Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22

Wang, Cuifen; Guo, Yin; Xia, Xianchun; He, Zhonghu; Zhang, Peipei; Yao, Zhanjun; JinYan, Qin; Li, Zaifeng; Liu, Daqun

doi:10.1007/s11032-016-0437-3

Cited by 25 publications

(27 citation statements)

References 29 publications

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“…The cultivar of winter wheat (Triticum aestivum L.) used in this study (both the common garden and field experiment) was Zhoumai 22, which is a hybrid of Zhoumai 12, Wenmai 6, and Zhoumai 13, and developed by the Zhoukou Academy of Agricultural Sciences, Zhoukou city, Henan Province, China. This cultivar is widely grown on the central plains of China, such as the Yellow and Huaihe River basins, because of its strong cold tolerance and disease resistance [52].…”

Section: Study Materialsmentioning

confidence: 99%

Effect of Different Combinations of Phosphorus and Nitrogen Fertilization on Arbuscular Mycorrhizal Fungi and Aphids in Wheat

Chao

Tian

et al. 2020

Insects

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While chemical fertilizers can be used to increase crop yield, the abuse of fertilizers aggravates environmental pollution and soil degradation. Understanding the effects of chemical fertilizers on the interaction between arbuscular mycorrhizal fungi (AMF) and pest insects is of great benefit to crop and environmental protection, because AMF can enhance the nutrition absorption and insect resistance of crops. This study tested the effect of different levels of phosphorus, nitrogen, and their interactions on AMF, secondary metabolites, Sitobion avenae in garden, as well as the wheat traits in field. The results showed that AMF colonization on roots in the P0N1 treatment (0 g P/pot, 1.3083 g N/pot in the garden, and 0 g P/plot, 299.84 g N/plot) was the highest in both the garden and the field. The abundance of aphid was reduced in the P0N1 treatment, and there were negative relationships between aphids and AMF and phenolics, but a positive relationship between AMF and phenolics. Our results indicated that a change in the ratio of phosphorus to nitrogen affects the relationship among AMF, aphid abundance, and metabolites. The results also suggested an approach to save chemical fertilizers that could improve crop health and protect the agroecosystem against pollution at the same time.

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Section: Study Materialsmentioning

confidence: 99%

Effect of Different Combinations of Phosphorus and Nitrogen Fertilization on Arbuscular Mycorrhizal Fungi and Aphids in Wheat

Chao

Tian

et al. 2020

Insects

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“…Seven leaf rust resistance genes (Lr13, Lr16, Lr23, Lr35, Lr48, Lr73, and LrZH22) had been mapped on wheat chromosome 2BS (Seyfarth et al, 1999;Park et al, 2014;Nsabiyera et al, 2016;Wang et al, 2016;Zhang et al, 2016;Chhetri et al, 2017;Kassa et al, 2017). We found that LrLC10 is located in the region 157,688,415-158,002,717 bp (Figure 3) (2BS1-0.53-0.75) on the reference sequence of Chinese Spring (RefSeq v1.0).…”

Section: Discussionmentioning

confidence: 92%

“…We found that LrLC10 is located in the region 157,688,415-158,002,717 bp (Figure 3) (2BS1-0.53-0.75) on the reference sequence of Chinese Spring (RefSeq v1.0). Of those 7 Lr genes, only Lr13 and LrZH22 were reported to be located in the same region as LrLC10 (Wang et al, 2016;Zhang et al, 2016). LrZH22 confers resistance to leaf rust at both the seedling and adult stages (Wang et al, 2016), whereas resistance gained from LrLC10 is effective only after the four-leaf stage.…”

Section: Discussionmentioning

confidence: 99%

Fine Mapping of the Wheat Leaf Rust Resistance Gene LrLC10 (Lr13) and Validation of Its Co-segregation Markers

Qiu

Wang

et al. 2020

Front. Plant Sci.

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Wheat leaf rust, caused by the fungus Puccinia triticina Eriks. (Pt), is a destructive disease found throughout common wheat production areas worldwide. At its adult stage, wheat cultivar Liaochun10 is resistant to leaf rust and the gene for that resistance has been mapped on chromosome 2BS. It was designated LrLC10 and is the same gene as cataloged gene Lr13 by pedigree analysis and allelism test. We fine-mapped it using recessive class analysis (RCA) of the homozygous susceptible F 2 plants derived from crosses using Liaochun10 as the resistant, male parent. Taking advantage of the re-sequencing data of Liaochun10 and its counterpart susceptible parent, we converted nucleotide polymorphisms in the LrLC10 interval between the resistant and susceptible parents into molecular markers to saturate the LrLC10 genetic linkage map. Four indel markers were added in the 1.65 cM map of LrLC10 flanked by markers CAUT163 and Lseq22. Thirty-two recombinants were identified by those two markers from the 984 F 2 homozygous susceptible plants and were further genotyped with additional ten markers. LrLC10 was finally placed in a 314.3 kb region on the Chinese Spring reference sequence (RefSeq v1.0) that contains three high confidence genes: TraesCS2B01G182800, TraesCS2B01G182900, and TraesCS2B01G183000. Sequence analysis showed several variations in TraesCS2B01G182800 and TraesCS2B01G183000 between resistant and susceptible parents. One KASP marker and an indel marker were designed based on the differences in those two genes, respectively, and were validated to be diagnostic co-segregating markers for LrLC10. Our results both improve markerassisted selection and help with the map-based cloning of LrLC10.

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“…There have been many studies on temperature sensitive R genes against stripe rust, especially non-race-specific HTAP resistance, such as Yr36 (Fu et al, 2009 ; Bryant et al, 2014 ); Yr52 (Ren et al, 2012 ); Yr59 (Zhou et al, 2014 ), Yr62 (Lu et al, 2014 ), LrZH22 (Wang et al, 2016 ), and Yr79 (Feng et al, 2018 ). Previously cloned non-race-specific resistance genes to stripe rust and/or leaf rust, such as Yr18/Lr34 (Krattinger et al, 2009a ), Yr36 (Fu et al, 2009 ), and Yr46/Lr67 (Moore et al, 2015 ), do not contain NBS-LRR domains (Chen, 2013 ), in contrast to many race-specific resistance to stripe rust and leaf rust, such as Lr10 (Feuillet et al, 2003 ), Lr21 (Huang et al, 2003 ); Lr1 (Cloutier et al, 2007 ); Yr10 (Liu W. et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveal the Molecular Mechanisms of Wheat Higher-Temperature Seedling-Plant Resistance to Puccinia striiformis f. sp. tritici

et al. 2018

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Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat worldwide. The disease is preferably controlled by growing resistant cultivars. Wheat cultivar Xiaoyan 6 (XY 6) has been resistant to stripe rust since its release. In the previous studies, XY 6 was found to have higher-temperature seedling-plant (HTSP) resistance. However, the molecular mechanisms of HTSP resistance were not clear. To identify differentially expressed genes (DEGs) involved in HTSP resistance, we sequenced 30 cDNA libraries constructed from XY 6 seedlings exposed to several temperature treatments. Compared to the constant normal (15°C) and higher (20°C) temperature treatments, 1395 DEGs were identified in seedlings exposed to 20°C for 24 h (to activate HTSP resistance) and then kept at 15°C. These DEGs were located on all 21 chromosomes, with 29.2% on A, 41.1% on B and 29.7% on D genomes, by mapping to the Chinese Spring wheat genome. The 1395 DEGs were enriched in ribosome, plant-pathogen interaction and glycerolipid metabolism pathways, and some of them were identified as hub proteins (phosphatase 2C10), resistance protein homologs, WRKY transcription factors and protein kinases. The majority of these genes were up-regulated in HTSP resistance. Based on the differential expression, we found that phosphatase 2C10 and LRR receptor-like serine/threonine protein kinases are particularly interesting as they may be important for HTSP resistance through interacting with different resistance proteins, leading to a hypersensitive response.

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Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22

Cited by 25 publications

References 29 publications

Effect of Different Combinations of Phosphorus and Nitrogen Fertilization on Arbuscular Mycorrhizal Fungi and Aphids in Wheat

Effect of Different Combinations of Phosphorus and Nitrogen Fertilization on Arbuscular Mycorrhizal Fungi and Aphids in Wheat

Fine Mapping of the Wheat Leaf Rust Resistance Gene LrLC10 (Lr13) and Validation of Its Co-segregation Markers

Transcriptomic Analysis Reveal the Molecular Mechanisms of Wheat Higher-Temperature Seedling-Plant Resistance to Puccinia striiformis f. sp. tritici

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