Molecular insights into a molluscan transferrin homolog identified from disk abalone (Haliotis discus discus) evidencing its detectable role in host antibacterial defense

Herath, H.M.L.P.B.; Elvitigala, Don Anushka Sandaruwan; Godahewa, G.I.; Whang, Ilson; Lee, Jehee

doi:10.1016/j.dci.2015.07.013

Cited by 9 publications

(9 citation statements)

References 59 publications

(16 reference statements)

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“…Transferrins bind to iron and limit its availability by creating a bacteriostatic environment for iron-dependent bacteria. For example, Ab-transferrin was up-regulated upon immune stimulation of Haliotis discus discus with Vibrio parahaemolyticus, lipopolysaccharides and Listeria monocytogenes [48]. Additionally, the transcript coding for a homologue of histone H1 (So-H1) was up-regulated after bacterial challenge.…”

Section: Results-discussionmentioning

confidence: 99%

In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

et al. 2020

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Cuttlefish (Sepia officinalis) haemocytes are potential sources of antimicrobial peptides (AMPs). To study the immune response to Vibrio splendidus and identify new AMPs, an original approach was developed based on a differential transcriptomic study and an in-depth in silico analysis using multiple tools. Two de novo transcriptomes were retrieved from cuttlefish haemocytes following challenge by V. splendidus or not. A first analysis of the annotated transcripts revealed the presence of Toll/NF-κB pathway members, including newly identified factors such as So-TLR-h, So-IKK-h and So-Rel/NF-κB-h. Out of the eight Toll/NF-κB pathway members, seven were found up-regulated following V. splendidus challenge. Besides, immune factors involved in the immune response were also identified and up-regulated. However, no AMP was identified based on annotation or conserved pattern searches. We therefore performed an in-depth in silico analysis of unannotated transcripts based on differential expression and sequence characteristics, using several tools available like PepTraq, a homemade software program. Finally, five AMP candidates were synthesized. Among them, NF19, AV19 and GK28 displayed antibacterial activity against Gram-negative bacteria. Each peptide had a different spectrum of activity, notably against Vibrio species. GK28—the most active peptide—was not haemolytic, whereas NF19 and AV19 were haemolytic at concentrations between 50 and 100 µM, 5 to 10 times higher than their minimum inhibitory concentration.

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Section: Results-discussionmentioning

confidence: 99%

In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

et al. 2020

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“…Tranferrin is a regulatory molecule of the innate immune system in mollusks that employs antimicrobial activity against a wide range of Gram-positive and -negative bacteria [37,38]. In H. discus discus , the in vivo antimicrobial property of transferrin through its Fe 3+ binding ability was characterzied [39]. Although the functions of the cubilin protein in the renal proximal tubule of rodents and mammals are well established, information regarding the gene and its molecular mechanisms remains unclear in aquatic invertebrates.…”

Section: Resultsmentioning

confidence: 99%

Alternative Splicing Profile and Sex-Preferential Gene Expression in the Female and Male Pacific Abalone Haliotis discus hannai

Kim

Rhee

Kim

et al. 2017

Genes

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In order to characterize the female or male transcriptome of the Pacific abalone and further increase genomic resources, we sequenced the mRNA of full-length complementary DNA (cDNA) libraries derived from pooled tissues of female and male Haliotis discus hannai by employing the Iso-Seq protocol of the PacBio RSII platform. We successfully assembled whole full-length cDNA sequences and constructed a transcriptome database that included isoform information. After clustering, a total of 15,110 and 12,145 genes that coded for proteins were identified in female and male abalones, respectively. A total of 13,057 putative orthologs were retained from each transcriptome in abalones. Overall Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyzed in each database showed a similar composition between sexes. In addition, a total of 519 and 391 isoforms were genome-widely identified with at least two isoforms from female and male transcriptome databases. We found that the number of isoforms and their alternatively spliced patterns are variable and sex-dependent. This information represents the first significant contribution to sex-preferential genomic resources of the Pacific abalone. The availability of whole female and male transcriptome database and their isoform information will be useful to improve our understanding of molecular responses and also for the analysis of population dynamics in the Pacific abalone.

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“…LTF has similar functions to ovotransferrin and is found in most milk and tear secretions in mammals 20 . Bacterial challenge causes a significant increase in tf / Tf expression in fish 21–23 , amphioxus 24 , crustaceans 25,26 , and mollusks 28 . Levels of iron in the serum and hemolymph are decreased significantly with heightened TF expression after bacterial challenge in Wuchang bream 22 and tobacco hawk moth 27 .…”

Section: Discussionmentioning

confidence: 99%

“…The precise function of MELTF remains unknown. Bacterial challenge causes a significant increase in TF / Tf / tf expression in fish 21–23 , amphioxus 24 , crustaceans 25,26 , insects 27 , and mollusks 28 . Thus, TF may play an important antimicrobial role through iron sequestration in teleost and invertebrates.…”

Section: Introductionmentioning

confidence: 99%

The Characteristics and Expression Profile of Transferrin in the Accessory Nidamental Gland of the Bigfin Reef Squid during Bacteria Transmission

Chen

Kuo

et al. 2019

Sci Rep

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The accessory nidamental gland (ANG) is a female reproductive organ found in most squid and cuttlefish that contains a consortium of bacteria. These symbiotic bacteria are transmitted from the marine environment and selected by the host through an unknown mechanism. In animals, a common antimicrobial mechanism of innate immunity is iron sequestration, which is based on the development of transferrin (TF)-like proteins. To understand this mechanism of host-microbe interaction, we attempted to characterize the role of transferrin in bigfin reef squid (Sepioteuthis lessoniana) during bacterial transmission. qPCR analysis showed that Tf was exclusively expressed in the outer layer of ANG,and this was confirmed by in situ hybridization, which showed that Tf was localized in the outer epithelial cell layer of the ANG. Western blot analysis indicated that TF is a soluble glycoprotein. Immunohistochemical staining also showed that TF is localized in the outer epithelial cell layer of the ANG and that it is mainly expressed in the outer layer during ANG growth. These results suggest that robust Tf mRNA and TF protein expression in the outer layer of the ANG plays an important role in microbe selection by the host during bacterial transmission.

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Molecular insights into a molluscan transferrin homolog identified from disk abalone (Haliotis discus discus) evidencing its detectable role in host antibacterial defense

Cited by 9 publications

References 59 publications

In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

Alternative Splicing Profile and Sex-Preferential Gene Expression in the Female and Male Pacific Abalone Haliotis discus hannai

The Characteristics and Expression Profile of Transferrin in the Accessory Nidamental Gland of the Bigfin Reef Squid during Bacteria Transmission

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