Molecular Interactions of GBF1 with HY5 and HYH Proteins during Light-mediated Seedling Development in Arabidopsis thaliana

Singh, Aparna; Ram, Hasthi; Abbas, Nazia; Chattopadhyay, Sudip

doi:10.1074/jbc.m111.333906

Cited by 88 publications

(122 citation statements)

References 45 publications

(56 reference statements)

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“…The expression of HY5 in the cam7 hy5 double mutant also supports this notion ( Figure 8D). HY5 is known to interact and form heterodimers with at least two other bZIP proteins, GBF1/ ZBF2 and HYH, during Arabidopsis seedling development (Holm et al, 2002;Singh et al, 2012). Therefore, higher levels of HY5 might also interact or form heterodimers with other regulatory proteins in the regulation of HY5 expression.…”

Section: Discussionmentioning

confidence: 99%

“…For this experiment, the full-length CAM7 coding sequence was fused to the N terminus of YFP in the pUC-SPYNE vector and the full-length HY5 coding sequence was fused to the C terminus of YFP in the pUC-SPYCE vector (Singh et al, 2012). Whereas empty vectors did not produce any YFP fluorescence (Figure 7E), the interaction of CAM7 and HY5 produced strong YFP fluorescence in the nucleus ( Figure 7F).…”

Section: Cam7 Colocalizes and Physically Interacts With Hy5 In Onion mentioning

confidence: 99%

“…It has been reported that the massive change in gene expression is mediated by several transcriptional cascades (Tepperman et al, 2001). The transcriptional network plays a key role in mediating the light signal through the coordinated activation and repression of downstream genes (Mallappa et al, 2008;Singh et al, 2012). HY5 has been genetically defined as a positive regulator based on its partially etiolated phenotype of light-grown mutant seedlings (Koornneef et al, 1980;Ang and Deng, 1994;Oyama et al, 1997;Pepper and Chory, 1997).…”

Section: Introductionmentioning

confidence: 99%

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ArabidopsisCAM7 and HY5 Physically Interact and Directly Bind to theHY5Promoter to Regulate Its Expression and Thereby Promote Photomorphogenesis

et al. 2014

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Arabidopsis thaliana CALMODULIN7 (CAM7), a unique member of the calmodulin gene family, plays a crucial role as a transcriptional regulator in seedling development. The elongated HYPOCOTYL5 (HY5) bZIP protein, an integrator of multiple signaling pathways, also plays an important role in photomorphogenic growth and light-regulated gene expression. CAM7 acts synergistically with HY5 to promote photomorphogenesis at various wavelengths of light. Although the genetic relationships between CAM7 and HY5 in light-mediated seedling development have been demonstrated, the molecular connectivity between CAM7 and HY5 is unknown. Furthermore, whereas HY5-mediated gene regulation has been fairly well investigated, the transcriptional regulation of HY5 is largely unknown. Here, we report that HY5 expression is regulated by HY5 and CAM7 at various wavelengths of light and also at various stages of development. In vitro and in vivo DNA-protein interaction studies suggest that HY5 and CAM7 bind to closely located T/G-and E-box cis-acting elements present in the HY5 promoter, respectively. Furthermore, CAM7 and HY5 physically interact and regulate the expression of HY5 in a concerted manner. Taken together, these results demonstrate that CAM7 and HY5 directly interact with the HY5 promoter to mediate the transcriptional activity of HY5 during Arabidopsis seedling development.

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Section: Discussionmentioning

confidence: 99%

Section: Cam7 Colocalizes and Physically Interacts With Hy5 In Onion mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ArabidopsisCAM7 and HY5 Physically Interact and Directly Bind to theHY5Promoter to Regulate Its Expression and Thereby Promote Photomorphogenesis

et al. 2014

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“…To our surprise, OsbZIP23 could directly bind to the promoters of eight other OsPP2Cs under drought stress conditions (Supplemental Table S2). However, only OsPP2C49 and OsPP2C51 were differentially regulated by OsbZIP23 in the RNA-seq results (Supplemental Table S2), which indicates that the regulation of different clade A PP2Cs may need different transcription factors such as NACs and MYCs to coordinate with bZIP transcription factors for the differential regulation (Singh et al, 2012;Xu et al, 2013;Maurya et al, 2015).…”

Section: Osbzip23 Feedback Regulates Aba Signaling By Directly Targetmentioning

confidence: 97%

Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes

et al. 2016

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The OsbZIP23 transcription factor has been characterized for its essential role in drought resistance in rice (Oryza sativa), but the mechanism is unknown. In this study, we first investigated the transcriptional activation of OsbZIP23. A homolog of SnRK2 protein kinase (SAPK2) was found to interact with and phosphorylate OsbZIP23 for its transcriptional activation. SAPK2 also interacted with OsPP2C49, an ABI1 homolog, which deactivated the SAPK2 to inhibit the transcriptional activation activity of OsbZIP23. Next, we performed genome-wide identification of OsbZIP23 targets by immunoprecipitation sequencing and RNA sequencing analyses in the OsbZIP23-overexpression, osbzip23 mutant, and wild-type rice under normal and drought stress conditions. OsbZIP23 directly regulates a large number of reported genes that function in stress response, hormone signaling, and developmental processes. Among these targets, we found that OsbZIP23 could positively regulate OsPP2C49, and overexpression of OsPP2C49 in rice resulted in significantly decreased sensitivity of the abscisic acid (ABA) response and rapid dehydration. Moreover, OsNCED4 (9-cis-epoxycarotenoid dioxygenase4), a key gene in ABA biosynthesis, was also positively regulated by OsbZIP23. Together, our results suggest that OsbZIP23 acts as a central regulator in ABA signaling and biosynthesis, and drought resistance in rice.

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“…FHY3 binds to an FHY3 binding site and HY5 to an ACGT-containing element in the COP1 promoter, both of which seem to be required for the UV-B responsiveness of COP1 . Combinatorial regulation exerted by the interaction of other transcription factors with HY5/HYH is widely present, with negative or positive regulatory effects (Shin et al, 2007;Andronis et al, 2008;Holtan et al, 2011;Huang et al, 2012;Jiang et al, 2012;Singh et al, 2012;Gangappa et al, 2013aGangappa et al, , 2013bJing et al, 2013;Abbas et al, 2014). Moreover, HY5 interacts with the chromatin-remodeling factor PICKLE (PKL), recruiting PKL to promoters of target genes repressing the H3K27me3-repressive histone mark (Jing et al, 2013).…”

Section: Regulation Of the Hy5 Association With Chromatinmentioning

confidence: 99%

UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

et al. 2014

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In plants subjected to UV-B radiation, responses are activated that minimize damage caused by UV-B. The bZIP transcription factor ELONGATED HYPOCOTYL5 (HY5) acts downstream of the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) and promotes UV-B-induced photomorphogenesis and acclimation. Expression of HY5 is induced by UV-B; however, the transcription factor(s) that regulate HY5 transcription in response to UV-B and the impact of UV-B on the association of HY5 with its target promoters are currently unclear. Here, we show that HY5 binding to the promoters of UV-B-responsive genes is enhanced by UV-B in a UVR8-dependent manner in Arabidopsis thaliana. In agreement, overexpression of REPRESSOR OF UV-B PHOTOMORPHOGENESIS2, a negative regulator of UVR8 function, blocks UV-B-responsive HY5 enrichment at target promoters. Moreover, we have identified a T/G-box in the HY5 promoter that is required for its UV-B responsiveness. We show that HY5 and its homolog HYH bind to the T/G HY5 -box cis-acting element and that they act redundantly in the induction of HY5 expression upon UV-B exposure. Therefore, HY5 is enriched at target promoters in response to UV-B in a UVR8 photoreceptor-dependent manner, and HY5 and HYH interact directly with a T/G-box cis-acting element of the HY5 promoter, mediating the transcriptional activation of HY5 in response to UV-B.

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Molecular Interactions of GBF1 with HY5 and HYH Proteins during Light-mediated Seedling Development in Arabidopsis thaliana

Cited by 88 publications

References 45 publications

ArabidopsisCAM7 and HY5 Physically Interact and Directly Bind to theHY5Promoter to Regulate Its Expression and Thereby Promote Photomorphogenesis

ArabidopsisCAM7 and HY5 Physically Interact and Directly Bind to theHY5Promoter to Regulate Its Expression and Thereby Promote Photomorphogenesis

Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes

UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

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