Molecular Mapping of 2 Environmentally Sensitive Male-Sterile Mutants in Soybean

Abstract

In soybean [Glycine max (L.) Merr.], manual cross-pollination to produce large quantities of hybrid seed is difficult and time consuming. Identification of an environmentally stable male-sterility system could make hybrid seed production commercially valuable. In soybean, 2 environmentally sensitive male-sterile, female-fertile mutants (ms8 and msp) have been identified. Inheritance studies showed that sterility in both mutants is inherited as a single gene. The objectives of this study were to 1) confirm that msp and ms8 are independent genes; 2) identify the soybean chromosomes that contain the msp and the ms8 genes using bulked segregant analyses (BSAs); and 3) make a genetic linkage map of the regions containing these genes. Mapping populations consisting of 176 F(2) plants for ms8 and 134 F(2) plants for msp were generated. BSA revealed that Sat_389 and Satt172 are closely associated markers with ms8 and msp, respectively. Map location of Sat_389 suggested that the ms8 gene is located on chromosome 7; molecular linkage group (MLG) M. Map location of Satt172 indicated that the msp gene is located on chromosome 2 (MLG Dlb). Genetic linkage maps developed using F(2) populations revealed that ms8 is flanked by a telomere and Sat_389 and msp is flanked by Sat_069 and GMES4176. The region between the telomere and Sat_389 is physically 160 Kb. Soybean sequence information revealed that there are 13 genes present in that region. Protein BLASTP analyses revealed that homologs of 3 of the 13 genes are known to a play role in cell division, suggesting putative candidates for ms8.

Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean

“…Physical maps of the soybean chromosomes showing locations of all known male-sterile, female-fertile genes. The length of the bar next to the gene name represents the physical region where the gene is located (Jin et al 1998;Cervantes-Martinez et al 2007, 2009Frasch et al 2011;Ott et al 2013). The putative location of the ms7 gene is determined based on a transposon tagging study (J. Gosh, M.K.…”

“…Although abnormalities occurred throughout the development of sporogenous tissue, abnormalities peaked near pachytene stage of meiosis (Stelly and Palmer 1982). The msp gene was mapped to molecular linkage group D1b (Frasch et al 2011).…”

“…However, if plants are male-sterile, female-fertile, the manual removal of anthers is ultimately avoided. Of the 11 male-sterile, female-fertile mutants identified in soybean, eight (ms2,ms3,ms5,ms7,ms8,ms9,msMOS,and msp) have been genetically mapped to soybean chromosomes (Jin et al 1998;Cervantes-Martinez et al 2007, 2009Frasch et al 2011;Ott et al 2013;J. Gosh, M.K.…”

“…The highest selfed-seed set was seen when the change in day and night temperature was approximately 6°C. The ms8 gene was located on chromosome 7 (molecular linkage group M) flanked by Sat_389 and the telomere (Frasch et al 2011). The corresponding region was physically 160 Kb and contained 13 genes, of which, three were known to play a role in cell division.…”

Male sterility in soybean: Occurrence, molecular basis and utilization

“…The ms2 gene positioned on LG “O” between markers Sat_190 and Scaa001 with a distance of 6.9 and 9.0 cM, respectively (Cervantes‐Martinez et al, ). Moreover, ms5 , ms7 , ms8 and ms9 were mapped to LG “B1, K, M and N”, respectively (Cervantes‐Martinez et al, ; Frasch, Weigand, Perez, Palmer, & Sandhu, ; Ott et al, ; Yang et al, ), and ms6 gene showed linkage with the flower colour locus w1 (Palmer, Holland, & Lewers, ; Skorupska & Palmer, ). The MFMF gene from male‐sterile and female‐sterile soybean mutant was mapped to LG “D1a”, close to the marker, Satt531 with 9.4 cM distance from the gene (Baumbach et al, ).…”