Molecular interactions of tannic acid and matrix metalloproteinases 2 and 9

Chiang, Ya-Tang; Xiao, Yu-Bai; Hsu, Shan‐hui; Chang, Shu‐Wei; Chou, C.K.

doi:10.1016/j.csbj.2023.04.011

Cited by 5 publications

(1 citation statement)

References 50 publications

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“…Studies have shown that naturally occurring polyphenolic compounds can interact with collagen fibers through phenolic hydroxyl and galloyl groups to form covalent, ionic, and hydrogen bonds. They can also alter the spatial conformation of collagen, form cross-links with endogenous MMPs or CCs, obstruct enzyme recognition sites, enhance the mechanical properties of collagen, lessen its sensitivity to enzymes, and locally create a hydrophobic microenvironment that lowers water absorption and swelling ratio ( Liu et al, 2015 ; Reis et al, 2021 ; Chiang et al, 2023 ). As mentioned above, recent studies have highlighted the potential of natural polyphenols in dentin biomodification, with PA being particularly noted for its efficacy in this domain ( Wang B. et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Theaflavin −3,3'-digallate/ethanol: a novel cross-linker for stabilizing dentin collagen

Chen,

Wei,

Liang

et al. 2024

Front. Bioeng. Biotechnol.

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ObjectivesTo study the ability of theaflavin-3,3’-digallate (TF3)/ethanol solution to crosslink demineralized dentin collagen, resist collagenase digestion, and explore the potential mechanism.MethodsFully demineralized dentin blocks were prepared using human third molars that were caries-free. Then, these blocks were randomly allocated into 14 separate groups (n = 6), namely, control, ethanol, 5% glutaraldehyde (GA), 12.5, 25, 50, and 100 mg/ml TF3/ethanol solution groups. Each group was further divided into two subgroups based on crosslinking time: 30 and 60 s. The efficacy and mechanism of TF3’s interaction with dentin type I collagen were predicted through molecular docking. The cross-linking, anti-enzymatic degradation, and biomechanical properties were studied by weight loss, hydroxyproline release, scanning/transmission electron microscopy (SEM/TEM), in situ zymography, surface hardness, thermogravimetric analysis, and swelling ratio. Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy were utilized to explore its mechanisms. Statistical analysis was performed using one and two-way analysis of variance and Tukey’s test.ResultsTF3/ethanol solution could effectively crosslink demineralized dentin collagen and improve its resistance to collagenase digestion and biomechanical properties (p < 0.05), showing concentration and time dependence. The effect of 25 and 50 mg/ml TF3/ethanol solution was similar to that of 5% GA, whereas the 100 mg/mL TF3/ethanol solution exhibited better performance (p < 0.05). TF3 and dentin type I collagen are mainly cross-linked by hydrogen bonds, and there may be covalent and hydrophobic interactions.ConclusionTF3 has the capability to efficiently cross-link demineralized dentin collagen, enhancing its resistance to collagenase enzymatic hydrolysis and biomechanical properties within clinically acceptable timeframes (30 s/60 s). Additionally, it exhibits promise in enhancing the longevity of dentin adhesion.

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Section: Introductionmentioning

confidence: 99%

Theaflavin −3,3'-digallate/ethanol: a novel cross-linker for stabilizing dentin collagen

Chen,

Wei,

Liang

et al. 2024

Front. Bioeng. Biotechnol.

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Approaches to Control and Monitor Protease Levels in Chronic Wounds

Kolahreez,

Ghasemi‐Mobarakeh,

Liebner

et al. 2024

Advanced Therapeutics

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Some wounds do not follow the typical healing trajectory and show very little, if any, progress. They dreadfully threaten the patients' quality of life and may even lead to limb amputation and death. Several interrelated systemic and local factors may cause a delay in wound healing. The microenvironment of these wounds could be different from wounds that heal spontaneously or with standard treatment. As discussed in the present work, proteases’ levels or activity could be elevated in some cases of chronic ulcers; infection could aggravate the situation. A detailed overview of the proteases’ effect on wound healing is presented in this paper. Assuming untimely or irregularly excessive proteolytic activity plays a pathological role, some researchers targeted attenuation of protease level as a strategy to promote healing. This could be done via different approaches, including physical removal, inhibiting their activity, and controlling their expression directly through gene silencing or indirectly, for instance, by resolving infection. Apart from targeting protease modulation as a therapeutic strategy, many studies have examined the protease state to gain insights into the underlying mechanisms by which a remedy, dressing, or specific therapy has affected the healing process. This article aims to provide an overview of the studies in these two categories. Of course, various proteases are involved in the healing process, but in each study, usually, only one or some of them were investigated; matrix metalloproteinase‐2 (MMP‐2), MMP‐9, and human neutrophil elastase (HNE) are among them. Furthermore, in some cases, cumulative proteolytic activity has been evaluated in terms of gelatinolytic, collagenolytic, or caseinolytic activity, which is also absolutely beneficial. Some research groups have considered the potential of proteases as a diagnostic or prognostic biomarker and have tried to develop some sensors or indicators; this topic has also been covered in the present work.This article is protected by copyright. All rights reserved

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In vitro and in silico screening and identification of potential bioactive anthraquinones of Morinda lucida benth against pathogenic bacterial target proteins

Adekunle,

Adeleke,

Odugbemi

et al. 2024

Discov Appl Sci

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Background The use of plant extracts as antibiotics is gaining more attention because bacteria are one of the biggest threats to global health and the resistance of bacteria to antibiotics in humans and animals is increasing. Antibacterial susceptibility is used to determine which specific antibiotics a particular bacterium is sensitive to. This research is focused on the phytochemical, in vitro antibacterial susceptibility, and in silico analysis of Morinda lucida root extracts against gram-negative Pseudomonas aeruginosa and gram-positive Staphylococcus aureus. The root of M. lucida was extracted separately with ethanol, chloroform, and ethyl acetate. Results Crude extracts of M. lucida had active antibacterial activity and were a promising natural antibiotic when compared to gentamicin. The in vitro results showed that the extracts of M. lucida had good susceptibility properties against the two drug-resistant bacteria while the in silico showed that 2-hydroxy-1-methoxy anthraquinone is predicted to have a better susceptibility with S. aureus while alizarin has better susceptibility properties against P. aeruginosa. Finally, the MD simulation studies of Alizarin and 9,10-Anthracenedione 2-hydroxy-1- methoxy- define the stability of protein–ligand complexes within a 50 ns time scale.

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Molecular interactions of tannic acid and matrix metalloproteinases 2 and 9

Cited by 5 publications

References 50 publications

Theaflavin −3,3'-digallate/ethanol: a novel cross-linker for stabilizing dentin collagen

Theaflavin −3,3'-digallate/ethanol: a novel cross-linker for stabilizing dentin collagen

Approaches to Control and Monitor Protease Levels in Chronic Wounds

In vitro and in silico screening and identification of potential bioactive anthraquinones of Morinda lucida benth against pathogenic bacterial target proteins

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