Molecular Ionization-Desorption Analysis Source (MIDAS) for Mass Spectrometry: Thin-Layer Chromatography

Winter, Gregory T.; Wilhide, Joshua A.; LaCourse, William R.

doi:10.1007/s13361-015-1289-5

Cited by 14 publications

(15 citation statements)

References 33 publications

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“…Thus, the isolated ions at m / z 130 were used for quantitation. In general, a 2 to 4 orders magnitude improvement in the LODs was obtained, which is better than any of the LODs reported to date on plasma-based ambient desorption ionization MS from TLC plates, as discussed in the previous sections. ,,,, In addition, the LODs in Table are also comparable to the performance reported for amino acids with MALDI-TOF-MS . It needs to be pointed out that in that study standard solutions were directly pipetted on the MALDI sample plate, not on TLC plates where the desorption is affected by the strong interaction between the analyte and the TLC matrix, and using internal standards for quantitation.…”

Section: Resultsmentioning

confidence: 49%

“…For example, a detection limit of 370 ng for acetaminophen was reported by APCI-MS, in which the desorption was accomplished by a heated metal probe at 350 °C placed near-by or just touching the NP-TLC plate surface, whereas, in the present study, the LOD of 1.2 pg/mm 2 (∼11 pg per sample spot) for acetaminophen was achieved by LD-LTP, which is more than 4 orders of magnitudes better (Table ). In addition, the molecular ionization-desorption analysis source (MIDAS), with discharge nitrogen gas heated up to 180 °C, implemented for direct sampling from silica gel TLC plates showed a linear calibration curve from 50 to 1000 ng of caffeine per spot, no LODs reported . Another recent published study by Garcia-Rojas et al reported LODs of caffeine in 20 ng/mm 2 on polyethylene-supported TLC plates by LTP-MS with laser desorption .…”

Section: Resultsmentioning

confidence: 99%

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Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Gong

Zhang

Embile

et al. 2020

J. Am. Soc. Mass Spectrom.

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Thin-layer chromatography (TLC) is a widespread technique because it allows fast, simple, and inexpensive analyte separations. In addition, direct analysis of the compounds separated on TLC plates via mass spectrometry (MS) has been shown to provide high sensitivity and selectivity while avoiding time-consuming sample extraction protocols. Here, direct desorption low-temperature plasma-mass spectrometry (LTP-MS) as well as diode laser assisted desorption (LD) LTP-MS are studied for direct spatially resolved analysis of compounds from TLC plates. Qualitative and quantitative characterization of amino acids, pharmaceuticals, and structural isomers were performed. The nature of the TLC plate stationary phase was found to have a significant influence, together with the analyte's characteristics, on the desorption efficiency. Tandem MS is shown to greatly improve the limits of detection (LODs). Direct desorption LTP-MS, without external thermal assisted desorption, demonstrates its best performance with cellulose TLC plates (LODs, 0.01 ng/mm 2 to 2.55 ng/mm 2 ) and restricted performance with normal-phase (NP) TLC plates (several analytes without observable signal). LD LTP-MS, with systematic optimization of irradiance and focal point diameter, is shown to overcome the direct-desorption limitations and reach significantly improved LODs with NP TLC plates (up to ×1000 better). In addition, a wide-ranging characterization of amino acid analytical figures of merit with LD LTP-MS shows that LODs from 84 pg/mm 2 down to 0.3 pg/mm 2 are achieved on NP TLC plates.

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Section: Resultsmentioning

confidence: 49%

Section: Resultsmentioning

confidence: 99%

Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Gong

Zhang

Embile

et al. 2020

J. Am. Soc. Mass Spectrom.

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“…One approach to reduce ion suppression and matrix effects is to combine ADI sources with planar chromatography techniques such as thin-layer chromatography (TLC) or high-performance thin-layer chromatography (HPTLC). For example, the benefit of coupling TLC to DESI-MS [ 15 – 17 ], DART-MS [ 18 – 20 ], low-temperature plasma (LTP) [ 21 ], and molecular ionization-desorption analysis source (MIDAS) [ 22 , 23 ] has been demonstrated in several studies. Other plasma-based ADI sources such as the flowing atmospheric-pressure afterglow (FAPA) source were used in TLC-ADI-MS studies as well [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative detection of caffeine in beverages using flowing atmospheric-pressure afterglow (FAPA) ionization high-resolution mass spectrometry imaging and performance evaluation of different thin-layer chromatography plates as sample substrates

2022

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Ambient desorption/ionization mass spectrometry (ADI-MS) is widely used as a rapid screening tool of samples in their native state without sample preparation. While analysis times are much less than 1 min per sample, one challenge of ADI-MS is the possibility to perform quantitative analysis of analytes in complex matrices. Typically, the goal is to probe a variety of different analytes in a complex matrix from a solid, liquid, or otherwise uncharacterized surface in the open air in front of the MS inlet. In this study, it is demonstrated that a carefully selected surface for analyte spot sampling and co-deposited isotopically labeled standards both significantly improve the capabilities of flowing atmospheric-pressure afterglow (FAPA) high-resolution (HR) MS for direct quantitative analysis. Specifically, a systematic study of different surfaces (glass, steel mesh, high-performance thin-layer chromatography (HPTLC) stationary phases including silica, reversed-phase (RP)-modified silica, and cyano (CN)-modified silica) and their suitability for spot sampling with FAPA-MS was performed. A set of different caffeine-containing standards and beverages (Red Bull, Coca-Cola, coffee, and black tea) was deposited on the surfaces and direct FAPA-HR-MS analysis of caffeine was performed using internal calibration with co-deposited 13C3-caffeine. For TLC surfaces, it was demonstrated that quantitative results could be achieved with the matrix and concomitants present and that a preceding chromatographic separation was not mandatory for this application. In addition, the use of a CN-HPTLC surface resulted in a significantly more intense caffeine signal in the beverage samples compared to the other surfaces studied, with the highest increase compared to the silica (200-fold higher) and the lowest increase compared to the steel mesh (30-fold higher). The utilization of TLC-based surfaces as sample carriers is considered an attractive tool in the ADI-MS toolbox for fast and efficient mass spectrometric investigations of complex samples without time-consuming sample preparation. Graphical Abstract

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“…A filter paper containing algal agent was placed in the solid culture medium, and the ability of algal growth inhibition was determined by measuring the diameter of the blank region of the algae cell death in the solid medium. After comparing the inhibition zone diameters obtained using different extracts, the extract with the optimal inhibition on algal growth was selected for subsequent thin layer chromatography (TLC) [30,31] and column chromatography experiments for separation and purification.…”

Section: Extraction and Separation Of Compounds Present In E Dulcis mentioning

confidence: 99%

Allelochemicals Extracted from Eleocharis dulcis and their Inhibitory Effects on Microcystis aeruginosa

Wen¹,

Shu²,

Chen³

et al. 2017

J Chem Eng Process Technol

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Allelochemicals are considered to be effective substances for controlling the outbreak of harmful cyanobacteria. In this study, Eleocharis dulcis peel fractions with an inhibitory effect on Microcystis aeruginosa were isolated by extraction and purification. The extracted organic compounds were identified by gas chromatography-mass spectrometry (GC-MS), and the allelopathic effect and mechanism underlying the inhibition of M. aeruginosa were explored. The results demonstrated that the exogenous compound, eriodictyol, corresponding to one of the detected flavonoids (C H 12 O 6) could inhibit the growth of M. aeruginosa, indicating that the flavonoid C 15 H 12 O 6 is one of the allelochemicals contained in E. dulcis. Levels of chlorophyll a (Chl-a), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and reduced glutathione (GSH) of cell health in M. aeruginosa were analysed. Our results show that the damage to the antioxidant enzymes of algal cells caused by eriodictyol could be a main contributing factor to the decrease in the number of algal cells and even cell death.

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Molecular Ionization-Desorption Analysis Source (MIDAS) for Mass Spectrometry: Thin-Layer Chromatography

Cited by 14 publications

References 33 publications

Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Quantitative detection of caffeine in beverages using flowing atmospheric-pressure afterglow (FAPA) ionization high-resolution mass spectrometry imaging and performance evaluation of different thin-layer chromatography plates as sample substrates

Allelochemicals Extracted from Eleocharis dulcis and their Inhibitory Effects on Microcystis aeruginosa

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