Molecular interactions of natural and synthetic steroids in female hamsters’ flank organs

Effect of Carbamates on mRNA Encoding Lipid Enzymes in Hamster Flank Organs

“…Treatments with T or P showed a higher expression as indicated by the results in Table . These data were similar to those previously reported by us . Nevertheless, P + 7 – 10 and T + 7 – 10 decreased significantly the mRNA expression encoding for GPAT (Table ).…”

“…However, 8 and 9 decreased the size of the pigmented spots of gonadectomized females treated with T. Since both steroids inhibited the activity of 5α‐reductase, DHT was not formed and as result of this, the diameter size was not increased. Similar results had been previously reported using different inhibitors of 5α‐reductase and was also previously found by our group using 7 and 10 carbamate derivatives of progesterone 5 .…”

“…The overall data taken together could be explained on the ground that 5α‐dihydrotestosterone (5α‐DHT) and 5α‐DHP are transcriptional factors that codified for GPAT, HMG‐CoA‐S and HMG‐CoA‐R, PI‐S, and SQ‐S . However, P could also be a transcription factor for the expression of SQ‐S.…”

“…Specific GPAT, HMG‐CoA‐S, HMG‐CoA‐R, PI‐S, and SQ‐S as well as β‐actin mRNAs were examined by one‐step reverse transcription (Qiagen OneStep RT‐PCR Kit, Valencia, CA, USA) following the manufacturer's instructions .…”

“…The higher lipids synthesized by this pilosebaceous unit were cholesterol esters . Progesterone 5 also increased mRNA expression for lipid enzymes: glycerol 3‐phosphate acyl transferase (GPAT), β‐hydroxy‐β‐methylglutaryl‐CoA synthase (HMG‐CoA‐S), β‐hydroxy‐β‐methylglutaryl‐CoA reductase (HMG‐CoA‐R), phosphatidylinositol synthase (PI‐S), and squalene‐synthase (SQ‐S) in female hamster's flank organs .…”