16 were found on linkage groups 3, 5, and 10. Four regions on linkage groups 2, 4, 5, and 9, were significantly associated with leaf reactions to HB strain 83-Sc2A. The gene controlling the hypersensitive reaction to HB 83-Sc2A mapped to the same region as the QTL on LG 4. The <jats:italic>I</jats:italic> locus for resistance to BCMV and BCMNV was mapped to LG 2 at about 1.4 cM from RAPD marker A10.1750. Five and four markers were significantly associated with QTL for resistance to CBB in leaves and pods, respectively, with four of them associated with resistance in both plant organs. A marker locus was discovered on LG 10, W10.550, which could account for 44% and 41% of the phenotypic variation for CBB resistance in leaves and pods, respectively. QTL for resistance in pod to CBB, leaf resistance to HB, and the <jats:italic>I</jats:italic> gene were linked on LG 2.</jats:p>

Genetic mapping of quantitative resistance to race 5 of Pseudomonas syringae pv. phaseolicola in common bean

“…1). In the map this locus would be included within the LOD 2.0 interval confidence of the QTL, so it is likely that both approaches would indicate the same locus, in a similar way to the results of Ariyarathne et al (1999) for the locus Hb-7 and the QTL HB 83 located in the quoted B4 linkage group. Therefore, it could be possible that group B4 in these articles corresponds to our VII group.…”

“…Several types of molecular markers, such as RAPD, AFLP, SSR, ISSR and RFLP, have been used to obtain linkage maps in Phaseolus vulgaris (Adam-Blondon et al 1994;Ariyarathne et al 1999;Blair et al 2003;Freyre et al 1998;Jung et al 1996Jung et al , 1997Nodari et al 1993;Pedrosa et al 2003;Santos et al 2003;Tar'an et al 2002;Vallejos et al 1992). RAPD are the most commonly employed markers in this set of papers, probably due to their low cost and easy handling.…”

Counteracting virulence mechanisms of grain legume pathogens

“…Gene-for-gene Alzate-Marin et al (2000,2001,2003), Ansari et al (2004), Balardin and Kelly (1998) Macrophomina phaseolina QTL Miklas et al (1998) Uromyces appendiculatus Gene-for-gene Alzate-Marin et al (2004), Correa et al (2000), Miklas and Stavely (1998), Miklas et al (2000), Park et al (2003), Rivkin et al (1999) Bacterial diseases Common bacterial blight QTL Ariyarathne et al (1999) Jung et al (1997) Park et al (1998) Xanthomonas axonopodis pv. phaseoli (= Xanthomonas campestris pv.…”

“…phaseolicola (Halo blight) QTL Ariyarathne et al (1999) Phaseolotoxin Patil et al (1974), Turner and Mitchell (1985) Nematode diseases…”

Common Bean ( Phaseolus vulgaris L.)

“…Resistance found in common bean is controlled by one major QTL (Ariyarathne et al, 1999;Miklas et al, 1996) and that which is introgressed from tepary bean is determined by two major QTL (Pedraza et al, 1997;Yu et al, 2000). In addition, five to eight QTL with small effects determine inheritance of common bacterial blight resistance (Jung et al, 1996;Nodari et al, 1993b).…”