Molecular interaction of charcoal rot pathogenesis in soybean: a complex interaction

Deshmukh, Reena; Tiwari, Sharad

doi:10.1007/s00299-021-02747-9

Cited by 9 publications

(4 citation statements)

References 111 publications

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“…The NAC transcription factor family, known for its hypersensitivity to pathogen invasion, may activate chitinase to confer resistance. Moreover, the WRKY, NBs-LRR, and flavonoid pathways have been implicated in charcoal rot resistance [11, 23]. The scientist [11] investigated the ability of soybean to resist charcoal rot and reported the involvement of genes related to WRKY, NBs-LRR, and flavonoid pathways, which aligns with our findings.…”

Section: Discussionsupporting

confidence: 88%

“…Moreover, the WRKY, NBs-LRR, and flavonoid pathways have been implicated in charcoal rot resistance [11, 23]. The scientist [11] investigated the ability of soybean to resist charcoal rot and reported the involvement of genes related to WRKY, NBs-LRR, and flavonoid pathways, which aligns with our findings. The WRKY protein, encoded by 174 genes and responsive to salicylic acid, contains zinc finger motifs and a conserved sequence [24], similar to the findings in our study where zinc finger proteins were significantly enhanced in both susceptible and resistant genotypes treated with silicon, along with the ribosomal-like protein L16.…”

Section: Discussionsupporting

confidence: 88%

“…Numerous studies support the use of the transcriptomic (RNA-Seq) approach to pinpoint the molecular evidences of disease resistance [11] & [12]. The rapidly expanding field of transcriptomic has made it feasible to gain a thorough grasp of the intricate connections between plants and their biotic stress.…”

Section: Introductionmentioning

confidence: 99%

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Unravelling Silicon’s Transcriptomic Armor in Soybean againstMacrophomina phaseolinacausing Charcoal Rot Disease

Jadhav,

Magar,

Sharma

et al. 2023

Preprint

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TheGlycine maxL. has been affected by more than 100 diseases, includingMacrophomina phaseolinaproducing charcoal rot disease, which reduces production by 70%. In this investigation, RNA-Seq analysis is used for the first time to explore role of silicon in preventing soybean charcoal rot. The study explores the molecular mechanism underlying soybeans’ resilience to charcoal rot when treated with potassium silicon. It was meticulously investigated howMacrophomina phaseolinaentered the roots. The SEM, which showed a strong link between potassium silicate accumulation and disease resistance. Further investigation indicates that a potassium silicate concentration of 1.7mM lowers disease incidence. Using Illumina HiSeq NGS data, we present a transcriptome analysis revealing genes associated with charcoal rot resistance, highlighting 3,106 genes with distinct expression patterns. The strong enrichment of pathways including “Biosynthesis of ansamycins” and “Flavone and flavonol biosynthesis,” which contribute to resistance against charcoal rot, is highlighted by KEGG enrichment analysis. The ERF transcription factor and NB leucine-rich repeats stands out among the differentially expressed genes as being particularly connected to resistance. The crucial functions that many other important transcription factors, including as MYB, NAC, and proteins from the FAR1 family, play in enhancing soybeans’ resistance to charcoal rot are also noted. This newly discovered information could help in developing tactics to strengthen soybean’s resistance toMacrophomina phaseolina.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

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Unravelling Silicon’s Transcriptomic Armor in Soybean againstMacrophomina phaseolinacausing Charcoal Rot Disease

Jadhav,

Magar,

Sharma

et al. 2023

Preprint

View full text Add to dashboard Cite

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“…Furthermore, the identi ed DEGs were involved in networks related to DNA replication and iron homeostasis [78]. Charcoal rot caused by Macrophomina phaseolina revealed that WRKY, LysM-RLKs, NBS-LRR, and avonoid pathway-related genes could be targeted in breeding programs to combat this disease [79]. Kim et al [80] revealed the genes involved in the plant defense mechanism against bacterial leaf pustule caused by Xanthomonas axonopodis pv.…”

Section: Rna-seq Analysis For Biotic Stress Resistance In Soybeanmentioning

confidence: 99%

Multi-omics assisted breeding for biotic stress resistance in soybean

et al. 2023

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Biotic stress is a critical factor limiting soybean growth and development. Soybean responses to biotic stresses such as insects, nematodes, and fungal, bacterial, and viral pathogens are governed by complex regulatory and defense mechanisms. Next-generation sequencing has availed research techniques and strategies in genomics and postgenomics. This review summarizes the available information on marker resources, quantitative trait loci, and marker trait associations involved in regulating biotic stress responses in soybean. We discuss the differential expression of related genes and proteins reported in different transcriptomics and proteomics studies and the role of signaling pathways and metabolites reported in metabolomic studies. Recent advances in omics technologies offer opportunities to reshape and improve biotic stress resistance in soybean by altering gene regulation and/or other regulatory networks. We recommend using 'integrated omics' to understand how soybean responds to different biotic stresses. We discuss the potential challenges of integrating multiomics for functional analysis of genes and their regulatory networks and the development of biotic stress-resistant cultivars. This review will help direct soybean breeding programs to develop resistance against different biotic stresses.the rapid identi cation of putative genes/quantitative trait loci (QTLs) associated with biotic stress resistance, the pathways involved, and functional analysis of intermediate or nal proteins and metabolites produced. However, there is a lack of comprehensive information on using omics approaches for biotic stress resistance in soybean. Therefore, we review various omics approaches used in soybean to improve crop performance under different biotic stresses to assist future soybean breeding in developing novel resistant cultivars. Soybean GenomicsIn plant breeding, markers are used to construct genetic linkage maps, perform phylogenetic and evolutionary analyses, select desired alleles, and map genes/QTLs. These activities started with the identi cation of several low-throughput markers, such as RFLP, RAPD, and AFLP. Later, comparatively more abundant, highly polymorphic, and co-dominant markers, known as SSRs, complemented the markers mentioned above in soybean genetics, molecular biology, and breeding research [13]. Large numbers of QTLs associated with different biotic stresses have been identi ed in soybean using these markers (Table 1). In addition, marker-assisted selection (MAS) has sped up the breeding process, particularly for producing disease-and insect pest-resistant cultivars [14].Earlier, a high density genetic map 'Soybean Consensus Map (version 4.0)' was constructed by combining available genetic and physical maps involving SSRs and SNPs [15]. Genomic applications in soybean became more frequent with the availability of the whole genome sequence, one of the rst legumes to be sequenced and published [16]. In addition, sequencing-based genotyping approaches such as genotyping by sequencing (GBS) have be...

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