Molecular Mapping of a Gene Conferring Fusarium Wilt Resistance in Lentil (Lens culinaris Medikus subsp. culinaris) using Bulked-segregant Analysis

Abstract

Background: Vascular wilt caused by Fusarium oxysporum f.sp. lentis Vasu. and Srini. is a serious disease of lentil (Lens culinaris Medikus), causes severe yield losses worldwide. For effective disease resistance breeding the inheritance and mapping of wilt resistance gene (s) is necessary. Therefore, the present investigation was focused on study the mode of inheritance and tag/map gene (s) for fusarium wilt resistance in lentil. Methods: Bulked segregant analysis (BSA) approach was used to identify markers that were tightly linked to Fusarium wilt resistance gene. The inheritance and mapping of wilt-resistance gene (s) in lentil was investigated in F2 and F2:3 populations derived from L9-12×ILL10965 cross, whereas L9-12 and ILL10965 were susceptible and resistant parents, respectively. Result: More than two hundreds SSRs markers were surveyed for the parental polymorphism, of which twenty nine were found polymorphic. These polymorphic SSRs were used for the bulked-segregant analysis (BSA) using both parents and its respective resistant and susceptible bulks, and three SSRs viz. PBALC233, PBALC1409 and PBALC203 could distinguish the respective bulks. Linkage analysis showed two SSR markers, PBALC203 and PBALC1409 flanking the wilt resistance gene at 8.2 cM and 9.4 cM distance, respectively. Further, PBLAC233 was also found present on the same linkage group at a distance of 10.2 cM from PBLAC1409.

Evaluation of F2 Mapping Population for Stem Rot Tolerance in Tossa Jute (Corchorus olitorius)

“…Further, the factors of climate change and availability of alternate hosts have resulted in shifting of disease and pest status in jute and allied fibre crops. The mechanism of host resistance offers the most effective way to manage the disease (Meena et al, 2021). However, none of the cultivable jute varieties has shown complete resistance against M. phaseolina (Mahapatra et al, 1994, Mandal et al, 2000, Kar et al, 2009, Srivastava et al, 2011.…”