Molecular Magnetic Resonance Imaging Approaches Used to Aid in the Understanding of Angiogenesis <i>In Vivo</i>: Implications for Tissue Engineering

Towner, Rheal A.; Smith, Nataliya; Asano, Yasuko; He, Ting; Doblas, Sabrina; Saunders, Debra; Silasi‐Mansat, Robert; Lupu, Florea; Seeney, Charles E.

doi:10.1089/ten.tea.2009.0233

Cited by 32 publications

(26 citation statements)

References 25 publications

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“…20 As shown in Figure 4, Prussian blue staining was seen in cancer sections from mice in the anti-VEGF-NP group on days 1, 2, and 9. The anti-VEGF-NPs were clearly retained in endothelial cells, in the area around blood vessels, and in tumor cells.…”

Section: Resultsmentioning

confidence: 81%

“…29 The antibody component attached to the NPs, which allows for the differentiation between specific binding to specific molecular targets, and which allows for nonspecific binding in a 2-4 hour timeframe, may also allow monitoring in a 12-48 hour window, as the antibody may stay bound for a couple of days. 20 Our previous study demonstrated that anti-VEGF-NPs can be used to explore the relationship between tumor burden and the serum VEGF concentration in rats with hepatocellular carcinoma in an immunomagnetic reduction assay. 16 In the present study, anti-VEGF-NPs were present in the colon tumor in mice and showed a high signal-to-noise ratio and selective accumulation in the tumor compared to NPs during in vivo optical imaging.…”

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confidence: 99%

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In vivo tumor targeting and imaging with anti-vascular endothelial growth factor antibody-conjugated dextran-coated iron oxide nanoparticles

Hsieh

Liang

Chieh

et al. 2012

IJN

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Background Active targeting by specific antibodies combined with nanoparticles is a promising technology for cancer imaging and detection by magnetic resonance imaging (MRI). The aim of the present study is to investigate whether the systemic delivery of antivascular endothelial growth factor antibodies conjugating to the surface of functionalized supermagnetic iron oxide nanoparticles (anti-VEGF-NPs) led to target-specific accumulation in the tumor. Methods The VEGF expression in human colon cancer and in Balb/c mice bearing colon cancers was examined by immunohistochemistry. The distribution of these anti-VEGF-NPs particles or NPs particles were evaluated by MRI at days 1, 2, or 9 after the injection into the jugular vein of Balb/c mice bearing colon cancers. Tumor and normal tissues (liver, spleen, lung, and kidney) were collected and were examined by Prussian blue staining to determine the presence and distribution of NPs in the tissue sections. Results VEGF is highly expressed in human and mouse colon cancer tissues. MRI showed significant changes in the T* 2 signal and T 2 relaxation in the anti-VEGF-NP- injected-mice, but not in mice injected with NP alone. Examination of paraffin sections of tumor tissues stained for the iron constituent of the NPs with Prussian blue revealed a strong blue reaction in the tumors of anti-VEGF-NP-treated mice, but only a weak reaction in mice injected with NPs. In both groups, at all time points, Prussian blue-stained liver and spleen sections showed only light staining, while stained cells were rarely detected in kidney and lung sections. Transmission electron microscopy showed that many more electron-dense particles were present in endothelial cells, tumor cells, and extracellular matrix in tumor tissues in mice injected with anti-VEGF-NPs than in NP-injected mice. Conclusion These results demonstrated in vivo tumor targeting and efficient accumulation of anti-VEGF-NPs in tumor tissues after systemic delivery in a colon cancer model, showing that anti-VEGF-NPs have potential for use as a molecular-targeted tumor imaging agent in vivo.

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Section: Resultsmentioning

confidence: 81%

mentioning

confidence: 99%

In vivo tumor targeting and imaging with anti-vascular endothelial growth factor antibody-conjugated dextran-coated iron oxide nanoparticles

Hsieh

Liang

Chieh

et al. 2012

IJN

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“…(29) that involves the differentiation between the earlier "positive-zero-crossing" phase and the current "positive-zero-crossing" phase will modify the proportionality of the heat dissipation to the first term in Eq. (29). Collectively, the time-varying heat dissipation upon MNPs due to frequency-chirped AMF mediation will give rise to a thermoacoustic wave.…”

Section: Iic Frequency-domain Magneto Thermoacoustics From Mnps Expmentioning

confidence: 99%

Magnetothermoacoustics from magnetic nanoparticles by short bursting or frequency chirped alternating magnetic field: A theoretical feasibility analysis

et al. 2013

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Purpose:To propose an alternative method of thermoacoustic wave generation based on heating of magnetic nanoparticles (MNPs) using alternating magnetic field (AMF). Methods: The feasibility of thermoacoustic wave generation from MNPs by applying a short-burst of AMF or a frequency-modulated AMF is theoretically analyzed. As the relaxation of MNPs is strongly dependent upon the amplitude and frequency of AMF, either an amplitude modulated, fixed frequency AMF (termed time-domain AMF) or a frequency modulated, constant amplitude AMF (termed frequency-domain AMF) will result in time-varying heat dissipation from MNPs, which has the potential to generate thermoacoustic waves. Following Rosensweig's model of specific power loss of MNPs in a steady-state AMF, the time-resolved heat dissipations of MNPs of superparamagnetic size when exposed to a short bursting of AMF and/or to a linearly frequency chirped AMF are derived, and the resulted acoustic propagation is presented. Based on experimentally measured temperaturerise characteristics of a superparamagnetic iron-oxide nanoparticle (SPION) matrix in a steady-state AMF of various frequencies, the heat dissipations of the SPION under time-domain and frequencydomain AMF configurations that could have practical utility for thermoacoustic wave generation are estimated. Results:The initial rates of the temperature-rise of the SPION matrix were measured at an ironweight concentration of 0.8 mg/ml and an AMF frequency of 88.8 kHz to 1.105 MHz. The measured initial rates of temperature-rise were modeled by Rosensweig's theory, and projected to 10 MHz AMF frequency, at which a 1 μs bursting corresponding to a 1.55 mm axial resolution of acoustic detection could contain 10 complete cycles of AMF oscillation and the power dissipation is approximately 84 times of that at 1 MHz. Exposing the SPION matrix to a 1 μs bursting of AMF at 10 MHz frequency and 100 Oe field intensity would produce a volumetric heat dissipation of 7.7 μJ/cm 3 over the microsecond duration of the AMF burst. If the SPION matrix is exposed to a 1 ms long AMF train at 100 Oe field intensity that chirps linearly from 1 to 10 MHz, the volumetric heat dissipation produced over each 2π phase change of the AMF oscillation is estimated to increase from 0.15 to 1.1 μJ/cm 3 within the millisecond duration of the chirping of AMF. Conclusions: The heat dissipations upon SPION (∼1 mg/ml iron-weight concentration) by a 1 μs bursting of 100 Oe AMF at 10 MHz and a 1 ms train of 100 Oe AMF that chirps linearly from 1 to 10 MHz were estimated to determine the potential of thermal-acoustic wave generation. Although thermoacoustic wave generation from MNPs by time-or frequency-domain AMF applications is predicted, the experimental generation of such a wave remains challenging. © 2013 American Association of Physicists in Medicine. [http://dx

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“…9 Various ligands with specific targeting abilities have offered unique opportunities for enhanced accumulation of IONPs and specificity to glioma, eg, RGD peptide, 39 chlorotoxin, 40 epidermal growth factor, 41 heat shock protein 70, 42 lactoferrin, 43,44 transferrin 45 and antibodies (anti-insulinlike-growth-factor binding protein 7 single domain antibody, 46 anti-vascular endothelial growth factor receptor 2 47 ). The targeted IONPs could reduce T2 and T2* relaxation times, providing tumor-specific contrast on susceptibility-weighted images 44,48 or T2*-weighted images 41,42 for improved tumor delineation.…”

Section: Preparation and Characterization Of Npsmentioning

confidence: 99%

Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging

Liu¹,

Chen²,

Xue³

et al. 2016

IJN

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The highly infiltrative and invasive nature of glioma cells often leads to blurred tumor margins, resulting in incomplete tumor resection and tumor recurrence. Accurate detection and precise delineation of glioma help in preoperative delineation, surgical planning and survival prediction. In this study, recombinant epidermal growth factor-like domain-1, derived from human coagulation factor VII, was conjugated to iron oxide nanoparticles (IONPs) for targeted glioma magnetic resonance (MR) imaging. The synthesized EGF1-EGFP-IONPs exhibited excellent targeting ability toward tissue factor (TF)-positive U87MG cells and human umbilical vein endothelial cells in vitro, and demonstrated persistent and efficient MR contrast enhancement up to 12 h for preclinical glioma models with high targeting specificity in vivo. They hold great potential for clinical translation and developing targeted theranostics against brain glioma.

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Molecular Magnetic Resonance Imaging Approaches Used to Aid in the Understanding of Angiogenesis In Vivo: Implications for Tissue Engineering

Cited by 32 publications

References 25 publications

In vivo tumor targeting and imaging with anti-vascular endothelial growth factor antibody-conjugated dextran-coated iron oxide nanoparticles

In vivo tumor targeting and imaging with anti-vascular endothelial growth factor antibody-conjugated dextran-coated iron oxide nanoparticles

Magnetothermoacoustics from magnetic nanoparticles by short bursting or frequency chirped alternating magnetic field: A theoretical feasibility analysis

Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging

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