Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

Abstract

Background Lesion mimics (LMs) are disease-like symptoms that occur randomly on plant green leaves in the absence of pathogens. A previous study showed that LMs are related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can substantially reduce the quality and yield of crops. The development of resistant cultivars is an economical and environmentally friendly way to enhance the adaptability and yield stability of crops instead of the use of fungicide applications. Results In this study, a novel LM gene affording Pst resistance was identified and mapped with molecular markers developed for marker-assisted selection (MAS)-based wheat breeding. In this study, a novel LM gene named lm4, which is closely linked (8.06 cM) to SSR markers Xgwm210 and Xgwm455, was identified by using a Yanzhan 1/Neixiang 188 RIL population. The genetic distance of lm4 was then narrowed such that it was flanked by SSR markers with 0.51 cM and 0.77 cM intervals. Two SSR markers, lm4_01_cib and lm4_02_cib, were developed based on the content in the Chinese Spring genome database and wheat 660 K SNP results; these markers can be used to conduct MAS of LMs in wheat. The results also showed that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions Therefore, lm4 is associated with stripe rust resistance, which may provide theoretical support for future crop disease-resistance breeding and for understanding the plant apoptosis mechanism.

Multi-Layer Biosensor for Pre-Symptomatic Detection of Puccinia strifformis, the Causal Agent of Yellow Rust

“…The assembled sensor ( Figure 15 a) was inoculated with spores of the yellow rust pathogen P. s. f. sp. tritici and placed in a temperature-controlled incubator at 7 °C with a humidity of 60% in total darkness for 24 h [ 70 ]. P. s. f. sp.…”

Natural products of pentacyclic triterpenoids: from discovery to heterologous biosynthesis

“…Currently, there are mainly six TF families reported to be related to terpenoid metabolism in plants, including AP2/ERF, bHLH, WRKY, bZIP, MYB and NAC (NAM, ATAF1/2, and CUC2). 229 Among them, AP2/ERF, 230–232 bHLH, 202,233–235 WRKY, 227,236–238 bZIP, 239–241 and MYB 242 have been reported to mainly regulate the biosynthesis of plant triterpenoids.…”

Genome-Wide Characterization of HSP90 Gene Family in Malus sieversii and Their Potential Roles in Response to Valsa mali Infection

“…To analyse differentially expressed genes (DEG) of M. sieversii in response to V. mali infection, the PacBio and Illumina transcriptome sequencing data (NCBI BioProject: PR-JNA687214) were used [27]. For the sampling of the PacBio and Illumina sequencing, barks of twigs in M. sieverisii near the canker were separately harvested at the time points of 0, 1, 2, and 5 dpi, and each sample contained three biological replicates.…”

“…To understand how M. sieversii responds to the V. mali infection at a transcriptional level, we previously sequenced transcriptome of M. sieversii in response to V. mali infection by PacBio approach [27]. The KEGG pathway enrichment analysis indicated that the plant-pathogen interaction pathway was significantly enriched by the V. mali infection (Figure S1).…”